公开(公告)号：US08124031B2

公开(公告)日：2012-02-28

申请号：US11785431

申请日：2007-04-17

申请人： Tohru Harada ,  Akihiko Yabuki ,  Kiyoshi Taninaka

发明人： Tohru Harada ,  Akihiko Yabuki ,  Kiyoshi Taninaka

IPC分类号： B01L3/02

CPC分类号： C12M35/00 ,  Y10T137/0396 ,  Y10T137/0971 ,  Y10T137/3115 ,  Y10T137/85978

摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.

公开(公告)号：US20080079376A1

公开(公告)日：2008-04-03

申请号：US11785431

申请日：2007-04-17

申请人： Tohru Harada ,  Akihiko Yabuki ,  Kiyoshi Taninaka

发明人： Tohru Harada ,  Akihiko Yabuki ,  Kiyoshi Taninaka

IPC分类号： H02P7/00

CPC分类号： C12M35/00 ,  Y10T137/0396 ,  Y10T137/0971 ,  Y10T137/3115 ,  Y10T137/85978

摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.

摘要翻译： 打开第二阀，将注射压力产生压力与保持压力组合成注射压力。 在向毛细管施加注射压力以使物体排出之后，将调节器的输出压力设定为注射压力。 然后，打开第一阀，以将注射压力重新应用于毛细管。 第二阀打开，将维持压力产生压力与喷射压力组合成维持压力。 在对毛细管施加维持压力以终止物体的排出之后，将调节器的输出压力设定为保持压力。 然后，打开第一个阀，以将保持压力重新应用到毛细管。

公开(公告)号：US08241590B2

公开(公告)日：2012-08-14

申请号：US13354808

申请日：2012-01-20

申请人： Tohru Harada ,  Akihiko Yabuki ,  Kiyoshi Taninaka

发明人： Tohru Harada ,  Akihiko Yabuki ,  Kiyoshi Taninaka

IPC分类号： B01L3/02

CPC分类号： C12M35/00 ,  Y10T137/0396 ,  Y10T137/0971 ,  Y10T137/3115 ,  Y10T137/85978

摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.

摘要翻译： 打开第二阀，将注射压力产生压力与保持压力组合成注射压力。 在向毛细管施加注射压力以使物体排出之后，将调节器的输出压力设定为注射压力。 然后，打开第一阀，以将注射压力重新应用于毛细管。 第二阀打开，将维持压力产生压力与喷射压力组合成维持压力。 在对毛细管施加维持压力以终止物体的排出之后，将调节器的输出压力设定为保持压力。 然后，打开第一个阀，以将保持压力重新应用到毛细管。

公开(公告)号：US20120111886A1

公开(公告)日：2012-05-10

申请号：US13354808

申请日：2012-01-20

申请人： Tohru HARADA ,  Akihiko Yabuki ,  Kiyoshi Taninaka

发明人： Tohru HARADA ,  Akihiko Yabuki ,  Kiyoshi Taninaka

IPC分类号： B67D7/08

CPC分类号： C12M35/00 ,  Y10T137/0396 ,  Y10T137/0971 ,  Y10T137/3115 ,  Y10T137/85978

摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.

摘要翻译： 打开第二阀，将注射压力产生压力与保持压力组合成注射压力。 在向毛细管施加注射压力以使物体排出之后，将调节器的输出压力设定为注射压力。 然后，打开第一阀，以将注射压力重新应用于毛细管。 第二阀打开，将维持压力产生压力与喷射压力组合成维持压力。 在对毛细管施加维持压力以终止物体的排出之后，将调节器的输出压力设定为保持压力。 然后，打开第一个阀，以将保持压力重新应用到毛细管。

公开(公告)号：US08859224B2

公开(公告)日：2014-10-14

申请号：US11785410

申请日：2007-04-17

申请人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

发明人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

IPC分类号： C12Q1/02 ,  C12M1/42 ,  C12M1/34

CPC分类号： C12M35/00 ,  C12M41/00 ,  C12M41/40 ,  C12M41/44

摘要： A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.

摘要翻译： 荧光强度检测单元通过将含有荧光试剂的第一溶液通过注射部件注入到与第一溶液不形成界面的第二溶液中来检测荧光强度。 计算单元基于荧光强度和预先测量的注射量之间的相关性，从荧光强度计算第一溶液的注射量。 计算单元基于所计算的喷射量来获得喷射量，压力和加压时间之间的相关性。 调整单元通过基于获得的相关性来控制压力和加压时间来调节要注入内质网的第一溶液的量。

公开(公告)号：US08012417B2

公开(公告)日：2011-09-06

申请号：US11797630

申请日：2007-05-04

申请人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

发明人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

IPC分类号： B01L3/02

CPC分类号： C12N15/89 ,  C12M35/00 ,  C12M41/12 ,  G01N35/1016 ,  G01N35/1095 ,  Y10T137/218 ,  Y10T137/2218

摘要： A microinjection device includes a pressure pump and a regulator that is connected to the pressure pump and that maintains constant pressure. A regulating chamber is connected to the regulator and an internal pressure of the regulating chamber is maintained to a predetermined pressure. A valve is connected to the regulating chamber and a hollow capillary is connected to the valve. An operator opens/closes the valve in the process of injecting material in a cell.

摘要翻译： 显微注射装置包括压力泵和调节器，其连接到压力泵并保持恒定的压力。 调节室连接到调节器，并且调节室的内部压力保持在预定压力。 阀连接到调节室，中空毛细管连接到阀。 操作者在将电池注入材料的过程中打开/关闭阀门。

公开(公告)号：US20080206802A1

公开(公告)日：2008-08-28

申请号：US11785410

申请日：2007-04-17

申请人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

发明人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

IPC分类号： C12Q1/02 ,  C12M1/36

CPC分类号： C12M35/00 ,  C12M41/00 ,  C12M41/40 ,  C12M41/44

摘要： A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.

摘要翻译： 荧光强度检测单元通过将含有荧光试剂的第一溶液通过注射部件注入到与第一溶液不形成界面的第二溶液中来检测荧光强度。 计算单元基于荧光强度和预先测量的注射量之间的相关性，从荧光强度计算第一溶液的注射量。 计算单元基于所计算的喷射量来获得喷射量，压力和加压时间之间的相关性。 调整单元通过基于获得的相关性来控制压力和加压时间来调节要注入内质网的第一溶液的量。

公开(公告)号：US20070264162A1

公开(公告)日：2007-11-15

申请号：US11797630

申请日：2007-05-04

申请人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

发明人： Kiyoshi Taninaka ,  Jun Sasaki ,  Akihiko Yabuki

IPC分类号： B01L3/02

CPC分类号： C12N15/89 ,  C12M35/00 ,  C12M41/12 ,  G01N35/1016 ,  G01N35/1095 ,  Y10T137/218 ,  Y10T137/2218

摘要： A microinjection device includes a pressure-pump and a regulator that is connected to the pressure pump and that maintains constant pressure. A regulating chamber is connected to the regulator and an internal pressure of the regulating chamber is maintained to a predetermined pressure. A valve is connected to the regulating chamber and a hollow capillary is connected to the valve. An operator opens/closes the valve in the process of injecting material in a cell.

摘要翻译： 显微注射装置包括压力泵和调节器，其连接到压力泵并保持恒定的压力。 调节室连接到调节器，并且调节室的内部压力保持在预定压力。 阀连接到调节室，中空毛细管连接到阀。 操作者在将电池注入材料的过程中打开/关闭阀门。

公开(公告)号：US20060246571A1

公开(公告)日：2006-11-02

申请号：US11117771

申请日：2005-04-29

申请人： Sachihiro Youoku ,  Jun Sasaki ,  Junichi Murakoso ,  Nobuhiko Onda ,  Kazuo Tamamushi ,  Kiyoshi Taninaka

发明人： Sachihiro Youoku ,  Jun Sasaki ,  Junichi Murakoso ,  Nobuhiko Onda ,  Kazuo Tamamushi ,  Kiyoshi Taninaka

IPC分类号： C12N15/00

CPC分类号： C12M35/00

摘要： Proposed is an apparatus for automatically producing particles having introduced therein a substance. The apparatus includes a feeding device that feeds a suspension containing a plurality of particles; a holding device that receives the suspension from the feeding device and immobilizes and release at least one particle reversibly; an injecting device that injects a substance into the particle while the particle has been immobilized by the holding device; and a removing device that removes particles that are not immobilized by the holding device.

摘要翻译： 提出了一种用于自动生成在其中引入物质的颗粒的装置。 该装置包括供给装置，其供给包含多个颗粒的悬浮液; 保持装置，其从所述供给装置接收所述悬浮液，并且可逆地固定和释放至少一个颗粒; 注射装置，其中通过所述保持装置固定所述颗粒，将物质注入所述颗粒中; 以及去除未被固定装置固定的颗粒的去除装置。

公开(公告)号：US5835822A

公开(公告)日：1998-11-10

申请号：US678746

申请日：1996-07-11

申请人： Yoshiki Nagasaki ,  Michio Shimura ,  Tetsuya Takei ,  Kiyoshi Taninaka ,  Nobuo Kuwabara ,  Morihisa Kawahara ,  Minoru Takano ,  Hiroyuki Kawai ,  Kunihiro Kitsu ,  Junji Wakihara

发明人： Yoshiki Nagasaki ,  Michio Shimura ,  Tetsuya Takei ,  Kiyoshi Taninaka ,  Nobuo Kuwabara ,  Morihisa Kawahara ,  Minoru Takano ,  Hiroyuki Kawai ,  Kunihiro Kitsu ,  Junji Wakihara

IPC分类号： G03G15/09 ,  B41J2/43 ,  B65H1/08 ,  B65H3/06 ,  B65H3/52 ,  B65H9/10 ,  G03G15/00 ,  G03G15/02 ,  G03G15/08 ,  G03G15/20 ,  G03G21/10 ,  G03G21/18

CPC分类号： G03G21/1821 ,  B41J2/43 ,  B65H1/08 ,  B65H3/0669 ,  B65H3/5223 ,  B65H9/106 ,  G03G15/0291 ,  G03G15/0855 ,  G03G15/0865 ,  G03G15/0877 ,  G03G15/0893 ,  G03G15/2003 ,  G03G15/6511 ,  G03G15/80 ,  G03G21/105 ,  B65H2511/51 ,  G03G2215/00396 ,  G03G2215/00405 ,  G03G2215/00561 ,  G03G2215/00721 ,  G03G2221/1621 ,  G03G2221/163 ,  G03G2221/1693

摘要： An electrophotographic image forming apparatus includes an electrostatic latent image carrying body, an electric charger for uniformly electrifying the carrying body, an optical exposure unit to form an electrostatic latent image on the carrying body, a developer for developing the electrostatic latent image to form a toner image on the carrying body, a transfer unit for transferring the toner image from the carrying body onto a printing sheet, a fixing unit for fixing the toner image to the printing sheet, a cleaner for cleaning to remove residual toner from the carrying body and a toner returning mechanism for returning the residual toner removed by the cleaner to the developer. The developer includes a housing in which a toner chamber is defined, a developing roller rotatably mounted in the housing, an agitator rotatably mounted in the housing so as to be positioned in the toner chamber, a partitioning member provided in the housing for defining a carrier chamber adjacent to the developing roller. The toner returning mechanism includes a toner recirculator for recirculating the residual toner removed from the carrying body to the toner chamber.

摘要翻译： 电子照相图像形成装置包括静电潜像携带体，用于使携带体均匀带电的充电器，在载体上形成静电潜像的光学曝光单元，用于使静电潜像显影以形成调色剂的显影剂 载体上的图像，用于将调色剂图像从载体转印到打印片材上的转印单元，用于将调色剂图像定影到打印片材的定影单元，用于清洁以从载体中除去残留调色剂的清洁剂和 用于将由清洁剂除去的残留调色剂返回到显影剂的调色剂返回机构。 显影器包括：壳体，其中限定了调色剂室，可旋转地安装在壳体中的显影辊，可旋转地安装在壳体中以便定位在调色剂室中的搅拌器，设置在壳体中用于限定载体的分隔构件 与显影辊相邻。 调色剂返回机构包括调色剂再循环器，用于将从承载体移除的残余调色剂再循环到调色剂室。