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1.发明申请公开(公告)号：US20140138535A1
公开(公告)日：2014-05-22
申请号：US14084977
申请日：2013-11-20
申请人： Thermo Finnigan LLC
CPC分类号： H01J49/0036 , G16C20/20 , G16C20/90 , H01J49/26
摘要： A method of acquiring and interpreting data using (i) a mass spectrometer system operated according to a set of operating conditions and (ii) a mass spectral library having a plurality of library entries derived from data previously obtained using said mass spectrometer system operated according to said set of operating conditions comprising: (a) generating, using the mass spectrometer system, a multiplexed mass spectrum comprising a superposition of a plurality of product-ion mass spectra comprising a plurality of product-ion types, each product-ion mass spectrum corresponding to fragmentation of a respective precursor-ion type formed by ionization of the plurality of chemical compounds, each precursor-ion type having a respective precursor-ion mass-to-charge (m/z) ratio and each product ion type having a respective product-ion m/z ratio; and (b) decomposing the multiplexed product-ion mass spectrum, using the mass-spectral library, so as to calculate relative abundances of previously-observed product-ion mass spectra within the multiplexed product-ion mass spectrum.
摘要翻译： 一种使用（i）根据一组操作条件操作的质谱仪系统获取和解释数据的方法，以及（ii）具有多个库条目的质谱库，所述质谱库具有从先前根据根据 所述一组操作条件包括：（a）使用质谱仪系统生成包含多个产物离子质谱的叠加的多重质谱，所述多个产物离子质谱包括多个产物离子类型，每个产物离子质谱对应 通过多种化合物的离子化形成的各自的前体离子型的分裂，每种前体离子型具有各自的前体离子质荷比（m / z）比，并且每种产物离子型具有各自的产物 -ion m / z比; 和（b）使用质谱库分解多重产物离子质谱，以便计算多重产物离子质谱中先前观察到的产物离子质谱的相对丰度。
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公开(公告)号：US20160035549A1
公开(公告)日：2016-02-04
申请号：US14776910
申请日：2014-03-07
申请人： THERMO FINNIGAN LLC
发明人： Michael W. SENKO , Justin BLETHROW , Shannon Eliuk DIXON , Tonya SECOND , Vladimir ZABROUSKOV , Eric HEMENWAY
IPC分类号： H01J49/00
CPC分类号： H01J49/0031 , H01J49/005 , H01J49/009 , H01J49/061
摘要： A hybrid mass spectrometer design and architecture, and methods of operating mass spectrometers are disclosed. According to one operating method, an analysis time is determined for each one of a plurality of ion species to be analyzed in an ordered sequence, and an injection time is calculated for at least some of the ion species based on an analysis time of a preceding ion species in the ordered list. The method enables more efficient utilization of analyzer time.
摘要翻译： 公开了混合质谱仪设计和结构以及操作质谱仪的方法。 根据一种操作方法，以有序序列确定要分析的多个离子种类中的每一种的分析时间，并且基于前面的分析时间为至少一些离子种类计算注入时间 有序列表中的离子物种。 该方法可以更有效地利用分析仪的时间。
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3.发明申请公开(公告)号：US20150126406A1
公开(公告)日：2015-05-07
申请号：US14574854
申请日：2014-12-18
申请人： Thermo Finnigan LLC
CPC分类号： G01N33/58 , G01N27/62 , G01N33/6848 , G01N2458/15 , G01N2560/00 , Y10T436/24
摘要： Disclosed herein are isobaric labeling reagent sets useful for multiplexed quantitation of peptides. The isobaric labeling reagent sets include a collection of at least two isobaric labeling reagents having first and second reporter groups with the same nominal mass but different isotopic substitutions and consequently different exact masses. Mass spectrometric analysis of the labeled samples is performed using a mass analyzer, such as an Orbitrap mass analyzer, capable of adequately resolving the ions of the first and second reporter groups. Reagent sets of the foregoing description may provide a degree of multiplexing in reporter ion quantitation experiments that is expanded relative to conventional labeling reagent sets, thereby reducing the number of chromatographic runs required for analysis and improving sample throughput.
摘要翻译： 本文公开了用于多肽定量肽的同量异序标记试剂盒。 等压标记试剂组包括至少两个具有相同标称质量但具有不同同位素取代并因此具有不同精确质量的具有第一和第二报告基团的等压标记试剂的集合。 标记样品的质谱分析使用能够充分分辨第一和第二报道基团的离子的质谱仪（例如Orbitrap质谱仪）来进行。 上述描述的试剂组可以提供相对于常规标记试剂组扩增的报道子离子定量实验中的多重性，从而减少分析所需的色谱运行次数并提高样品产量。
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公开(公告)号：US20180068838A1
公开(公告)日：2018-03-08
申请号：US15811530
申请日：2017-11-13
申请人： Thermo Finnigan LLC
发明人： Michael W. SENKO , Justin BLETHROW , Shannon Eliuk DIXON , Tonya SECOND , Vladimir ZABROUSKOV , Eric HEMENWAY
CPC分类号： H01J49/0031 , H01J49/005 , H01J49/009 , H01J49/061
摘要： A hybrid mass spectrometer design and architecture, and methods of operating mass spectrometers are disclosed. According to one operating method, an analysis time is determined for each one of a plurality of ion species to be analyzed in an ordered sequence, and an injection time is calculated for at least some of the ion species based on an analysis time of a preceding ion species in the ordered list. The method enables more efficient utilization of analyzer time.
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5.发明申请Methods for Generating Local Mass Spectral Libraries for Interpreting Multiplexed Mass Spectra 审中-公开用于生成用于解释复用质谱的局部质谱库的方法公开(公告)号：US20140138537A1
公开(公告)日：2014-05-22
申请号：US14085356
申请日：2013-11-20
申请人： THERMO FINNIGAN LLC
IPC分类号： H01J49/00
CPC分类号： H01J49/0036 , G16C20/20 , G16C20/90 , H01J49/26
摘要： A method of acquiring and compiling data obtained on a mass spectrometer system, comprises: (a) generating a multiplexed mass spectrum comprising a superposed plurality of product-ion mass spectra comprising a plurality of product-ion types, each product-ion mass spectrum corresponding to fragmentation of a respective precursor-ion type, each precursor-ion type and each product ion type having a respective mass-to-charge (m/z) ratio; (b) decomposing the multiplexed product-ion mass spectrum so as to recognize relative abundances of previously-observed product-ion mass spectra within the multiplexed product-ion mass spectrum, the decomposing employing a mass-spectral library having a plurality of entries corresponding to respective product ion mass spectra previously-observed on said mass spectrometer system; (c) recognizing an additional contribution to the multiplexed mass spectrum that is neither attributable to random variation nor to any previously-observed product-ion spectrum; and (d) storing at least one new entry in the mass-spectral library relating to the recognized additional contribution.
摘要翻译： 一种获取和编辑在质谱仪系统上获得的数据的方法，包括：（a）产生多重质谱，其包括叠加的多个产物离子质谱，其包含多个产物离子类型，每个产物离子质谱对应 每个前体离子型和每种产物离子型具有相应的质荷比（m / z）比; （b）分解多重产物离子质谱，以便识别多重产物离子质谱中先前观察到的产物离子质谱的相对丰度，使用具有对应于多个产物离子质谱的多个条目的质谱库 在所述质谱系统上先前观察到各自的产物离子质谱; （c）识别对归因于随机变化的多重质谱的任何额外贡献，以及任何先前观察到的产物离子谱; 和（d）在质谱库中存储与所识别的附加贡献有关的至少一个新条目。
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6.发明申请公开(公告)号：US20160169902A1
公开(公告)日：2016-06-16
申请号：US14968874
申请日：2015-12-14
申请人： Thermo Finnigan LLC
CPC分类号： G01N33/58 , G01N27/62 , G01N33/6848 , G01N2458/15 , G01N2560/00 , Y10T436/24
摘要： Disclosed herein are isobaric labeling reagent sets useful for multiplexed quantitation of peptides. The isobaric labeling reagent sets include a collection of at least two isobaric labeling reagents having first and second reporter groups with the same nominal mass but different isotopic substitutions and consequently different exact masses. Mass spectrometric analysis of the labeled samples is performed using a mass analyzer, such as an Orbitrap mass analyzer, capable of adequately resolving the ions of the first and second reporter groups. Reagent sets of the foregoing description may provide a degree of multiplexing in reporter ion quantitation experiments that is expanded relative to conventional labeling reagent sets, thereby reducing the number of chromatographic runs required for analysis and improving sample throughput.
摘要翻译： 本文公开了用于多肽定量肽的同量异序标记试剂盒。 等压标记试剂组包括至少两个具有相同标称质量但具有不同同位素取代并因此具有不同精确质量的具有第一和第二报告基团的等压标记试剂的集合。 标记样品的质谱分析使用能够充分分辨第一和第二报道基团的离子的质谱仪（例如Orbitrap质谱仪）来进行。 上述描述的试剂组可以提供相对于常规标记试剂组扩增的报道子离子定量实验中的多重性，从而减少分析所需的色谱运行次数并提高样品产量。
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7.发明申请公开(公告)号：US20160042937A1
公开(公告)日：2016-02-11
申请号：US14885755
申请日：2015-10-16
申请人： Thermo Finnigan LLC
IPC分类号： H01J49/42
CPC分类号： H01J49/4265 , H01J49/0009 , H01J49/0027 , H01J49/0031 , H01J49/0036 , H01J49/004 , H01J49/26
摘要： A method for mass analyzing ions comprising a restricted range mass-to-charge (m/z) ratios comprising (i) performing a survey mass analysis, using a first mass analyzer employing indirect detection of ions by image current detection, to measure a flux of ions having m/z ratios within said range and (ii) performing a dependent mass analysis, using a second mass analyzer, of an optimal quantity of ions having m/z ratios within said range, said optimal quantity collected for a time period determined by the measured ion flux, the method characterized in that: the time period is determined using a corrected ion flux that includes a correction that comprises an estimate of the quantity of ions that are undetected by the first mass analyzer.
摘要翻译： 一种用于质量分析离子的方法，包括受限范围的质荷比（m / z）比，其包括：（i）使用通过图像电流检测间接检测离子的第一质量分析器进行测量质量分析，以测量通量 的离子，其m / z比在所述范围内，并且（ii）使用第二质量分析器进行在所述范围内具有m / z比的最佳离子量的依赖质量分析，所述最佳量在所确定的时间段内收集 通过测量的离子通量，该方法的特征在于：使用包括校正的校正离子通量来确定时间段，该校正包括由第一质量分析器未检测到的离子量的估计。
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8.发明申请公开(公告)号：US20140361158A1
公开(公告)日：2014-12-11
申请号：US14297092
申请日：2014-06-05
申请人： Thermo Finnigan LLC
CPC分类号： H01J49/4265 , H01J49/0009 , H01J49/0027 , H01J49/0031 , H01J49/0036 , H01J49/004 , H01J49/26
摘要： A method for mass analyzing ions comprising a restricted range mass-to-charge (m/z) ratios comprising (i) performing a survey mass analysis, using a first mass analyzer employing indirect detection of ions by image current detection, to measure a flux of ions having m/z ratios within said range and (ii) performing a dependent mass analysis, using a second mass analyzer, of an optimal quantity of ions having m/z ratios within said range, said optimal quantity collected for a time period determined by the measured ion flux, the method characterized in that: the time period is determined using a corrected ion flux that includes a correction that comprises an estimate of the quantity of ions that are undetected by the first mass analyzer.
摘要翻译： 一种用于质量分析离子的方法，包括受限范围的质荷比（m / z）比，其包括：（i）使用通过图像电流检测间接检测离子的第一质量分析器进行测量质量分析，以测量通量 的离子，其m / z比在所述范围内，并且（ii）使用第二质量分析器进行在所述范围内具有m / z比的最佳离子量的依赖质量分析，所述最佳量在所确定的时间段内收集 通过测量的离子通量，该方法的特征在于：使用包括校正的校正离子通量来确定时间段，该校正包括由第一质量分析器未检测到的离子量的估计。
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