-
1.发明授权Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more DNA's using primers having matched melting temperatures 失效用于使用具有匹配熔融温度的引物扩增和检测两种或多种DNA的诊断组合物，元件，方法和测试试剂盒
公开(公告)号：US5702901A
公开(公告)日：1997-12-30
申请号：US495739
申请日：1995-06-08
申请人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W.H. Sutherland , JoAnne H. Kerschner
发明人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W.H. Sutherland , JoAnne H. Kerschner
CPC分类号： C12Q1/708 , C12Q1/689 , C12Q1/703 , C12Q1/705 , C12Q2600/16 , Y10S977/728 , Y10S977/795 , Y10S977/836 , Y10S977/924 , Y10S977/958
摘要： An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.
摘要翻译： 含有两个或多个靶核酸相对链的引物的水性组合物可用于聚合酶链式反应，以提供对这些核酸的同时快速和有效的扩增和检测。 每个目标DNA的引物长度不同于5个核苷酸，Tm在约65-74℃范围内，而Tm在约5℃以内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多种捕获探针进行“复用”。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。
-
公开(公告)号：US5733751A
公开(公告)日：1998-03-31
申请号：US495741
申请日：1995-06-08
申请人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
发明人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
CPC分类号： C12Q1/708 , C12Q1/689 , C12Q1/703 , C12Q1/705 , C12Q2600/16 , Y10S977/728 , Y10S977/795 , Y10S977/836 , Y10S977/924 , Y10S977/958
摘要： An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.
-
公开(公告)号：US5654416A
公开(公告)日：1997-08-05
申请号：US495743
申请日：1995-06-08
申请人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
发明人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
CPC分类号： C12Q1/708 , C12Q1/689 , C12Q1/703 , C12Q1/705 , C12Q2600/16 , Y10S977/728 , Y10S977/795 , Y10S977/836 , Y10S977/924 , Y10S977/958
摘要： An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.
摘要翻译： 含有两个或多个靶核酸相对链的引物的水性组合物可用于聚合酶链式反应，以提供对这些核酸的同时快速和有效的扩增和检测。 每个目标DNA的引物长度不同于5个核苷酸，Tm在约65-74℃范围内，而Tm在约5℃以内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多种捕获探针进行“复用”。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。
-
4.发明授权Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more DNA's using primers having matched melting temperatures 失效用于使用具有匹配熔融温度的引物扩增和检测两种或多种DNA的诊断组合物，元件，方法和测试试剂盒公开(公告)号：US06936415B1
公开(公告)日：2005-08-30
申请号：US09675828
申请日：2000-09-29
申请人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
发明人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
CPC分类号： C12Q1/708 , C12Q1/689 , C12Q1/703 , C12Q1/705 , C12Q2600/16 , Y10S977/728 , Y10S977/795 , Y10S977/836 , Y10S977/924 , Y10S977/958
摘要： An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from about 65 to about 74° C., while the Tm's are within about 5° C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in “multiplexing”, using multiple capture probes. All of the capture probes have Tm's which are greater than 50° C. and are within 15° C. of each other.
摘要翻译： 含有两个或多个靶核酸相对链的引物的水性组合物可用于聚合酶链式反应，以提供对这些核酸的同时快速和有效的扩增和检测。 每个靶DNA的引物的长度不同于5个核苷酸，T < SUB>彼此在约5℃以内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多种捕获探针进行“复用”。 所有的捕获探针具有大于50℃并且彼此在15℃以内的T m。
-
5.发明授权Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more target DNA's using primers having matched melting temperatures 失效用于使用具有匹配熔融温度的引物扩增和检测两种或多种靶DNA的诊断组合物，元件，方法和测试试剂盒公开(公告)号：US06174668B1
公开(公告)日：2001-01-16
申请号：US08062023
申请日：1993-05-14
申请人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
发明人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
IPC分类号： C12Q168
CPC分类号： C12Q1/708 , C12Q1/689 , C12Q1/703 , C12Q1/705 , C12Q2600/16 , Y10S977/728 , Y10S977/795 , Y10S977/836 , Y10S977/924 , Y10S977/958
摘要： An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from about 65 to about 74° C., while the Tm's are within about 5° C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in “multiplexing”, using multiple capture probes. All of the capture probes have Tm's which are greater than 50° C. and are within 15° C. of each other.
摘要翻译： 含有两个或多个靶核酸相对链的引物的水性组合物可用于聚合酶链式反应，以提供对这些核酸的同时快速和有效的扩增和检测。 每个靶DNA的引物长度不同于5个核苷酸，并且Tm在约65至约74℃的范围内，而Tm在彼此的约5℃内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多种捕获探针进行“复用”。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。
-
6.发明授权Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more DNA's using primers having matched melting temperatures 失效用于使用具有匹配熔融温度的引物扩增和检测两种或多种DNA的诊断组合物，元件，方法和测试试剂盒
公开(公告)号：US5811295A
公开(公告)日：1998-09-22
申请号：US495742
申请日：1995-06-08
申请人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
发明人： Thomas J. Cummins , Susan Melissa Atwood , Lynn Bergmeyer , John Bruce Findlay , John W. H. Sutherland , JoAnne H. Kerschner
CPC分类号： C12Q1/708 , C12Q1/689 , C12Q1/703 , C12Q1/705 , C12Q2600/16 , Y10S977/728 , Y10S977/795 , Y10S977/836 , Y10S977/924 , Y10S977/958
摘要： An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.
摘要翻译： 含有两个或多个靶核酸相对链的引物的水性组合物可用于聚合酶链式反应，以提供对这些核酸的同时快速和有效的扩增和检测。 每个目标DNA的引物长度不同于5个核苷酸，Tm在约65-74℃范围内，而Tm在约5℃以内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多种捕获探针进行“复用”。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。
-
7.发明授权Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures 失效使用具有匹配熔融温度的引物扩增和检测人CMV DNA的诊断组合物，元件，方法和测试试剂盒公开(公告)号：US06709813B1
公开(公告)日：2004-03-23
申请号：US08062021
申请日：1993-05-14
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/686
摘要： An aqueous composition containing primers for opposing strands of human cytomegaloviral DNA and a second target DNA can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from about 65 to about 74° C., while the Tm's are within about 5° C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of hCMV DNA and other target DNA's using multiple capture probes, in “multiplexing”. All of the capture probes have Tm's which are greater than 50° C. and are within 15° C. of each other.
摘要翻译： 含有针对人类巨细胞病毒DNA和第二靶DNA的引物的含水组合物可以用于聚合酶链式反应中以提供对这些核酸的同时快速和有效的扩增和检测。 每个靶DNA的引物长度不同于5个核苷酸，并且Tm在约65至约74℃的范围内，而Tm在彼此的约5℃内。 这样的组合物可用于诊断测试试剂盒和用于在“多路复用”中使用多个捕获探针扩增和检测hCMV DNA和其他靶DNA的方法。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。
-
8.发明授权Method, test element and test kit for semi-quantitative detection of target nucleic acid 失效用于半定量检测靶核酸的方法，测试元件和测试试剂盒
公开(公告)号：US5591580A
公开(公告)日：1997-01-07
申请号：US221818
申请日：1994-03-31
申请人： Lynn Bergmeyer , Thomas J. Cummins
发明人： Lynn Bergmeyer , Thomas J. Cummins
CPC分类号： C12Q1/6834 , C12Q1/6813 , C12Q1/686
摘要： A target nucleic acid can be detected in a semi-quantitative fashion by passing it over detection deposits in a test element. The detection deposits include particles affixed to the test element, some of which particles have capture probe attached thereto, and other particles having no capture probe. The deposits have varying amounts of capture probe so that the signal obtained when the target nucleic acid is captured thereon can be semi-quantitatively correlated to the amount of target nucleic acid in the specimen. This method of detection can be used in nucleic acid hybridization assays or following amplification methods, including polymerase chain reaction.
摘要翻译： 靶核酸可以通过将其超过检测元件中的检测沉积物以半定量方式检测。 检测沉积物包括固定在测试元件上的颗粒，其中一些颗粒具有附着于其上的捕获探针和其它没有捕获探针的颗粒。 沉积物具有不同量的捕获探针，使得当靶核酸被捕获时获得的信号可以与样品中靶核酸的量半定量相关。 该检测方法可用于核酸杂交测定或扩增方法，包括聚合酶链反应。
-
公开(公告)号：US5422271A
公开(公告)日：1995-06-06
申请号：US979569
申请日：1992-11-20
CPC分类号： B01L3/502
摘要： A device and method are disclosed for amplifying and detecting nucleic acid material. The device and method use a label and signalling material responsive to the label to produce a detectable signal. A surprising result of the method and device is that at least one of the wash steps heretofore required has been eliminated without substantially adversely affecting the results.
摘要翻译： 公开了用于扩增和检测核酸材料的装置和方法。 该装置和方法使用响应标签的标签和信令材料产生可检测信号。 该方法和装置的令人惊奇的结果是至少需要一个洗涤步骤已被消除，而不会对结果产生实质性的不利影响。
-
10.发明授权Diagnostic and amplification methods using primers having thymine at 3' end to overcome primer-target mismatch at the 3' end 失效在3'末端使用3'末端终止重载目标错误的引导符的诊断和放大方法
公开(公告)号：US5196305A
公开(公告)日：1993-03-23
申请号：US406221
申请日：1989-09-12
申请人： John B. Findlay , Lynn Bergmeyer
发明人： John B. Findlay , Lynn Bergmeyer
CPC分类号： C12Q1/6858 , C12Q1/703 , Y10S435/805 , Y10S435/948 , Y10S436/811
摘要： Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are accomplished even where there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3' end of the primer. Such a mismatch is overcome using a primer having a nucleotide with a thymine base at the position of the mismatch. The use of such primers is most likely to prime the target and form primer extension products. This method is particularly useful for detection of a nucleic acid sequence which is not fully known, or where there is considerable heterogeneity in DNA target from patient samples.
-
-
-
-
-
-
-
-
-
搜索结果
11 条记录 (耗时 0.036 秒)
