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1.发明申请Method of Preparing an Immunologically-Active Adjuvant-Bound Dried Vaccine Composition 有权制备免疫活性佐剂干燥疫苗组合物的方法公开(公告)号：US20100158951A1
公开(公告)日：2010-06-24
申请号：US12532225
申请日：2008-03-18
CPC分类号： A61K47/48015 , A61K9/19 , A61K39/08 , A61K2039/55505 , A61K2039/55511 , A61K2039/60 , A61K2039/622 , Y02A50/469
摘要： The disclosure provides a method of preparing an immunologically-active adjuvant-bound freeze dried vaccine composition. A specific embodiment provides a stable vaccine composition comprising an aluminum-salt adjuvant, a recombinant Clostridium botulinum neurotoxin protein and a glass-forming agent. These vaccine compositions are useful in the treatment of humans and other animals at risk of infection from Clostridium botulinum neurotoxin.
摘要翻译： 本公开提供了制备免疫学活性佐剂结合的冷冻干燥疫苗组合物的方法。 具体实施方案提供了包含铝盐佐剂，重组肉毒杆菌神经毒素蛋白质和玻璃形成剂的稳定的疫苗组合物。 这些疫苗组合物可用于治疗肉毒杆菌神经毒素感染风险的人和其他动物。
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2.发明授权Method of preparing an immunologically-active adjuvant-bound dried vaccine composition 有权制备免疫活性佐剂结合的干燥疫苗组合物的方法公开(公告)号：US08444991B2
公开(公告)日：2013-05-21
申请号：US12532225
申请日：2008-03-18
CPC分类号： A61K47/48015 , A61K9/19 , A61K39/08 , A61K2039/55505 , A61K2039/55511 , A61K2039/60 , A61K2039/622 , Y02A50/469
摘要： The disclosure provides a method of preparing an immunologically-active adjuvant-bound freeze dried vaccine composition. A specific embodiment provides a stable vaccine composition comprising an aluminum-salt adjuvant, a recombinant Clostridium botulinum neurotoxin protein and a glass-forming agent. These vaccine compositions are useful in the treatment of humans and other animals at risk of infection from Clostridium botulinum neurotoxin.
摘要翻译： 本公开提供了制备免疫学活性佐剂结合的冷冻干燥疫苗组合物的方法。 具体实施方案提供了包含铝盐佐剂，重组肉毒杆菌神经毒素蛋白质和玻璃形成剂的稳定的疫苗组合物。 这些疫苗组合物可用于治疗肉毒杆菌神经毒素感染风险的人和其他动物。
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公开(公告)号：US20110243996A1
公开(公告)日：2011-10-06
申请号：US13065965
申请日：2011-04-01
CPC分类号： A61K9/0043 , A61K9/1617 , A61K9/1623 , A61K9/1658 , A61K9/1694
摘要： This invention provides methods and compositions to preserve bioactive materials, such as peptides, nucleic acids, viruses, bacteria, cells, or liposomes, in freeze dried particles suitable for intranasal administration. Methods provide spray freeze drying of formulations to form stable freeze dried particles for intranasal administration.
摘要翻译： 本发明提供在适于鼻内给药的冷冻干燥颗粒中保存生物活性物质如肽，核酸，病毒，细菌，细胞或脂质体的方法和组合物。 方法提供制剂的喷雾冷冻干燥以形成用于鼻内给药的稳定的冷冻干燥颗粒。
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公开(公告)号：US20100330162A1
公开(公告)日：2010-12-30
申请号：US12807343
申请日：2010-09-01
IPC分类号： A61K9/127 , A61K39/145 , A61K9/14 , A61P31/12 , A61P31/04
CPC分类号： A61K9/0075 , A01N1/02 , A01N1/0252 , A61K9/1694 , A61K9/19
摘要： This invention provides methods and compositions to preserve bioactive materials, such as viruses, bacteria, cells, or liposomes, in freeze dried particles suitable for pulmonary administration. Methods provide spray freeze drying of formulations to form stable freeze dried particles.
摘要翻译： 本发明提供了在适于肺部给药的冷冻干燥颗粒中保存生物活性物质如病毒，细菌，细胞或脂质体的方法和组合物。 方法提供喷雾冷冻干燥制剂以形成稳定的冻干颗粒。
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公开(公告)号：US06489450B2
公开(公告)日：2002-12-03
申请号：US09350327
申请日：1999-07-09
IPC分类号： A23J100
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译： 本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。 重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。 通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。 该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。
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公开(公告)号：US07923029B2
公开(公告)日：2011-04-12
申请号：US10412644
申请日：2003-04-10
CPC分类号： A61K9/0075 , A01N1/02 , A01N1/0252 , A61K9/1694 , A61K9/19
摘要： This invention provides methods and compositions to preserve bioactive materials, such as viruses, bacteria, cells, or liposomes, in freeze dried particles suitable for pulmonary administration. Methods provide spray freeze drying of formulations to form stable freeze dried particles.
摘要翻译： 本发明提供了在适于肺部施用的冷冻干燥颗粒中保存生物活性物质如病毒，细菌，细胞或脂质体的方法和组合物。 方法提供喷雾冷冻干燥制剂以形成稳定的冻干颗粒。
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公开(公告)号：US20080161242A1
公开(公告)日：2008-07-03
申请号：US11901613
申请日：2007-09-17
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John , Lyndal K. Hesterberg , Matthew B. Seefeldt
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John , Lyndal K. Hesterberg , Matthew B. Seefeldt
IPC分类号： A61K38/00
CPC分类号： C07K1/1136 , A61K38/1774 , A61K38/215 , A61K38/27
摘要： Protein compositions with reduced immunogenicity are disclosed, as well as methods for producing such compositions.
摘要翻译： 公开了具有降低的免疫原性的蛋白质组合物，以及生产这种组合物的方法。
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公开(公告)号：US07064192B2
公开(公告)日：2006-06-20
申请号：US10292692
申请日：2002-11-12
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
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9.发明授权High-pressure inclusion body solubilization and protease clipping of recombinant fusion proteins 有权重组融合蛋白的高压包涵体溶解和蛋白酶剪切公开(公告)号：US07829681B2
公开(公告)日：2010-11-09
申请号：US12098877
申请日：2008-04-07
摘要： Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
摘要翻译： 本文描述了在大于大气压的压力下溶解和蛋白水解切割融合蛋白聚集体（包括自催化融合蛋白）以产生可溶性靶多肽的方法。
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公开(公告)号：US07767795B2
公开(公告)日：2010-08-03
申请号：US11341013
申请日：2006-01-27
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译： 本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。 重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。 通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。 该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。
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