公开(公告)号：US20240398869A1

公开(公告)日：2024-12-05

申请号：US18526459

申请日：2023-12-01

Applicant: University Health Network

Inventor： Gordon Keller ,  Stephanie Protze ,  Jee Hoon Lee

IPC: A61K35/28 ,  A61K35/34 ,  A61P9/04 ,  C12N5/077 ,  G01N33/50 ,  G01N33/68

Abstract: Methods are disclosed for producing populations of cardiomyocytes from pluripotent stem cells. Populations may be enriched for either atrial or ventricular cardiomyocytes and the resulting ventricular population may be essentially free of pacemaker cells. The method includes incubating pluripotent stem cells in a suitable medium with a BMP component, and an activin component, the amounts of activin may be varied to enrich for either atrial or ventricular cardiomyocytes. The enriched populations, as well as methods of using the same to treat patients in need of cardiac repair are disclosed.

公开(公告)号：US11730770B2

公开(公告)日：2023-08-22

申请号：US16737768

申请日：2020-01-08

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： Gordon Keller ,  Stephanie Protze

IPC: A61K35/34 ,  C12N5/077 ,  C12N5/10

CPC classification number: A61K35/34 ,  C12N5/0657 ,  C12N5/10 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/385 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45

Abstract: Provided are methods for producing compositions comprising a population of cardiomyocytes enriched for or substantially devoid of sinoatrial node-like pacemaker cardiomyocytes (SANLCM) from human pluripotent stem cells (hPSCs), and methods of use thereof.

公开(公告)号：US10561687B2

公开(公告)日：2020-02-18

申请号：US15550572

申请日：2016-02-17

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： Gordon Keller ,  Stephanie Protze

IPC: C12N5/00 ,  A61K35/34 ,  C12N5/077 ,  C12N5/10

Abstract: Provided are methods for producing compositions comprising a population of cardiomyocytes enriched for or substantially devoid of sinoatrial node-like pacemaker cardiomyocytes (SANLCM) from human pluripotent stem cells (hPSCs), and methods of use thereof.

公开(公告)号：US20190336537A1

公开(公告)日：2019-11-07

申请号：US16466278

申请日：2017-12-04

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： Gordon Keller ,  Stephanie Protze ,  Jee Hoon Lee

IPC: A61K35/28 ,  A61K35/34 ,  G01N33/50 ,  G01N33/68 ,  A61P9/04 ,  C12N5/077

Abstract: Methods are disclosed for producing populations of cardiomyocytes from pluripotent stem cells. Populations may be enriched for either atrial or ventricular cardiomyocytes and the resulting ventricular population may be essentially free of pacemaker cells. The method includes incubating pluripotent stem cells in a suitable medium with a BMP component, and an activin component, the amounts of activin may be varied to enrich for either atrial or ventricular cardiomyocytes. The enriched populations, as well as methods of using the same to treat patients in need of cardiac repair are disclosed.