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公开(公告)号：US20150125952A1
公开(公告)日：2015-05-07
申请号：US14390490
申请日：2013-03-15
发明人： Deok-Ho Kim , Michael Laflamme , Charles Murry , Kshitiz Gupta , Hyok Yoo , Alex Jiao
IPC分类号： C12N5/0735 , C12N5/074 , C12N5/077
CPC分类号： C12N5/0606 , A61K48/00 , A61L27/14 , A61L27/18 , A61L27/3826 , A61L27/3834 , A61L27/3873 , A61L27/50 , A61L2400/12 , A61L2400/18 , B01L3/5085 , B01L2300/0851 , B01L2300/0896 , B01L2400/086 , C12M21/08 , C12M25/14 , C12N5/0657 , C12N5/0658 , C12N5/0696 , C12N2503/02 , C12N2533/30 , C12N2533/40 , C12N2535/00 , C08L67/04
摘要： The present invention generally relates to the field of cell growth and tissue engineering, in particular, tissue engineered compositions comprising a nanotextured substrate which is structurally configured for growth of cells in an anatomically correct adult phenotype in vitro. In particular, described herein are nanotextured substrates which are structurally configured for the anisotropic organization, maturation, and growth of in vitro-differentiated muscle cells, such as cardiomyocytes, and methods for the production and use thereof in varying sizes, nanotextures and substrate rigidities. In vitro-differentiated cardiomyocytes grown on the nanotextured substrates described herein are better-differentiated and more closely mimic adult cardiac tissue than the same cells grown on a non-textured substrate of the same composition. The nanotextured substrate/cell constructs provide a platform for screening to predict the effect of test agents or drugs on, for example, human cardiac tissue, including patient-derived tissue, or for the identification of agents that effect various cardiac functional parameters.
摘要翻译： 本发明一般涉及细胞生长和组织工程领域，特别是包括纳米纹理底物的组织工程化组合物，其在结构上被配置用于体外在解剖学正确的成体表型中生长细胞。 特别地，本文描述的是纳米纹理的底物，其被结构地配置用于体外分化的肌细胞（例如心肌细胞）的各向异性组织，成熟和生长，以及用于以不同大小，纳米纹理和底物刚性生产和使用它们的方法。 在本文所述的纳米纹理底物上生长的体外分化的心肌细胞比在相同组成的非纹理底物上生长的相同细胞更好地分化并且更接近地模拟成体心脏组织。 纳米纹理底物/细胞构建体提供筛选的平台以预测测试剂或药物对例如人心脏组织（包括患者衍生的组织）或用于鉴定影响各种心脏功能参数的试剂的影响。
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公开(公告)号：US09994812B2
公开(公告)日：2018-06-12
申请号：US14390490
申请日：2013-03-15
发明人： Deok-Ho Kim , Michael Laflamme , Charles Murry , Kshitiz Gupta , Hyok Yoo , Alex Jiao
IPC分类号： A61K48/00 , C12N5/0735 , A61L27/14 , C12M3/00 , C12M1/12 , A61L27/18 , A61L27/38 , A61L27/50 , B01L3/00 , C12N5/077 , C12N5/074
CPC分类号： C12N5/0606 , A61K48/00 , A61L27/14 , A61L27/18 , A61L27/3826 , A61L27/3834 , A61L27/3873 , A61L27/50 , A61L2400/12 , A61L2400/18 , B01L3/5085 , B01L2300/0851 , B01L2300/0896 , B01L2400/086 , C12M21/08 , C12M25/14 , C12N5/0657 , C12N5/0658 , C12N5/0696 , C12N2503/02 , C12N2533/30 , C12N2533/40 , C12N2535/00 , C08L67/04
摘要： The present invention generally relates to the field of cell growth and tissue engineering, in particular, tissue engineered compositions comprising a nanotextured substrate which is structurally configured for growth of cells in an anatomically correct adult phenotype in vitro. In particular, described herein are nanotextured substrates which are structurally configured for the anisotropic organization, maturation, and growth of in vitro-differentiated muscle cells, such as cardiomyocytes, and methods for the production and use thereof in varying sizes, nanotextures and substrate rigidities. In vitro-differentiated cardiomyocytes grown on the nanotextured substrates described herein are better-differentiated and more closely mimic adult cardiac tissue than the same cells grown on a non-textured substrate of the same composition. The nanotextured substrate/cell constructs provide a platform for screening to predict the effect of test agents or drugs on, for example, human cardiac tissue, including patient-derived tissue, or for the identification of agents that effect various cardiac functional parameters.
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