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    Methods and kit for isolating nucleic acids
    2.
    发明授权
    Methods and kit for isolating nucleic acids 失效
    分离核酸的方法和试剂盒

    公开(公告)号：US08206990B2

    公开(公告)日：2012-06-26

    申请号：US12439396

    申请日：2007-10-10

    申请人： Christoph Ritt ,  Christoph Erbacher ,  Patrick Baumhof

    发明人： Christoph Ritt ,  Christoph Erbacher ,  Patrick Baumhof

    IPC分类号： G01N30/56

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  Y10T436/143333 ,  Y10T436/255

    摘要： The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention.

    摘要翻译： 本发明涉及从包含所述靶核酸的样品中分离靶核酸的方法，包括以下步骤：将含有所述靶核酸的样品与结合溶液和核酸结合基质混合，将至少部分 的所述靶核酸与所述核酸结合基质反应，其中所述核酸结合基质同时被处理或已经用至少一种包含选自主要组13至16的金属的金属的化合物进行处理， 半金属和过渡金属，用于降低非靶核酸污染物，或其中所述核酸结合基质用疏水基团修饰。 此外，本发明的教导提供了相应的试剂盒和试剂。

    Methods and kit for isolating nucleic acids
    6.
    发明授权
    Methods and kit for isolating nucleic acids 失效
    分离核酸的方法和试剂盒

    公开(公告)号：US08460941B2

    公开(公告)日：2013-06-11

    申请号：US13465474

    申请日：2012-05-07

    申请人： Christoph Ritt ,  Christoph Erbacher ,  Patrick Baumhof

    发明人： Christoph Ritt ,  Christoph Erbacher ,  Patrick Baumhof

    IPC分类号： G01N33/00

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  Y10T436/143333 ,  Y10T436/255

    摘要： The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention.

    摘要翻译： 本发明涉及从包含所述靶核酸的样品中分离靶核酸的方法，包括以下步骤：将含有所述靶核酸的样品与结合溶液和核酸结合基质混合，将至少部分 的所述靶核酸与所述核酸结合基质反应，其中所述核酸结合基质同时被处理或已经用至少一种包含选自主要组13至16的金属的金属的化合物进行处理， 半金属和过渡金属，用于降低非靶核酸污染物，或其中所述核酸结合基质用疏水基团修饰。 此外，本发明的教导提供了相应的试剂盒和试剂。

    METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE
    8.
    发明申请
    METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE 审中-公开
    在样品中正常化生物分子的内容物的方法

    公开(公告)号：US20110171656A1

    公开(公告)日：2011-07-14

    申请号：US13063598

    申请日：2009-09-16

    申请人： Christoph Erbacher ,  Peter Grünefeld

    发明人： Christoph Erbacher ,  Peter Grünefeld

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6806 ,  C12Q2545/101 ,  C12Q2523/308

    摘要： The present invention relates to a method for normalizing the contents of biomolecules in a sample, comprising the following steps: a) preparing a reaction vessel with a vessel surface that is functionalized at least in sections—preferably on the inside of the vessel—in such a way that the surface can reversibly bind biomolecules under high salt conditions, b) executing at least one sample preparation step, c) binding biomolecules from the prepared sample to the vessel surface (“binding and normalizing step”) under high salt conditions, d) optionally washing (“washing step”), and e) executing at least one subsequent reaction. The application also relates to a reaction vessel as is used in the above method.

    摘要翻译： 本发明涉及一种用于使样品中生物分子的内容物标准化的方法，包括以下步骤：a）制备具有至少在部分 - 优选在容器内部官能化的容器表面的反应容器 - 在这样的 表面可以在高盐条件下可逆地结合生物分子的方式，b）执行至少一个样品制备步骤，c）在高盐条件下将生物分子从制备的样品结合到容器表面（“结合和归一化步骤”），d ）任选地洗涤（“洗涤步骤”），和e）执行至少一个后续反应。 本申请还涉及如上述方法中使用的反应容器。