公开(公告)号：US5766884A

公开(公告)日：1998-06-16

申请号：US466443

申请日：1995-06-06

申请人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

发明人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  C12N15/00 ,  C12N15/79

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/0306 ,  A01K2267/0381 ,  A61K38/00 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/85 ,  Y10S435/948

摘要： The present invention relates to the synthesis of functional human hemoglobin and other proteins in erythroid tissues of transgenic non-human animals and erythroid cell lines. It is based on the discovery that two of the five hypersensitivity sites of the .beta.-globin locus are sufficient to result in high level expression of human .alpha.- or .beta.-globin transgenes. The present invention also provides for novel recombinant nucleic acid vectors which may be used to produce .alpha.-globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the .beta.-globin locus together with a gene of interest. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human .alpha.-globin gene is used to create transgenic animals which produce human .alpha.-globin protein in erythroid tissues, including red blood cells. In a preferred specific embodiment of the invention, transgenic animals are created which comprise human .alpha.-globin and .beta.-globin genes, each under the transcriptional influence of two .beta.-globin locus DNase hypersensitivity sites; these transgenic animals express human hemoglobin in their erythroid tissues, and can be used to produce human hemoglobin in quantity.

摘要翻译： 本发明涉及转基因非人动物和红系细胞系的红细胞组织中功能性人血红蛋白和其他蛋白质的合成。 基于以下发现：β-珠蛋白基因座的五个超敏性位点中的两个足以导致人α或β-珠蛋白转基因的高水平表达。 本发明还提供新的重组核酸载体，其可用于在转基因动物或红细胞谱系的细胞培养物的红细胞中产生量的α-珠蛋白以及其它目标蛋白。 本发明的载体包含与β-球蛋白基因座相关联的主要DNA酶I超敏反应位点与感兴趣的基因中的至少一种。 在本发明的一个具体实施方案中，使用包含两个DNA酶I超敏反应位点与人α-珠蛋白基因一起的载体来产生在包括红细胞在内的红细胞组织中产生人α-珠蛋白的转基因动物。 在本发明的优选具体实施方案中，产生包含人α-珠蛋白和β-珠蛋白基因的转基因动物，每个基因在两个β-球蛋白基因座DNA酶超敏位点的转录影响下; 这些转基因动物在其红细胞组织中表达人血红蛋白，并且可用于量产生人血红蛋白。

公开(公告)号：US06613957B1

公开(公告)日：2003-09-02

申请号：US09388401

申请日：1999-09-01

申请人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

发明人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

IPC分类号： A01K67027

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/0306 ,  A01K2267/0381 ,  A61K38/00 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/85 ,  Y10S435/948

摘要： The present invention relates to the synthesis of functional human hemoglobin and other proteins in erythroid tissues of transgenic non-human animals and erythroid cell lines. It is based on the discovery that two of the five hypersensitivity sites of the &bgr;-globin locus are sufficient to result in high level expression of human &agr;- or &bgr;-globin transgenes.

摘要翻译： 本发明涉及转基因非人动物和红系细胞系的红细胞组织中功能性人血红蛋白和其他蛋白质的合成。 基于以下发现：β珠蛋白基因座的五个超敏反应位点中的两个足以导致人α或β-珠蛋白转基因的高水平表达。

公开(公告)号：US6022736A

公开(公告)日：2000-02-08

申请号：US471483

申请日：1995-06-06

申请人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

发明人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  C12N15/63 ,  C12N5/00 ,  C12N15/00 ,  C12N15/09

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/0306 ,  A01K2267/0381 ,  A61K38/00 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/85 ,  Y10S435/948

摘要： The present invention provides novel recombinant nucleic acid vectors which may be used to produce .alpha.-globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The present invention also provides for the transgenic animals which contain these recombinant nucleic acid vectors. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the .beta.-globin locus together with a gene of interest. According to various embodiments of the invention, the vectors may be used to create transgenic animals or to transfect cells in culture. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human .alpha.-globin gene is used to create transgenic animals which produce human .alpha.-globin protein in erythroid tissues, including red blood cells. In a preferred specific embodiment of the invention, transgenic animals are created which comprise human .alpha.-globin and .beta.-globin genes, each under the transcriptional influence of two .beta.-globin locus DNase hypersensitivity sites; these transgenic animals express human hemoglobin in their erythroid tissues, and can be used to produce human hemoglobin in quantity.

摘要翻译： 本发明提供新的重组核酸载体，其可用于在转基因动物或红细胞谱系的细胞培养物的红细胞中产生量的α-珠蛋白以及其它目标蛋白。 本发明还提供含有这些重组核酸载体的转基因动物。 本发明的载体包含与β-球蛋白基因座相关联的主要DNA酶I超敏反应位点与感兴趣的基因中的至少一种。 根据本发明的各种实施方案，载体可用于产生转基因动物或转染培养物中的细胞。 在本发明的一个具体实施方案中，使用包含两个DNA酶I超敏反应位点与人α-珠蛋白基因一起的载体来产生在包括红细胞在内的红细胞组织中产生人α-珠蛋白的转基因动物。 在本发明的优选具体实施方案中，产生包含人α-珠蛋白和β-珠蛋白基因的转基因动物，每个基因在两个β-球蛋白基因座DNA酶超敏位点的转录影响下; 这些转基因动物在其红细胞组织中表达人血红蛋白，并且可用于量产生人血红蛋白。

公开(公告)号：US06405103B1

公开(公告)日：2002-06-11

申请号：US09216245

申请日：1998-12-18

申请人： Thomas M. Ryan ,  James M. Ryan ,  Louis A. Santiago

发明人： Thomas M. Ryan ,  James M. Ryan ,  Louis A. Santiago

IPC分类号： G05B1300

CPC分类号： G05B15/02 ,  F24F11/54 ,  G05B19/042 ,  G05B2219/23027 ,  G05B2219/2642

摘要： Disclosed is a system for controlling appliances of at least one building, each appliance being associated with a local controller and having a control input and at least one control point. The system includes a central processor computer having an operator interface and an equipment interface, a network connected to the equipment interface and having a plurality of nodes for communicating with each of the appliances, a log file being stored with respective area, description, and address fields containing corresponding data of the control points. A control program in the computer feeds the equipment interface to drive the control inputs of the appliances in response to a database, the database being populated from a tagname dictionary. A conversion program produces the tagname dictionary as a transfer file, the program including instructions for accessing the area, description, and address fields of the log file, generating for each point a tagname as a concatenation of the area and description fields, and an item including contents of the address field, and storing the tagnames and associated items with the header in the transfer file, whereby the system drives the control inputs of the appliances in response to the control point data of the log file. The conversion program can include a mode selector for directing conditional, unconditional and test modes of a utility process populating the database. Also disclosed is a process for controlling multiple appliances of at least one building.

摘要翻译： 公开了一种用于控制至少一个建筑物的设备的系统，每个设备与本地控制器相关联并具有控制输入和至少一个控制点。 该系统包括具有操作员接口和设备接口的中央处理器计算机，连接到设备接口的网络，并且具有用于与每个设备通信的多个节点，存储有相应区域，描述和地址的日志文件 包含控制点的相应数据的字段。 计算机中的控制程序馈送设备接口以响应于数据库驱动设备的控制输入，数据库从标记名字典填充。 转换程序产生标记名字典作为传送文件，该程序包括用于访问日志文件的区域，描述和地址字段的指令，为每个点生成标记名作为区域和描述字段的级联，以及项目 包括地址字段的内容，以及将标题名和与标题相关联的项目存储在传送文件中，由此系统响应于日志文件的控制点数据来驱动设备的控制输入。 转换程序可以包括用于指示填充数据库的实用程序的条件，无条件和测试模式的模式选择器。 还公开了一种用于控制至少一个建筑物的多个装置的过程。

公开(公告)号：US5602306A

公开(公告)日：1997-02-11

申请号：US275313

申请日：1994-07-13

申请人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

发明人： Tim M. Townes ,  Thomas M. Ryan ,  Richard D. Palmiter ,  Ralph L. Brinster ,  Richard R. Behringer

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  C12N15/00

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A61K48/00 ,  C07K14/805 ,  C12N15/85 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/0306 ,  A01K2267/0381 ,  A61K38/00 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/85 ,  Y10S435/948

摘要： The present invention relates to the synthesis of functional human hemoglobin and other proteins in erythroid tissues of transgenic non-human animals and erythroid cell lines. It is based on the discovery that two of the five hypersensitivity sites of the .beta.-globin locus are sufficient to result in high level expression of human .alpha.- or .beta.-globin transgenes. The present invention also provides for novel recombinant nucleic acid vectors which may be used to produce .alpha.-globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the .beta.-globin locus together with a gene of interest. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human .alpha.-globin gene is used to create transgenic animals which produce human .alpha.-globin protein in erythroid tissues, including red blood cells. In a preferred specific embodiment of the invention, transgenic animals are created which comprise human .alpha.-globin and .beta.-globin genes, each under the transcriptional influence of two .beta.-globin locus DNase hypersensitivity sites; these transgenic animals express human hemoglobin in their erythroid tissues, and can be used to produce human hemoglobin in quantity.

摘要翻译： 本发明涉及转基因非人动物和红系细胞系的红细胞组织中功能性人血红蛋白和其他蛋白质的合成。 基于以下发现：β-珠蛋白基因座的五个超敏性位点中的两个足以导致人α或β-珠蛋白转基因的高水平表达。 本发明还提供新的重组核酸载体，其可用于在转基因动物或红细胞谱系的细胞培养物的红细胞中产生量的α-珠蛋白以及其它目标蛋白。 本发明的载体包含与β-球蛋白基因座相关联的主要DNA酶I超敏反应位点与感兴趣的基因中的至少一种。 在本发明的一个具体实施方案中，使用包含两个DNA酶I超敏反应位点与人α-珠蛋白基因一起的载体来产生在包括红细胞在内的红细胞组织中产生人α-珠蛋白的转基因动物。 在本发明的优选具体实施方案中，产生包含人α-珠蛋白和β-珠蛋白基因的转基因动物，每个基因在两个β-球蛋白基因座DNA酶超敏位点的转录影响下; 这些转基因动物在其红细胞组织中表达人血红蛋白，并且可用于量产生人血红蛋白。

公开(公告)号：US5439271A

公开(公告)日：1995-08-08

申请号：US148316

申请日：1993-11-08

申请人： Thomas M. Ryan

发明人： Thomas M. Ryan

IPC分类号： B60N2/68 ,  A47C7/18

CPC分类号： B60N2/68

摘要： A vehicle seat in which the cushion frame is formed by extruded frame members that are extruded as a long rail and then cut to the desired length for the seat assembly. The remaining components of the seat frame are universal and can be used with a seat of any width. The seat structure enables the seat manufacturer to stock a minimum number of components for seats of varying widths and can easily change from production of seats of one width to seats of a different width.

摘要翻译： 一种车辆座椅，其中缓冲框架由挤出的框架构件形成，所述框架构件被挤压成长轨道，然后切割成用于座椅组件的期望长度。 座椅框架的其余部件是通用的，可以与任何宽度的座椅一起使用。 座椅结构使得座椅制造商能够存储不同宽度的座椅的最小数量的部件，并且可以容易地从一个宽度的座椅的生产变化到不同宽度的座椅。

公开(公告)号：US4949088A

公开(公告)日：1990-08-14

申请号：US396372

申请日：1989-08-21

申请人： Thomas M. Ryan ,  Karen Ryan

发明人： Thomas M. Ryan ,  Karen Ryan

IPC分类号： G01S3/48 ,  G01S7/02

CPC分类号： G01S7/022 ,  G01S3/48

摘要： A system includes units for identifying the location and range of a radar signal source relative to a user's vehicle. The system also includes an alarm unit for signalling if the radar source is within a prescribed distance from the vehicle. A readout and display unit displays selected information from the various units of the system.

摘要翻译： 系统包括用于识别雷达信号源相对于用户车辆的位置和范围的单元。 该系统还包括一个报警单元，用于在雷达源距离车辆一定距离内进行信令。 读出和显示单元从系统的各个单元显示选定的信息。