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公开(公告)号：US20240360505A1
公开(公告)日：2024-10-31
申请号：US18650407
申请日：2024-04-30
发明人： Nathan BECKETT , Gilad ALMOGY , Nathan CASWELL , Jacob A. WOLF , Kristopher BARBEE , Denis PRISTINSKI , Mark PRATT , Gene POLOVY , Osip SCHWARTZ , Stephanie KUBECKA , Steven MENCHEN , Joseph ANTHONY , Jose Martin SOSA , Phillip You Fai LEE
IPC分类号： C12Q1/6874 , G01N35/10
CPC分类号： C12Q1/6874 , G01N35/1002
摘要： Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.
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公开(公告)号：US20240263216A1
公开(公告)日：2024-08-08
申请号：US18410793
申请日：2024-01-11
发明人： Linda G. LEE , Gilad ALMOGY , Steven MENCHEN
IPC分类号： C12Q1/6823 , C12Q1/6869 , C12Q1/6876
CPC分类号： C12Q1/6823 , C12Q1/6869 , C12Q1/6876
摘要： The present disclosure provides labeling reagents for labeling substrates such as nucleotides, proteins, antibodies, lipids, and cells. The labeling reagents provided herein may comprise fluorescent labels and semi-rigid linkers. Methods for nucleic acid sequencing using materials comprising such labeling reagents are also provided here.
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公开(公告)号：US20230348969A1
公开(公告)日：2023-11-02
申请号：US18132703
申请日：2023-04-10
发明人： Mark PRATT , Florian OBERSTRASS , Linda LEE , Steven MENCHEN
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Y207/07007 , C12Q2537/157 , C12Q2549/10 , C12Q2537/155 , C12Q2535/113
摘要： The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.
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公开(公告)号：US20210079464A1
公开(公告)日：2021-03-18
申请号：US16953071
申请日：2020-11-19
发明人： Nathan BECKETT , Gilad ALMOGY , Nathan CASWELL , Jacob A. WOLF , Kristopher BARBEE , Denis PRINTINSKI , Mark PRATT , Gene POLOVY , Osip SCHWARTZ , Stephanie KUBECKA , Steven MENCHEN , Joseph ANTHONY , Jose Martin SOSA , Phillip You Fai LEE
IPC分类号： C12Q1/6874 , G01N35/10
摘要： Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.
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公开(公告)号：US20200318170A1
公开(公告)日：2020-10-08
申请号：US16822121
申请日：2020-03-18
发明人： Linda LEE , Steven MENCHEN , Theo NIKIFOROV , Gilad ALMOGY , Florian OBERSTRASS
IPC分类号： C12Q1/6823 , C12Q1/6869 , C12Q1/6806 , G01N21/64
摘要： The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.
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公开(公告)号：US20220162696A1
公开(公告)日：2022-05-26
申请号：US17543521
申请日：2021-12-06
发明人： Nathan BECKETT , Gilad ALMOGY , Nathan CASWELL , Jacob A. WOLF , Kristopher BARBEE , Denis PRISTINSKI , Mark PRATT , Gene POLOVY , Osip SCHWARTZ , Stephanie KUBECKA , Steven MENCHEN , Joseph ANTHONY , Jose Martin SOSA , Phillip You Fai LEE
IPC分类号： C12Q1/6874 , G01N35/10
摘要： Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.
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公开(公告)号：US20210363572A1
公开(公告)日：2021-11-25
申请号：US17395382
申请日：2021-08-05
发明人： Linda G. LEE , Gilad ALMOGY , Steven MENCHEN
IPC分类号： C12Q1/6823 , C12Q1/6876 , C12Q1/6869
摘要： The present disclosure provides labeling reagents for labeling substrates such as nucleotides, proteins, antibodies, lipids, and cells. The labeling reagents provided herein may comprise fluorescent labels and semi-rigid linkers. Methods for nucleic acid sequencing using materials comprising such labeling reagents are also provided here
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公开(公告)号：US20190153520A1
公开(公告)日：2019-05-23
申请号：US15974543
申请日：2018-05-08
发明人： Kristopher BARBEE , Nathan BECKETT , Denis PRISTINSKI , Derek SCHULTE , Avishai BARTOV , Jamie SULLIVAN , Dumitru BRINZA , Abizar LAKDAWALLA , Steven MENCHEN , Gilad ALMOGY , Mark PRATT
IPC分类号： C12Q1/6825 , B01L3/00
摘要： Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.
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公开(公告)号：US20230152300A1
公开(公告)日：2023-05-18
申请号：US17962163
申请日：2022-10-07
发明人： Kristopher BARBEE , Nathan BECKETT , Denis PRISTINSKI , Derek SCHULTE , Avishai BARTOV , Jamie SULLIVAN , Dumitru BRINZA , Abizar LAKDAWALLA , Steven MENCHEN , Gilad ALMOGY , Mark PRATT
CPC分类号： G01N33/492 , G01N1/02 , G01Q10/04 , G01N2001/2291
摘要： Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.
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公开(公告)号：US20220348994A1
公开(公告)日：2022-11-03
申请号：US17712765
申请日：2022-04-04
发明人： Linda LEE , Steven MENCHEN , Theo NIKIFOROV , Gilad ALMOGY , Florian OBERSTRASS
IPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6869 , G01N21/64
摘要： The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.
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