公开(公告)号：US12077796B2

公开(公告)日：2024-09-03

申请号：US16615419

申请日：2018-05-29

Applicant: UCB BIOPHARMA SRL

Inventor： Bassem Ben Yahia ,  Laetitia Malphettes ,  Nadine Kochanowski ,  Gill Renner ,  Sandrine Durran ,  Andrew Jeffrey Yates

IPC: C12P21/02 ,  C07K1/16 ,  C07K16/00 ,  C12N5/00

CPC classification number: C12P21/02 ,  C07K1/16 ,  C07K16/00 ,  C12N5/0018 ,  C07K2317/14 ,  C07K2317/565 ,  C12N2500/32 ,  C12N2510/02 ,  C12N2511/00 ,  C12N2523/00

Abstract: The present invention relates to the use of limited amounts of cysteine and tryptophan in the cell culture medium during production of recombinant proteins, and in particular antibodies. Proteins and antibodies produced under such controlled conditions exhibit reduced heterogeneity, in particular reduced charge variants heterogeneity.

公开(公告)号：US11859203B2

公开(公告)日：2024-01-02

申请号：US16768091

申请日：2018-11-29

Applicant: UCB BIOPHARMA SRL

Inventor： Valentine Chevallier ,  Nadine Kochanowski ,  Laetitia Malphettes ,  Vincent Adolphe Carol Cool

IPC: C12N5/00 ,  C07K16/46

CPC classification number: C12N5/0018 ,  C07K16/461 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/515 ,  C12N2500/32

Abstract: The present invention relates to methods for reducing the heterogeneity of a population of recombinant proteins produced in cell culture, said methods comprising growing host cells producing a recombinant protein in a cell culture medium wherein the cell culture medium comprises one or more cysteine/cystine analogs.