公开(公告)号：US20210139980A1

公开(公告)日：2021-05-13

申请号：US17155226

申请日：2021-01-22

Applicant: Ultima Genomics, Inc.

Inventor： Gilad ALMOGY ,  Nathan BECKETT ,  Jacob A. WOLF ,  Nathan CASWELL ,  Joseph ANTHONY ,  Jose Martin SOSA ,  Phillip LEE ,  Stephanie KUBECKA

IPC: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6809 ,  B01J19/00 ,  B01L3/00

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

公开(公告)号：US20240360505A1

公开(公告)日：2024-10-31

申请号：US18650407

申请日：2024-04-30

Applicant: Ultima Genomics, Inc.

Inventor： Nathan BECKETT ,  Gilad ALMOGY ,  Nathan CASWELL ,  Jacob A. WOLF ,  Kristopher BARBEE ,  Denis PRISTINSKI ,  Mark PRATT ,  Gene POLOVY ,  Osip SCHWARTZ ,  Stephanie KUBECKA ,  Steven MENCHEN ,  Joseph ANTHONY ,  Jose Martin SOSA ,  Phillip You Fai LEE

IPC: C12Q1/6874 ,  G01N35/10

CPC classification number: C12Q1/6874 ,  G01N35/1002

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

公开(公告)号：US20240226900A1

公开(公告)日：2024-07-11

申请号：US18391291

申请日：2023-12-20

Applicant: Ultima Genomics, Inc.

Inventor： Jose Martin SOSA ,  Joseph ANTHONY ,  Nathan BECKETT ,  Gilad ALMOGY ,  Chandan SHEE ,  Phillip You Fai LEE ,  Yuto WATANABE

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/686

CPC classification number: B01L7/52 ,  B01L3/508 ,  B01L2200/0647 ,  B01L2300/1805 ,  B01L2400/0415 ,  C12Q1/686

Abstract: The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). Methods and systems provided herein may facilitate performing reactions such as emulsion PCR (ePCR) on samples comprising nucleic acids and beads. The methods provided herein may provide a higher throughput compared to existing technologies.

公开(公告)号：US20210079464A1

公开(公告)日：2021-03-18

申请号：US16953071

申请日：2020-11-19

Applicant: Ultima Genomics, Inc.

Inventor： Nathan BECKETT ,  Gilad ALMOGY ,  Nathan CASWELL ,  Jacob A. WOLF ,  Kristopher BARBEE ,  Denis PRINTINSKI ,  Mark PRATT ,  Gene POLOVY ,  Osip SCHWARTZ ,  Stephanie KUBECKA ,  Steven MENCHEN ,  Joseph ANTHONY ,  Jose Martin SOSA ,  Phillip You Fai LEE

IPC: C12Q1/6874 ,  G01N35/10

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

公开(公告)号：US20200291469A1

公开(公告)日：2020-09-17

申请号：US16445798

申请日：2019-06-19

Applicant: Ultima Genomics, Inc.

Inventor： Gilad ALMOGY ,  Nathan BECKETT ,  Jacob A. WOLF ,  Nathan CASWELL ,  Joseph ANTHONY ,  Jose Martin SOSA ,  Phillip LEE ,  Stephanie KUBECKA

IPC: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6809 ,  B01J19/00 ,  B01L3/00

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

公开(公告)号：US20200179925A1

公开(公告)日：2020-06-11

申请号：US16665540

申请日：2019-10-28

Applicant: Ultima Genomics, Inc.

Inventor： Nathan BECKETT ,  Nathan CASWELL

IPC: B01L3/00 ,  G01N33/487 ,  G01N7/02

Abstract: Provided herein are methods for processing and/or detecting a sample. A method can comprise providing a barrier between a first region and a second region, wherein the first region comprises the sample, wherein the barrier maintains the first region at a first atmosphere that is different than a second atmosphere of the second region, wherein a portion of the barrier comprises a fluid in coherent motion; and using a detector at least partially contained in the first region to detect one or more signals from the sample while the first region is maintained at the first atmosphere that is different than the second atmosphere of the second region. The portion of the barrier comprising fluid may have a pressure lower than the first atmosphere, the second atmosphere, or both.

公开(公告)号：US20230152300A1

公开(公告)日：2023-05-18

申请号：US17962163

申请日：2022-10-07

Applicant: Ultima Genomics, Inc.

Inventor： Kristopher BARBEE ,  Nathan BECKETT ,  Denis PRISTINSKI ,  Derek SCHULTE ,  Avishai BARTOV ,  Jamie SULLIVAN ,  Dumitru BRINZA ,  Abizar LAKDAWALLA ,  Steven MENCHEN ,  Gilad ALMOGY ,  Mark PRATT

IPC: G01N33/49 ,  G01N1/02 ,  G01Q10/04

CPC classification number: G01N33/492 ,  G01N1/02 ,  G01Q10/04 ,  G01N2001/2291

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.

公开(公告)号：US20220168745A1

公开(公告)日：2022-06-02

申请号：US17673444

申请日：2022-02-16

Applicant: Ultima Genomics, Inc.

Inventor： Jose Martin SOSA ,  Joseph ANTHONY ,  Nathan BECKETT ,  Gilad ALMOGY ,  Chandan SHEE ,  Phillip You Fai LEE ,  Yuto WATANABE

IPC: B01L7/00 ,  B01L3/00

Abstract: The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). Methods and systems provided herein may facilitate performing reactions such as emulsion PCR (ePCR) on samples comprising nucleic acids and beads. The methods provided herein may provide a higher throughput compared to existing technologies.

公开(公告)号：US20210277464A1

公开(公告)日：2021-09-09

申请号：US17308241

申请日：2021-05-05

Applicant: Ultima Genomics, Inc.

Inventor： Nathan BECKETT ,  Gilad ALMOGY ,  Nathan CASWELL ,  Jacob A. WOLF ,  Kristopher BARBEE ,  Denis PRISTINSKI ,  Mark PRATT ,  Gene POLOVY ,  Osip SCHWARTZ ,  Stephanie KUBECKA ,  Steven MENCHEN ,  Joseph ANTHONY ,  Jose Martin SOSA ,  Phillip You Fai LEE

IPC: C12Q1/6874 ,  G01N35/10

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

公开(公告)号：US20210054454A1

公开(公告)日：2021-02-25

申请号：US17089781

申请日：2020-11-05

Applicant: Ultima Genomics, Inc.

Inventor： Gilad ALMOGY ,  Kristopher BARBEE ,  Nathan BECKETT

IPC: C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6825 ,  B01J19/00 ,  B01L3/00

Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.