公开(公告)号：US11352601B2

公开(公告)日：2022-06-07

申请号：US16634027

申请日：2018-07-27

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding ,  Guang Yang

IPC分类号： C12N15/52 ,  C12N1/12 ,  C12N9/12 ,  C12R1/89

摘要： The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.

公开(公告)号：US20210163451A1

公开(公告)日：2021-06-03

申请号：US17049708

申请日：2019-05-16

申请人： University of Florida Research Foundation, Inc.

发明人： Yousong Ding ,  Guangde Jiang

IPC分类号： C07D403/06 ,  A01N43/60

摘要： Thaxtomins are phytotoxic secondary metabolites produced in plant pathogenic Streptomyces strains and have received considerable interests as bioherbicides. A cell-free, biocombinatorial approach was developed to produce a thaxtomin library for herbicide development. Combination of biosynthetic enzymes led to the production of 136 substituted 2,5-diketopiperazines, thaxtomin D, thaxtomin B and thaxtomin A analogs in a single pot. Furthermore, rational engineering of TxtA allowed the synthesis of azido-containing thaxtomin analog. Selected unnatural thaxtomins demonstrated improved herbicidal activities. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

公开(公告)号：US10550079B2

公开(公告)日：2020-02-04

申请号：US16174350

申请日：2018-10-30

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding

IPC分类号： C12N5/00 ,  C07D209/16 ,  C12N15/00 ,  C07D401/04 ,  C12N9/02 ,  A61K31/4045 ,  A61K31/405

摘要： The disclosure relates to the field of fusion proteins. In some aspects, the invention to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosures relates to compounds produced by artificial cytochrome P450 enzymes.

公开(公告)号：US20180044291A1

公开(公告)日：2018-02-15

申请号：US15552081

申请日：2016-02-18

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding

IPC分类号： C07D209/16 ,  A61K31/405 ,  A61K31/4045 ,  C07D401/04 ,  C12N9/02

CPC分类号： C07D209/16 ,  A61K31/4045 ,  A61K31/405 ,  C07D401/04 ,  C12N5/00 ,  C12N9/0004 ,  C12N15/00

摘要： The disclosure relates to the field of fusion proteins. In some aspects, the invention relates to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosure relates to compounds produced by artificial cytochrome P450 enzymes.

公开(公告)号：US20190292527A1

公开(公告)日：2019-09-26

申请号：US16345224

申请日：2017-10-26

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding ,  Ran Zuo

IPC分类号： C12N9/02 ,  C12P17/10 ,  C12P17/18 ,  C12P17/16

摘要： The disclosure relates to the field of fusion proteins. In some aspects, the disclosure relates to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosure relates to corn-pounds produced by artificial cytochrome P450 enzymes.

公开(公告)号：US20190119208A1

公开(公告)日：2019-04-25

申请号：US16174350

申请日：2018-10-30

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding

IPC分类号： C07D209/16 ,  A61K31/405 ,  A61K31/4045 ,  C12N5/00 ,  C12N9/02 ,  C12N15/00 ,  C07D401/04

CPC分类号： C07D209/16 ,  A61K31/4045 ,  A61K31/405 ,  C07D401/04 ,  C12N5/00 ,  C12N9/0004 ,  C12N15/00

摘要： The disclosure relates to the field of fusion proteins. In some aspects, the invention to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosures relates to compounds produced by artificial cytochrome P450 enzymes.

公开(公告)号：US11879144B2

公开(公告)日：2024-01-23

申请号：US18152329

申请日：2023-01-10

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding ,  Ran Zuo

IPC分类号： C12P13/22 ,  C12N9/04 ,  C12N9/02 ,  C12N15/62

CPC分类号： C12P13/227 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0075 ,  C12N15/62 ,  C12Y101/9901 ,  C12Y106/02004 ,  C12Y114/13039

摘要： The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.

公开(公告)号：US20230287466A1

公开(公告)日：2023-09-14

申请号：US18152329

申请日：2023-01-10

申请人： UNIVERSITY OF FLORIDA RESEARCH

发明人： Yousong Ding ,  Ran Zuo

IPC分类号： C12P13/22 ,  C12N9/04 ,  C12N9/02 ,  C12N15/62

CPC分类号： C12P13/227 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0075 ,  C12N15/62 ,  C12Y101/9901 ,  C12Y106/02004 ,  C12Y114/13039

摘要： The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.

公开(公告)号：US11655477B2

公开(公告)日：2023-05-23

申请号：US16616059

申请日：2018-05-23

申请人： University of Florida Research Foundation, Inc.

发明人： Rosemary Loria ,  Yucheng Zhang ,  Yousong Ding ,  Guangde Jiang

IPC分类号： C12N15/76 ,  C12N1/20 ,  C12P17/16 ,  A01N63/28

CPC分类号： C12N15/76 ,  A01N63/28 ,  C12N1/20 ,  C12P17/165 ,  C12N2510/02

摘要： The present disclosure includes genetically engineered, non-pathogenic Streptomyces bacterium with exogenous, non-native Thaxtomin A (ThxA) biosynthetic gene clusters conferring the genetically engineered, non-pathogenic Streptomyces bacterium with the ability to produce thaxtomin A. Also included are methods of providing thaxtomin producing capability in non-native Streptomyces bacterial strains, methods of producing thaxtomin compounds with the genetically engineered Streptomyces bacteria of the present disclosure, and methods of producing thaxtomin compounds and nitro-tryptophan analogs, and fluorinated thaxtomin compounds, analogs, and intermediates with the genetically engineered Streptomyces bacteria of the present disclosure.

公开(公告)号：US11512119B2

公开(公告)日：2022-11-29

申请号：US16633993

申请日：2018-07-26

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding ,  Peilan Zhang ,  Steven Douglas Bruner

IPC分类号： C07K14/37 ,  A61P35/00 ,  A61K38/16 ,  A61P35/02 ,  G01N33/574

摘要： The subject invention pertains to compositions and methods for preparing and using recombinant proteins based on the fungal Coprinus comatus Y3 protein to control plant and animal viruses and microbes, and diagnose, prevent and treat cancers. Methods are disclosed using compositions comprising recombinant Y3 proteins to diagnose, prevent and/or treat cancer diseases based on recombinant Y3 protein interaction with glycans expressed on cancer cells.