利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

38 条记录 (耗时 0.033 秒)

    Modified thermostable DNA polymerase
    1.
    发明授权
    Modified thermostable DNA polymerase 有权
    修饰的热稳定DNA聚合酶

    公开(公告)号：US07422882B2

    公开(公告)日：2008-09-09

    申请号：US09852922

    申请日：2001-05-10

    申请人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1252

    摘要： An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.

    摘要翻译： 本发明的目的是提供具有增强的扩增效率和/或聚合酶链反应（PCR）中的改进的保真度的热稳定性DNA聚合酶，并提供其生产方法。 更具体地说，本发明提供了热稳定的DNA聚合酶，其中在X 1，X 2，X 3，X 4， 序列（D：天冬氨酸，E：谷氨酸，H：组氨酸，X 1，X 2，X 3和X 3， 由SEQ ID NO：1区域内的DX1E序列组成的4个氨基酸长度肽与邻近所述谷氨酸（E）的4个氨基酸长度的肽组成，所述热稳定性DNA聚合酶具有3' -5'核酸外切酶活性，组氨酸（H）已被另一种氨基酸取代。

    Dna synthesis promoters dna polymerase-associated factors and utilization thereof
    2.
    发明申请
    Dna synthesis promoters dna polymerase-associated factors and utilization thereof 有权
    Dna合成启动子dna聚合酶相关因子及其利用

    公开(公告)号：US20050069887A1

    公开(公告)日：2005-03-31

    申请号：US10495581

    申请日：2002-11-14

    申请人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C07K14/195 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C07K14/195 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2521/101

    摘要： A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy  (1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3. The present invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or bind to DNA polymerase.

    摘要翻译： 一种用于增强DNA合成的组合物，其包含在促进DNA合成反应中有效促进DNA合成的阴离子供给物质，特别是二羧酸的盐。 通过与阴离子供给物质一起使用至少一种选自二甲基亚砜和下式（1）表示的化合物的化合物，可以实现进一步增强的DNA合成促进效果：R 2 -CH 2 -NR <1> xHy（1）其中R 1是具有1至3个碳原子的烷基，R 2是选自以下（a）和（b）的取代基：（a）氧 和（b）由下式表示的基团其中R 4是甲基，氢或当与R 1一起形成吡咯烷环时，R 5是-CO 2 H或-SO 3 H，n是0至 2，x是1至3的整数，y是0至2的整数，条件是x加y等于3.本发明还提供了源自Thermococcus物种的热稳定DNA聚合酶相关因子，其具有活性 促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合。

    RNA polymerase mutants with increased thermostability
    4.
    发明授权
    RNA polymerase mutants with increased thermostability 有权
    具有增加的热稳定性的RNA聚合酶突变体

    公开(公告)号：US07507567B2

    公开(公告)日：2009-03-24

    申请号：US10220908

    申请日：2001-03-01

    申请人： Akio Sugiyama ,  Yoshiaki Nishiya ,  Bunsei Kawakami

    发明人： Akio Sugiyama ,  Yoshiaki Nishiya ,  Bunsei Kawakami

    IPC分类号： C12N9/12 ,  C12N15/11 ,  C12N1/20 ,  C12P19/34

    CPC分类号： C12N9/1247 ,  C12Y207/07006

    摘要： The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.

    摘要翻译： 本申请涉及来自具有增加的稳定性的噬菌体的突变的RNA聚合酶，例如在高温条件下。 根据本发明的优选的突变型RNA聚合酶是来自T7或SP3噬菌体的突变型RNA聚合酶。 本发明的特别优选的实施方案是具有丝氨酸以在氨基酸序列的633位的蛋白质中脯氨酸变化的T7RNA聚合酶。

    NOVEL GLUCOSE DEHYDROGENASE
    5.
    发明申请
    NOVEL GLUCOSE DEHYDROGENASE 有权
    新葡萄糖脱氢酶

    公开(公告)号：US20110020851A1

    公开(公告)日：2011-01-27

    申请号：US12811735

    申请日：2008-12-26

    申请人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    发明人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    IPC分类号： C12Q1/54 ,  C12N9/04 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12Y101/05002 ,  C12N9/0006 ,  C12Q1/32 ,  C12Q1/54

    摘要： The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80° C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.

    摘要翻译： 本发明提供了一种葡萄糖脱氢酶，其是具有80℃或更高的热稳定性的非常稳定的酶，并且基本上不对葡萄糖以外的糖起作用（例如，相对于麦芽糖具有小于3％的反应性， 半乳糖和木糖）。 本发明还提供了生产这种酶的方法，以及使用这种酶定量葡萄糖的组合物。

    Glucose dehydrogenase
    6.
    发明授权
    Glucose dehydrogenase 有权
    葡萄糖脱氢酶

    公开(公告)号：US08394615B2

    公开(公告)日：2013-03-12

    申请号：US12811735

    申请日：2008-12-26

    申请人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    发明人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/54 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12Y101/05002 ,  C12N9/0006 ,  C12Q1/32 ,  C12Q1/54

    摘要： The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80° C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.

    摘要翻译： 本发明提供了一种葡萄糖脱氢酶，其是具有80℃或更高的热稳定性的非常稳定的酶，并且基本上不对葡萄糖以外的糖起作用（例如，相对于麦芽糖具有小于3％的反应性， 半乳糖和木糖）。 本发明还提供了生产这种酶的方法，以及使用这种酶定量葡萄糖的组合物。

    Modified flavin adenine dinucleotide dependent glucose dehydrogenase
    7.
    发明授权
    Modified flavin adenine dinucleotide dependent glucose dehydrogenase 有权
    改良黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶

    公开(公告)号：US07662600B2

    公开(公告)日：2010-02-16

    申请号：US11939348

    申请日：2007-11-13

    申请人： Hiroshi Kawaminami ,  Yuji Tsuji ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    发明人： Hiroshi Kawaminami ,  Yuji Tsuji ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    IPC分类号： C12N9/04 ,  C12Q1/54 ,  C12P19/34 ,  C12N15/00 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12N9/0006

    摘要： An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

    摘要翻译： 本发明的目的是提供比用作血糖传感器的已知酶更可实用的用作测量血糖的试剂的酶。 与来自野生型FADGDH的FADGDH相比，具有比来自野生型FADGDH的FADGDH更好的热稳定性的改良的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FADGDH），修饰的FADGDH优选来自真核生物，更优选丝状真菌，更优选来自真菌， 例如具有至少一个氨基酸被取代，缺失，插入或添加到具有序列表中SEQ ID No.2或46所示的氨基酸序列的FADGDH的一级结构的那些。

    METHOD FOR ENHANCING STABILITY OF A COMPOSITION COMPRISING SOLUBLE GLUCOSE DEHYDROGENASE (GDH)
    9.
    发明申请
    METHOD FOR ENHANCING STABILITY OF A COMPOSITION COMPRISING SOLUBLE GLUCOSE DEHYDROGENASE (GDH) 审中-公开
    包含可溶葡萄糖脱氢酶（GDH）的组合物稳定性的方法

    公开(公告)号：US20080003628A1

    公开(公告)日：2008-01-03

    申请号：US11694489

    申请日：2007-03-30

    申请人： Masao Kitabayashi ,  Yuji Tsuji ,  Yoshiaki Nishiya ,  Takahide Kishimoto

    发明人： Masao Kitabayashi ,  Yuji Tsuji ,  Yoshiaki Nishiya ,  Takahide Kishimoto

    IPC分类号： C12N9/96 ,  C12Q1/54

    CPC分类号： C12N9/0006 ,  C12N9/96 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/3271

    摘要： The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

    摘要翻译： 本发明涉及提高包含可溶性葡萄糖脱氢酶（GDH）的组合物稳定性的方法。 可溶性GDH优选来自丝状真菌的FAD依赖性GDH，并且在来自A.trreus的米曲霉或FAD-GDH的FAD-GDH中观察到最好的效果。 根据本发明，在包含可溶性葡萄糖脱氢酶（GDH）的组合物中，可以通过将酶与一种或多种选自不是酶底物的氨基酸和糖类的化合物共存来增强GDH的稳定性，从而预期增强 葡萄糖测量精度。