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公开(公告)号：US20130231460A1
公开(公告)日：2013-09-05
申请号：US13821509
申请日：2011-09-07
申请人： Christoph Erbacher , Vera Holländer , Markus Müller , Martin Schlumpberger , Marion Schmidt , Markus Sprenger-Haussels
发明人： Christoph Erbacher , Vera Holländer , Markus Müller , Martin Schlumpberger , Marion Schmidt , Markus Sprenger-Haussels
CPC分类号： G01N1/405 , B01L3/5023 , B01L3/5029 , B01L2300/043 , B01L2300/0609 , C12Q1/6806
摘要： The present invention relates to a method for concentrating one or more target compounds in a liquid sample, a device for carrying out this method and a kit for processing a biological sample comprising such a device.
摘要翻译： 本发明涉及浓缩液体样品中一种或多种目标化合物的方法，用于实施该方法的装置和用于处理包含这种装置的生物样品的试剂盒。
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2.发明授权公开(公告)号：US11542494B2
公开(公告)日：2023-01-03
申请号：US13820228
申请日：2011-09-02
摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.
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3.发明申请METHOD FOR ISOLATING A TARGET NUCLEIC ACID INCLUDING SMALL TARGET NUCLEIC ACIDS WITH HIGH YIELD 审中-公开用于分离靶向核酸的方法，包括具有高YIELD的小目标核酸公开(公告)号：US20130164825A1
公开(公告)日：2013-06-27
申请号：US13820228
申请日：2011-09-02
IPC分类号： C07H1/08
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.
摘要翻译： 本发明涉及从样品中分离包含小靶核酸的靶核酸的方法，所述方法至少包括以下步骤：a）将包含小靶核酸的靶核酸的至少一部分结合至核酸 通过使样品通过所述柱而包含在柱中的酸结合固相，b）对核酸结合固相进行酶和/或化学处理，同时靶核酸与所述固相结合，c）至少收集 在步骤b）的所述处理期间从固相中释放的一部分小目标核酸作为流通，d）使所述流通接触，所述流过包含与回收溶液混合的小目标核酸与核酸结合固相 用于将所含的小靶核酸与所述核酸结合固相结合，e）任选地进行洗脱。 本发明导致分离的靶核酸中小靶核酸的产量显着增加，因为其允许有效捕获和回收小靶核酸。
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公开(公告)号：US20130105393A1
公开(公告)日：2013-05-02
申请号：US13810157
申请日：2011-07-13
IPC分类号： G01N1/40
CPC分类号： G01N1/40 , G01N1/4005 , Y10T29/49
摘要： The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside or at the end of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, the use of such a device for collection, storage, treatment or isolation of a biomolecule or biomolecules containing samples, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device.
摘要翻译： 本发明涉及一种装置，其包括中空体，该中空体具有至少一个开口端和在中空体的内部或端部处的至少一个阻挡物，其在环境条件下对于液体和固体是不可渗透的，然而，变成液体 通过对所述屏障施加压力，牵引力或驱动力等外力，使用这种用于收集，储存，处理或分离生物分子或含有样品的生物分子的装置，所述装置的制备方法和 使用所述装置分离或纯化任何生物分子的方法。
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公开(公告)号：US10094749B2
公开(公告)日：2018-10-09
申请号：US13810157
申请日：2011-07-13
IPC分类号： G01N1/40
摘要： The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside or at the end of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, the use of such a device for collection, storage, treatment or isolation of a biomolecule or biomolecules containing samples, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device.
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公开(公告)号：US10514330B2
公开(公告)日：2019-12-24
申请号：US13809563
申请日：2011-07-13
申请人： Vera Holländer , Markus Müller , Karin Schulte
发明人： Vera Holländer , Markus Müller , Karin Schulte
IPC分类号： G01N1/40
摘要： The present invention refers to a device, comprising a hollow body having at least one open end comprising at least one solid matrix binding, adsorbing, absorbing, chelating or retaining compounds which are not desired in a sample and preferably at least one barrier which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force to the barrier, the use of such a device for isolating or purifying a biomolecule from a sample, a method for preparation of the device and a method for isolation or purification of any biomolecule using said device.
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公开(公告)号：US20130115693A1
公开(公告)日：2013-05-09
申请号：US13809563
申请日：2011-07-13
申请人： Vera Holländer , Markus Müller , Karin Schulte
发明人： Vera Holländer , Markus Müller , Karin Schulte
CPC分类号： G01N1/4005
摘要： The present invention refers to a device, comprising a hollow body having at least one open end comprising at least one solid matrix binding, adsorbing, absorbing, chelating or retaining compounds which are not desired in a sample and preferably at least one barrier which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force to said barrier, the use of such a device for isolating or purifying a biomolecule from a sample, a method for preparation of said device and a method for isolation or purification of any biomolecule using said device.
摘要翻译： 本发明涉及一种装置，其包括中空体，该中空体具有至少一个开放端，该至少一个开放端包含至少一个固体基质结合，吸附，吸收，螯合或保留在样品中不需要的化合物，优选至少一个不是 然而，在环境条件下对于液体和固体是不透气的，通过向所述阻挡层施加外力，使用这种用于从样品中分离或纯化生物分子的装置，所述装置的制备方法和 使用所述装置分离或纯化任何生物分子的方法。
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8.发明申请公开(公告)号：US20110244468A1
公开(公告)日：2011-10-06
申请号：US13132588
申请日：2009-12-04
申请人： Vera Holländer , Peter Porschewski
发明人： Vera Holländer , Peter Porschewski
IPC分类号： C12Q1/68 , G01N1/28 , G01N33/68 , G01N27/447 , C25B7/00
CPC分类号： C12N15/1003 , G01N33/5308 , G01N33/68
摘要： The present invention relates to a method of isolating/extracting in parallel various biomolecules, in particular nucleic acids and proteins, from the same fixed biological samples, to the quantification and analysis of the biomolecules isolated by the method of the invention, and to a kit for isolating/extracting in parallel various biomolecules from a fixed sample, to the use of said kit for diagnosing, prognosing, deciding the therapy of and monitoring the therapy of a disease.
摘要翻译： 本发明涉及将来自相同固定生物样品的各种生物分子，特别是核酸和蛋白质并行分离/提取到通过本发明方法分离的生物分子的定量和分析的方法，以及试剂盒 用于从固定样品中并行分离/提取各种生物分子，使用所述试剂盒来诊断，预测，决定治疗和监测疾病的治疗。
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公开(公告)号：US20100009349A1
公开(公告)日：2010-01-14
申请号：US12087167
申请日：2006-12-29
申请人： Vera Holländer
发明人： Vera Holländer
CPC分类号： B01L3/508 , B01L2300/046 , G01N1/30 , G01N1/31 , Y10T436/107497 , Y10T436/108331 , Y10T436/2525
摘要： The invention relates to a method for treating a biological sample, comprising the following method steps: i) preparation of a biological sample and ii) bringing the biological sample into contact with a composition, comprising: (1) 1 to 100 wt. % of at least one polyol and (2) 0 to 99 wt. % of at least one additive, wherein the total amount of components (1) and (2) is 100 wt. %. The invention further relates to biological samples obtained by said method, a method for analysis of a treated biological sample, devices for treating a biological sample, use of said devices, various kits and use of a composition.
摘要翻译： 本发明涉及一种用于处理生物样品的方法，包括以下方法步骤：i）制备生物样品，和ii）使生物样品与组合物接触，包括：（1）1至100重量％ ％的至少一种多元醇和（2）0〜99重量％ ％的至少一种添加剂，其中组分（1）和（2）的总量为100重量％。 ％。 本发明还涉及通过所述方法获得的生物样品，用于分析经处理的生物样品的方法，用于处理生物样品的装置，所述装置的使用，各种试剂盒和组合物的用途。
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公开(公告)号：US20100255524A1
公开(公告)日：2010-10-07
申请号：US12602142
申请日：2008-05-29
申请人： Vera Holländer
发明人： Vera Holländer
IPC分类号： C12Q1/02 , C07H21/00 , C07K14/00 , C12N5/071 , C12N5/04 , C12N1/16 , C12N7/00 , C12N1/20 , G01N33/48 , G01N33/92 , G01N33/50
CPC分类号： C12Q1/6806 , C12N15/1003 , Y10T436/142222 , C12Q2527/125
摘要： The present invention relates to a method of stabilizing a biological sample, having the steps of preparation of a biological sample, and of contacting the biological sample with a composition, having a substance according to the following structural formula: in which R1 is a hydrogen residue or a methyl residue, R2 and R3 are identical or different hydrocarbon residues with a length of the carbon chain of 1-20, and R4 is an oxygen, sulfur or selenium residue (FIG. 1).
摘要翻译： 本发明涉及一种稳定生物样品的方法，具有制备生物样品的步骤，以及将生物样品与具有根据以下结构式的物质的组合物接触：其中R1是氢残基 或甲基残基，R2和R3是碳链长度为1-20的相同或不同的烃残基，R4是氧，硫或硒残基（图1）。
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