公开(公告)号：US20230024611A1

公开(公告)日：2023-01-26

申请号：US17791079

申请日：2021-03-02

Applicant: University of Kansas

Inventor： Yong Zeng

IPC: G01N33/574 ,  G01N33/68 ,  G01N33/543 ,  C12Q1/37 ,  G01N21/64

Abstract: Provided herein are methods for inkjet printing objects, including objects which may be used as elements of microfluidic devices. The microfluidic devices incorporating the elements are also provided. Such microfluidic devices include those configured to quantify the expression and activity of exosomal matrix metalloprotease, MMP14. These microfluidic devices may be used in methods of monitoring breast cancer in patients having breast cancer.

公开(公告)号：US10350599B2

公开(公告)日：2019-07-16

申请号：US15357231

申请日：2016-11-21

Applicant: University of Kansas ,  Kansas State University Research Foundation

Inventor： Zheng Zhao ,  Mei He ,  Yong Zeng

IPC: B01F5/06 ,  G01N33/574 ,  B01L3/00 ,  B01F13/00

Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyzes of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.

公开(公告)号：US11726086B2

公开(公告)日：2023-08-15

申请号：US17032037

申请日：2020-09-25

Applicant: The University of Kansas ,  Kansas State University Research Foundation

Inventor： Peng Zhang ,  Yong Zeng ,  Mei He

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/68

CPC classification number: G01N33/54393 ,  B01L3/502707 ,  B01L3/502761 ,  G01N33/54326 ,  G01N33/54353 ,  G01N33/6872 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887

Abstract: A graphene-based sandwich immunoassay for detecting whether a target biological substance is present in a sample, generally comprising contacting said sample with a plurality of particles coated with graphene nanosheets, each particle having at least one targeting receptor, such that the target biological substance, if present, associates with the targeting receptor, and detecting the presence of the target biological substance in the sample by subsequently contacting the sample with a detection antibody, wherein the detection antibody is capable of targeting and binding with the target biological substance if bound to the targeting receptor to yield a detectable complex. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome.

公开(公告)号：US20170065978A1

公开(公告)日：2017-03-09

申请号：US15357231

申请日：2016-11-21

Applicant: University of Kansas ,  Kansas State University Research Foundation

Inventor： Zheng Zhao ,  Mei He ,  Yong Zeng

IPC: B01L3/00 ,  G01N33/574

CPC classification number: B01L3/502761 ,  B01F5/0647 ,  B01F13/0059 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/043 ,  B01L2400/0487 ,  G01N33/574

Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.

Abstract translation: 公开了一种结合外来体分离和靶向蛋白质组学分析的微流体外显子分析平台。 该平台能够直接从血浆样品中进行定量的外来生物标志物分析，具有显着增强的敏感性和特异性。 通过在所选择的亚群中探查表面蛋白和胞内生物标志物的多参数分析来证明患者来源的外来体的不同亚群的鉴定。 在非小细胞肺癌（NSCLC）患者血浆中IGF-1R的表达及其磷酸化水平被评估为常规活检和免疫组织化学的非侵入性替代物。 卵巢癌的检测也进行了评估。 可以由玻璃基板和聚（二甲基硅氧烷）层制成的微流体芯片包括用于混合流体的蛇形微通道和用于收集和检测外来体的微型室。

公开(公告)号：US10788486B2

公开(公告)日：2020-09-29

申请号：US15728155

申请日：2017-10-09

Applicant: The University of Kansas ,  Kansas State University Research Foundation

Inventor： Peng Zhang ,  Yong Zeng ,  Mei He

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/68

Abstract: A capture device for capturing a biological substance can include: a substrate; a graphene-oxide layer on the substrate; at least one polydopamine polymer coupled with the graphene-oxide; and at least one targeting receptor coupled to the polydopamine(s), wherein the targeting receptor is capable of targeting/binding with a target biological substance. The graphene-oxide may be covalently coupled with the substrate and polydopamine, and the polydopamine may be covalently coupled with the targeting receptor. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome. The substrate can be a particle (e.g., magnetic, such as magnetically responsive) or a surface in a microfluidic channel. The surface can be a top surface of a post, the post having a Y-shaped cross-sectional profile. In one aspect, the substrate is a particle. The capture device can include the target biological substance bound to the targeting receptor.

公开(公告)号：US10434511B2

公开(公告)日：2019-10-08

申请号：US15125822

申请日：2015-03-16

Applicant: University of Kansas

Inventor： Mei He ,  Yong Zeng ,  Andrew Godwin

IPC: B01L3/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/574

Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from 30 μL plasma samples within approximately 100 minutes with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed, as a non-invasive alternative to the conventional biopsy and immunohistochemistry. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), can include a first capture chamber, a second capture chamber, a serpentine microchannel, a first microchannel, a second microchannel, a sample inlet, a buffer inlet, a bead inlet, at least a first connector channel, and a reagent inlet.

公开(公告)号：US20180100853A1

公开(公告)日：2018-04-12

申请号：US15728155

申请日：2017-10-09

Applicant: The University of Kansas ,  Kansas State University Research Foundation

Inventor： Peng Zhang ,  Yong Zeng ,  Mei He

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/68

CPC classification number: G01N33/54393 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  G01N33/54326 ,  G01N33/54353 ,  G01N33/6872

Abstract: A capture device for capturing a biological substance can include: a substrate; a graphene-oxide layer on the substrate; at least one polydopamine polymer coupled with the graphene-oxide; and at least one targeting receptor coupled to the polydopamine(s), wherein the targeting receptor is capable of targeting/binding with a target biological substance. The graphene-oxide may be covalently coupled with the substrate and polydopamine, and the polydopamine may be covalently coupled with the targeting receptor. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome. The substrate can be a particle (e.g., magnetic, such as magnetically responsive) or a surface in a microfluidic channel. The surface can be a top surface of a post, the post having a Y-shaped cross-sectional profile. In one aspect, the substrate is a particle. The capture device can include the target biological substance bound to the targeting receptor.

公开(公告)号：US20210018501A1

公开(公告)日：2021-01-21

申请号：US17032037

申请日：2020-09-25

Applicant: The University of Kansas ,  Kansas State University Research Foundation

Inventor： Peng Zhang ,  Yong Zeng ,  Mei He

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/68

Abstract: A graphene-based sandwich immunoassay for detecting whether a target biological substance is present in a sample, generally comprising contacting said sample with a plurality of particles coated with graphene nanosheets, each particle having at least one targeting receptor, such that the target biological substance, if present, associates with the targeting receptor, and detecting the presence of the target biological substance in the sample by subsequently contacting the sample with a detection antibody, wherein the detection antibody is capable of targeting and binding with the target biological substance if bound to the targeting receptor to yield a detectable complex. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome.