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公开(公告)号：US09193963B2
公开(公告)日：2015-11-24
申请号：US14195923
申请日：2014-03-04
发明人： Hiroyuki Kurihara , Takeshi Tsukada , Katsushige Yamada , Yumiko Mishima , Yuka Maeno , Kazuhiko Ishikawa
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C13K1/02
摘要： Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.
摘要翻译： 内切葡聚糖酶的特征在于木质素衍生的芳族化合物的活性抑制程度降低，并且通过用野生型嗜热细菌来源的内切葡聚糖酶的氨基酸序列中的位置273处的色氨酸与除芳香族氨基酸以外的氨基酸取代。
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公开(公告)号：US20140178947A1
公开(公告)日：2014-06-26
申请号：US14195923
申请日：2014-03-04
发明人： Hiroyuki Kurihara , Takeshi Tsukada , Katsushige Yamada , Yumiko Mishima , Yuka Maeno , Kazuhiko Ishikawa
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C13K1/02
摘要： Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.
摘要翻译： 内切葡聚糖酶的特征在于木质素衍生的芳族化合物的活性抑制程度降低，并且通过用野生型嗜热细菌来源的内切葡聚糖酶的氨基酸序列中的位置273处的色氨酸与除芳香族氨基酸以外的氨基酸取代。
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3.发明授权Method for producing glucosidase, enzyme composition, and method for hydrolyzing biomass 有权生产葡萄糖苷酶的方法，酶组成和水解生物量的方法公开(公告)号：US09034627B2
公开(公告)日：2015-05-19
申请号：US13772533
申请日：2013-02-21
发明人： Hiroyuki Kurihara , Shiomi Watanabe , Katsushige Yamada , Kazuhiko Ishikawa , Yasunobu Wada , Yuji Kado
CPC分类号： C12N9/2437 , C12N9/2445 , C12P21/005 , C12Y302/01021
摘要： A method for producing a mutant glucosidase includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. An enzyme composition contains the mutant glucosidase.
摘要翻译： 制备突变型葡糖苷酶的方法包括将编码分泌信号序列的DNA和编码Asn-X-Ser或Asn-X-Thr的DNA引入编码嗜热菌的葡糖苷酶的DNA中，并将得到的DNA导入真核生物微生物并表达 它作为分泌蛋白。 酶组合物含有突变型葡糖苷酶。
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4.发明申请MUTANT B-GLUCOSIDASE, ENZYME COMPOSITION FOR DECOMPOSING BIOMASS, AND METHOD OF PRODUCING SUGAR SOLUTION 有权MUTANT B-GLUCOSIDASE，用于分解生物量的酶组合物和生产糖溶液的方法公开(公告)号：US20140127759A1
公开(公告)日：2014-05-08
申请号：US14154547
申请日：2014-01-14
发明人： Hiroyuki Kurihara , Katsushige Yamada , Kazuhiko Ishikawa , Yumiko Mishima , Yasunobu Wada , Yuji Kado
CPC分类号： C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： A β-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The β-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent β-glucosidase with other amino acids and exhibits a decomposition activity.
摘要翻译： 葡萄糖苷酶在生物质存在下表现出高活性，与常规酶相比具有高热稳定性。 β-葡糖苷酶包括氨基酸的取代和/或缺失，至少三种氨基酸，其选自亲本＆amp;葡糖苷酶与其它氨基酸的Glu39，Asp169，Arg170，Arg220，Tyr227和Glu330 并表现出分解活性。
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公开(公告)号：US10435728B2
公开(公告)日：2019-10-08
申请号：US15521654
申请日：2015-11-05
发明人： Hiroyuki Kurihara , Takuya Kasahara , Chiaki Yamada , Natsuko Murakami , Katsushige Yamada , Kazuhiko Ishikawa , Masahiro Watanabe
摘要： An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.
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6.发明申请公开(公告)号：US20170240941A1
公开(公告)日：2017-08-24
申请号：US15521654
申请日：2015-11-05
发明人： Hiroyuki Kurihara , Takuya Kasahara , Chiaki Yamada , Natsuko Murakami , Katsushige Yamada , Kazuhiko Ishikawa , Masahiro Watanabe
CPC分类号： C12P19/14 , C12N5/00 , C12N9/2482 , C12N15/09 , C12P19/02 , C12Y302/01008
摘要： An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.
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7.发明授权Mutant β-glucosidase, enzyme composition for decomposing biomass, and method of producing sugar solution 有权突变体 - 葡萄糖苷酶，用于分解生物质的酶组合物和产生糖溶液的方法公开(公告)号：US09080164B2
公开(公告)日：2015-07-14
申请号：US14154547
申请日：2014-01-14
发明人： Hiroyuki Kurihara , Katsushige Yamada , Kazuhiko Ishikawa , Yumiko Mishima , Yasunobu Wada , Yuji Kado
CPC分类号： C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： A β-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The β-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent β-glucosidase with other amino acids and exhibits a decomposition activity.
摘要翻译： 葡萄糖苷酶在生物质存在下表现出高活性，与常规酶相比具有高热稳定性。 β-葡糖苷酶包括氨基酸的取代和/或缺失，至少三种氨基酸，其选自亲本＆amp;葡糖苷酶与其它氨基酸的Glu39，Asp169，Arg170，Arg220，Tyr227和Glu330 并表现出分解活性。
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8.发明申请METHOD FOR PRODUCING GLUCOSIDASE, ENZYME COMPOSITION, AND METHOD FOR HYDROLYZING BIOMASS 有权生产葡萄球菌素的方法，酶组合物和水解生物量的方法公开(公告)号：US20130189761A1
公开(公告)日：2013-07-25
申请号：US13772533
申请日：2013-02-21
发明人： Hiroyuki Kurihara , Shiomi Watanabe , Katsushige Yamada , Kazuhiko Ishikawa , Yasunobu Wada , Yuji Kado
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/2445 , C12P21/005 , C12Y302/01021
摘要： A method for producing a mutant glucosidase includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. An enzyme composition contains the mutant glucosidase.
摘要翻译： 制备突变型葡糖苷酶的方法包括将编码分泌信号序列的DNA和编码Asn-X-Ser或Asn-X-Thr的DNA引入编码嗜热菌的葡糖苷酶的DNA中，并将得到的DNA导入真核生物微生物并表达 它作为分泌蛋白。 酶组合物含有突变型葡糖苷酶。
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