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公开(公告)号：US08691238B2
公开(公告)日：2014-04-08
申请号：US13140395
申请日：2009-12-18
申请人： Thomas Muster , Markus Wolschek , Andrej Egorov , Elisabeth Roethl , Julia Romanova , Michael Bergmann
发明人： Thomas Muster , Markus Wolschek , Andrej Egorov , Elisabeth Roethl , Julia Romanova , Michael Bergmann
IPC分类号： A61K39/145 , A61K39/12 , C12N7/08
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/525 , C07K14/005 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151
摘要： The present invention provides a high growth reassortant influenza A virus having at least two gene segments of seasonal or pandemic strain origin, a PB1 gene segment of A/Texas/1/77 strain origin and a PA gene segment of A/Puerto Rico/8/34 (H1N1) origin coding for a PA protein comprising at least one amino acid modification at any one of positions 10, 275, 682, according to SEQ ID No. 1. Further provided are vaccine formulations comprising the reassortant influenza A virus of the invention.
摘要翻译： 本发明提供了具有至少两个季节性或大流行菌株起源的基因片段，A / Texas / 1/77株源的PB1基因片段和A / Puerto Rico / 8的PA基因片段的高生长重配甲型流感病毒 编码PA蛋白的/ 34（H1N1）来源，其包含根据SEQ ID No.1的位置10,275,682中任一位置的至少一个氨基酸修饰。进一步提供的疫苗制剂包含重配甲型流感病毒 发明。
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公开(公告)号：US20110262481A1
公开(公告)日：2011-10-27
申请号：US13140395
申请日：2009-12-18
申请人： Thomas Muster , Markus Wolschek , Andrej Egorov , Elisabeth Roethl , Julia Romanova , Michael Bergmann
发明人： Thomas Muster , Markus Wolschek , Andrej Egorov , Elisabeth Roethl , Julia Romanova , Michael Bergmann
IPC分类号： A61K39/145 , A61P37/04 , A61P31/16 , C12N7/01 , C12N9/12
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/525 , C07K14/005 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151
摘要： The present invention provides a high growth reassortant influenza A virus having at least two gene segments of seasonal or pandemic strain origin, a PB1 gene segment of A/Texas/1/77 strain origin and a PA gene segment of A/Puerto Rico/8/34 (H1N1) origin coding for a PA protein comprising at least one amino acid modification at any one of positions 10, 275, 682, according to SEQ ID No.1. Further provided are vaccine formulations comprising the reassortant influenza A virus of the invention.
摘要翻译： 本发明提供了具有至少两个季节性或大流行菌株起源的基因片段，A / Texas / 1/77株源的PB1基因片段和A / Puerto Rico / 8的PA基因片段的高生长重配甲型流感病毒 / 34（H1N1）来源编码PA蛋白，其包含根据SEQ ID No.1的位置10,275,682中的任一个的至少一个氨基酸修饰。 还提供了包含本发明的重配甲型流感病毒的疫苗制剂。
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3.发明申请公开(公告)号：US20100129399A1
公开(公告)日：2010-05-27
申请号：US12666459
申请日：2008-06-26
IPC分类号： A61K39/145 , C12N15/63 , C12N5/00 , C12P21/00 , A61K35/76 , C12N7/00 , A61P31/16 , A61P37/00
CPC分类号： C12N15/86 , A61K2039/525 , A61K2039/5256 , C07K14/005 , C12N7/00 , C12N2760/16122 , C12N2760/16143 , C12N2760/16161
摘要： The present invention provides a linear expression construct free of any conventional amplification and/or selection sequences comprising an RNA polymerase I (polI) promoter and a polI termination signal, inserted between a RNA polymerase II (polII) promoter and a polyadenylation signal useful for the expression of segments of viral RNA, preferably influenza viruses. The inventive construct is useful for efficient and fast production of viral particles, especially for producing vaccine formulations for the treatment of epidemic and/or pandemic diseases.
摘要翻译： 本发明提供了不含任何常规扩增和/或选择序列的线性表达构建体，所述扩增和/或选择序列包含RNA聚合酶I（polI）启动子和polI终止信号，其插入在RNA聚合酶II（polII）启动子和聚腺苷酸化信号之间， 表达病毒RNA片段，优选流感病毒。 本发明的构建体可用于有效和快速地生产病毒颗粒，特别是用于生产用于治疗流行性和/或流行性疾病的疫苗制剂。
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4.发明申请REPLICATION DEFICIENT INFLUENZA VIRUS FOR THE EXPRESSION OF HETEROLOGOUS SEQUENCES 审中-公开用于表达异常序列的缺失性流感病毒公开(公告)号：US20100136052A1
公开(公告)日：2010-06-03
申请号：US12666246
申请日：2008-06-26
CPC分类号： C12N7/00 , A61K39/00 , A61K2039/525 , A61K2039/5256 , C07K14/005 , C12N15/86 , C12N2760/16132 , C12N2760/16143 , C12N2760/16243
摘要： The present invention covers a novel replication deficient influenza virus comprising a modified NS1 segment coding for a NS1 protein lacking a functional RNA binding domain and functional effector domain and a heterologous sequence inserted between the splice donor site and the splice acceptor site of the NS gene segment. Further the use of the virus as vector for expression of various proteins like chemokines, cytokines or antigenic structures is covered, methods for producing virus particles using said virus vector as well as its use for production of vaccines. Also a fusion peptide comprising part of the N-terminus of an NS1 protein and a signal sequence fused to the C-terminus of said NS1 peptide is covered.
摘要翻译： 本发明涵盖了一种新型复制缺陷型流感病毒，其包含编码NS1蛋白的修饰的NS1片段，其缺乏功能性RNA结合结构域和功能性效应结构域，以及插入在NS基因片段的剪接供体位点和剪接受体位点之间的异源序列 。 此外，涵盖了病毒作为用于表达各种蛋白质（如趋化因子，细胞因子或抗原结构）的载体的使用，使用所述病毒载体产生病毒颗粒的方法以及其用于生产疫苗的方法。 还包括包含NS1蛋白的N-末端的一部分和与所述NS1肽的C末端融合的信号序列的融合肽。
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公开(公告)号：US08815252B2
公开(公告)日：2014-08-26
申请号：US13129806
申请日：2009-11-25
IPC分类号： A61K39/145 , C12N7/00 , C12N7/08
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5254 , C12N7/00 , C12N2760/16021 , C12N2760/16034 , C12N2760/16051 , C12N2760/16134
摘要： The present invention provides a method for producing pH-stable enveloped viruses wherein said viruses are used for infection of host cells under low pH conditions and for incubation with cell culture cells under conditions of low pH, as well as influenza viruses obtainable by this method which exhibit a high growth rate in cell culture, increased pH and temperature stability and which have human receptor specificity.
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公开(公告)号：US08883479B2
公开(公告)日：2014-11-11
申请号：US12626475
申请日：2009-11-25
IPC分类号： A61K39/145 , C12N7/00 , C12N7/01 , A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5254 , C12N7/00 , C12N2760/16021 , C12N2760/16034 , C12N2760/16051 , C12N2760/16134
摘要： The present invention provides a method for producing pH-stable enveloped viruses wherein said viruses are used for infection of host cells under low pH conditions and for incubation with cell culture cells under conditions of low pH, as well as influenza viruses obtainable by this method which exhibit a high growth rate in cell culture, increased pH and temperature stability and which have human receptor specificity.
摘要翻译： 本发明提供一种生产pH稳定包膜病毒的方法，其中所述病毒用于在低pH条件下感染宿主细胞，并在低pH条件下与细胞培养细胞一起孵育，以及可通过该方法获得的流感病毒 在细胞培养物中表现出高生长速率，增加的pH和温度稳定性并且具有人受体特异性。
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公开(公告)号：US20110223199A1
公开(公告)日：2011-09-15
申请号：US13129806
申请日：2009-11-25
IPC分类号： A61K39/145 , C12N7/00 , C12N7/04 , A61P37/04 , A61P31/16
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5254 , C12N7/00 , C12N2760/16021 , C12N2760/16034 , C12N2760/16051 , C12N2760/16134
摘要： The present invention provides a method for producing pH-stable enveloped viruses wherein said viruses are used for infection of host cells under low pH conditions and for incubation with cell culture cells under conditions of low pH, as well as influenza viruses obtainable by this method which exhibit a high growth rate in cell culture, increased pH and temperature stability and which have human receptor specificity.
摘要翻译： 本发明提供一种生产pH稳定包膜病毒的方法，其中所述病毒用于在低pH条件下感染宿主细胞，并在低pH条件下与细胞培养细胞一起孵育，以及可通过该方法获得的流感病毒 在细胞培养物中表现出高生长速率，增加的pH和温度稳定性并且具有人受体特异性。
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公开(公告)号：US08741301B2
公开(公告)日：2014-06-03
申请号：US13132609
申请日：2009-12-03
申请人： Thomas Muster , Andrej Egorov , Markus Wolschek
发明人： Thomas Muster , Andrej Egorov , Markus Wolschek
IPC分类号： A61K39/00 , A61K39/145 , A61K39/12
CPC分类号： A61K39/00 , A61K39/12 , A61K39/145 , A61K2039/5254 , C07K14/005 , C07K2319/50 , C12N7/00 , C12N2760/16122 , C12N2760/16134 , C12N2760/16152 , C12N2770/20022 , C12N2830/205
摘要： The present invention provides a method for generating negative-stranded segmented RNA viruses using linear expression constructs in the presence of helper virus.
摘要翻译： 本发明提供了在辅助病毒存在下使用线性表达构建体产生负链分段的RNA病毒的方法。
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公开(公告)号：US20130183740A1
公开(公告)日：2013-07-18
申请号：US13701164
申请日：2011-06-06
申请人： Thomas Muster , Andrej Egorov , Markus Wolschek
发明人： Thomas Muster , Andrej Egorov , Markus Wolschek
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C07K2319/50 , C12N7/02 , C12N15/86 , C12N2760/00011 , C12N2760/00052 , C12N2760/16111 , C12N2760/16134 , C12N2760/16152
摘要： The present invention provides a method for generating negative-stranded segmented RNA viruses using linear expression constructs in the presence of helper virus which comprises at least one amino acid modification within the N-terminal cyto plasmic region of the NA protein.
摘要翻译： 本发明提供了在辅助病毒存在下使用线性表达构建体产生负链分段RNA病毒的方法，所述辅助病毒包含在NA蛋白的N末端细胞质量区内的至少一个氨基酸修饰。
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公开(公告)号：US20110250587A1
公开(公告)日：2011-10-13
申请号：US13132609
申请日：2009-12-03
申请人： Thomas Muster , Andrej Egorov , Markus Wolschek
发明人： Thomas Muster , Andrej Egorov , Markus Wolschek
CPC分类号： A61K39/00 , A61K39/12 , A61K39/145 , A61K2039/5254 , C07K14/005 , C07K2319/50 , C12N7/00 , C12N2760/16122 , C12N2760/16134 , C12N2760/16152 , C12N2770/20022 , C12N2830/205
摘要： The present invention provides a method for generating negative-stranded segmented RNA viruses using linear expression constructs in the presence of helper virus.
摘要翻译： 本发明提供了在辅助病毒存在下使用线性表达构建体产生负链分段的RNA病毒的方法。
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