公开(公告)号：US20230407295A1

公开(公告)日：2023-12-21

申请号：US18141166

申请日：2023-04-28

Applicant: UNIVERSITY OF MASSACHUSETTS

Inventor： Han Zhang ,  Erik Joseph Sontheimer ,  Jonathan Kenneth Watts ,  Anastasia Khvorova

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/90

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12N15/907 ,  C12N2310/322 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/20

Abstract: Protecting oligonucleotides are provided. Protecting oligonucleotides with 5′ and/or 3′ conjugated moieties are provided. Protecting oligonucleotides with chemical modifications are provided. Methods of using the protecting oligonucleotides for genome editing with a CRISPR nuclease and kits for performing the same are also provided.

公开(公告)号：US20210388348A1

公开(公告)日：2021-12-16

申请号：US17318846

申请日：2021-05-12

Applicant: UNIVERSITY OF MASSACHUSETTS

Inventor： Erik Joseph Sontheimer ,  Anastasia Khvorova ,  Jonathan Kenneth Watts ,  Nadia Amrani ,  Zexiang Chen ,  Matthew Hassler ,  Dimas Echeverria Moreno ,  Julia Frances Alterman ,  Scot Wolfe ,  Ken Yamada ,  Gitali Devi ,  Han Zhang

IPC: C12N15/11 ,  C12N15/90 ,  C12N9/22

Abstract: Chemically modified crRNAs and tracrRNAs are provided. crRNAs and tracrRNAs with 5′ and/or 3′ conjugated moieties are provided. crRNAs and tracrRNAs with modifications in the repeat region of the crRNA or the anti-repeat region of the tracrRNA are provided. Methods of using the crRNAs and tracrRNAs for genome editing with a CRISPR nuclease and kits for performing the same are also provided.

公开(公告)号：US20160289734A1

公开(公告)日：2016-10-06

申请号：US15089243

申请日：2016-04-01

Applicant: University of Massachusetts

Inventor： Phillip David Zamore ,  Melissa Jeanne Moore ,  Samson Michael Jolly ,  William Edward Salomon ,  Victor Serebrov ,  Han Zhang

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/683 ,  C12N15/10 ,  C12N15/102 ,  C12N15/64 ,  C12Q1/6806 ,  C12Q1/6818 ,  G01N33/5308

Abstract: The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA depletion. Using such Argonaute polypeptide:guide molecule complexes enables fast and specific detection, purification, and enzymatic activity.

Abstract translation: 本发明涉及使用Argonaute多肽：指导分子复合物作为快速和特异性核酸探针，作为DNA和RNA底物的特异性核酸指导限制酶，以及作为检测RNA-蛋白质相互作用，RNA检测， DNA检测和RNA耗尽。 使用这样的Argonaute多肽：指导分子复合物可以快速，具体的检测，纯化和酶活性。