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    Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors
    4.
    发明授权
    Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors 有权

    公开(公告)号：US09890413B2

    公开(公告)日：2018-02-13

    申请号：US11338002

    申请日：2006-01-24

    申请人： Markus Sprenger-Haussels

    发明人： Markus Sprenger-Haussels

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q2527/137 ,  C12Q2527/119

    摘要： The invention related to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out this method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infection, in particular, bacterial or viral infection, or mutation, in particular, for tumor-specific DNA mutations.

    METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS
    5.
    发明申请
    METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS 有权
    用于分离短链核酸的方法

    公开(公告)号：US20110160446A1

    公开(公告)日：2011-06-30

    申请号：US12995341

    申请日：2009-05-12

    申请人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    发明人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    IPC分类号： C07H1/06

    CPC分类号： C07H1/08 ,  C12N15/1006

    摘要： The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.

    摘要翻译： 本发明涉及用于从含有起始物质的核酸分离和/或纯化核酸，特别是短链核酸的方法和试剂盒，其特征在于以下方法步骤：（a）将核酸结合至 核酸结合载体材料，其中原料在至少一种离液序列化合物和优选异丙醇的存在下与核酸键合载体材料接触，其中异丙醇以≥25％（v / v）的浓度存在 ）和＆lt; nlE; 35％（v / v），（b）从核酸粘合载体材料任选洗脱键合的核酸。 所述方法特别适用于从母体血液中纯化胎儿DNA。

    Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
    6.
    发明授权
    Nucleic acid isolation from stool samples and other inhibitor-rich biological materials 有权
    从粪便样品和其他抑制剂丰富的生物材料中分离核酸

    公开(公告)号：US07005266B2

    公开(公告)日：2006-02-28

    申请号：US10182950

    申请日：2001-01-18

    申请人： Markus Sprenger-Haussels

    发明人： Markus Sprenger-Haussels

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q2527/137 ,  C12Q2527/119

    摘要： The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out said method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infections, in particular, bacterial or viral infections, or mutations, in particular, for tumour-specific DNA mutations.

    摘要翻译： 本发明涉及从材料样品，特别是可以含有杂质和抑制剂或干扰物质的粪便样品中稳定，纯化或/和分离核酸的方法。 本发明还涉及用于执行所述方法的试剂盒。 本发明的基础尤其是用于从材料样品中纯化，稳定或/和分离核酸的方法，由此将缓冲液加入到含有核酸的样品中，pH值为2-7，a 盐浓度至少为100mM，或/和苯酚中和物质。 根据本发明，可以通过简单的方法从粪便样品获得可以扩增的纯核酸，其适用于感染的诊断性证据，特别是细菌或病毒感染或突变，特别是肿瘤特异性 DNA突变。

    Method for isolating nucleic acids
    7.
    发明授权
    Method for isolating nucleic acids 有权

    公开(公告)号：US09809612B2

    公开(公告)日：2017-11-07

    申请号：US12995348

    申请日：2009-05-12

    申请人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    发明人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    IPC分类号： C07H1/08 ,  C12N15/10

    CPC分类号： C07H1/08 ,  C12N15/1006

    摘要： The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterized by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧15% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.

    METHOD FOR ISOLATING NUCLEIC ACIDS
    9.
    发明申请
    METHOD FOR ISOLATING NUCLEIC ACIDS 有权
    分离核酸的方法

    公开(公告)号：US20110130558A1

    公开(公告)日：2011-06-02

    申请号：US12995348

    申请日：2009-05-12

    申请人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    发明人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    IPC分类号： C07H1/06 ,  C09K3/00

    CPC分类号： C07H1/08 ,  C12N15/1006

    摘要： The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧15% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood

    摘要翻译： 本发明涉及用于从含有起始物质的核酸分离和/或纯化核酸，特别是短链核酸的方法和试剂盒，其特征在于以下方法步骤：（a）将核酸结合至 核酸结合载体材料，其中原料在至少一种离液序列化合物和优选异丙醇的存在下与核酸键合载体材料接触，其中异丙醇以≥15％（v / v）的浓度存在 ）和＆lt; nlE; 35％（v / v），（b）从核酸粘合载体材料任选洗脱键合的核酸。 所述方法特别适用于从母体血液中纯化胎儿DNA