公开(公告)号：US20240361226A1

公开(公告)日：2024-10-31

申请号：US18765836

申请日：2024-07-08

Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Nathan SWAMI ,  Walter VARHUE ,  Vahid FARMEHINI

IPC: G01N15/1031 ,  B01L3/00 ,  G01N15/01 ,  G01N33/483 ,  G01R27/16

CPC classification number: G01N15/1031 ,  B01L3/502715 ,  B01L3/502761 ,  G01N33/4833 ,  G01R27/16 ,  B01L2200/0647 ,  B01L2300/0627 ,  G01N15/01

Abstract: An exemplary method and system is disclosed that facilitate the integration of on-chip impedance sensors and measurement circuitries, e.g., in characterizing internal microfluidic structures and/or in cell or particle cytometry, for quantifying the impedance/frequency response of microfluidic device under the same, or similar, conditions used for particle manipulation. In some embodiments, the exemplary method and system employs a circuit configured for automated determination and quantification of parasitic voltage drops during AC electrokinetic particle manipulation, without the need to use valuable biological samples or model particles. The determined impedance response can be used to assess efficacy of the microfluidic device geometry as well as to provide control signals to inform downstream cell separation decisions.

公开(公告)号：US20220034780A1

公开(公告)日：2022-02-03

申请号：US17280480

申请日：2019-09-26

Applicant: Nathan SWAMI ,  John MCGRATH ,  Walter VARHUE ,  Carlos HONRADO ,  Vahid FARMEHINI ,  Yi LIU ,  UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Nathan SWAMI ,  John MCGRATH ,  Walter VARHUE ,  Carlos HONRADO ,  Vahid FARMEHINI ,  Yi LIU

IPC: G01N15/10 ,  B01L3/00

Abstract: An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

公开(公告)号：US20240426735A1

公开(公告)日：2024-12-26

申请号：US18642197

申请日：2024-04-22

Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Nathan SWAMI ,  John MCGRATH ,  Walter VARHUE ,  Carlos HONRADO ,  Vahid FARMEHINI ,  Yi LIU

IPC: G01N15/10 ,  B01L3/00 ,  G01N15/1031

Abstract: An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

公开(公告)号：US20220091014A1

公开(公告)日：2022-03-24

申请号：US17425414

申请日：2020-01-24

Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Nathan SWAMI ,  Walter VARHUE ,  Vahid FARMEHINI

IPC: G01N15/10 ,  B01L3/00 ,  G01N33/483 ,  G01R27/16

Abstract: An exemplary method and system is disclosed that facilitate the integration of on-chip impedance sensors and measurement circuitries, e.g., in characterizing internal microfluidic structures and/or in cell or particle cytometry, for quantifying the impedance/frequency response of microfluidic device under the same, or similar, conditions used for particle manipulation. In some embodiments, the exemplary method and system employs a circuit configured for automated determination and quantification of parasitic voltage drops during AC electrokinetic particle manipulation, without the need to use valuable biological samples or model particles. The determined impedance response can be used to assess efficacy of the microfluidic device geometry as well as to provide control signals to inform downstream cell separation decisions.