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    Graphene oxide-based nanolab and methods of detecting of exosomes
    2.
    发明授权
    Graphene oxide-based nanolab and methods of detecting of exosomes 有权

    公开(公告)号：US10788486B2

    公开(公告)日：2020-09-29

    申请号：US15728155

    申请日：2017-10-09

    Applicant: The University of Kansas ,  Kansas State University Research Foundation

    Inventor： Peng Zhang ,  Yong Zeng ,  Mei He

    IPC: G01N33/543 ,  B01L3/00 ,  G01N33/68

    Abstract: A capture device for capturing a biological substance can include: a substrate; a graphene-oxide layer on the substrate; at least one polydopamine polymer coupled with the graphene-oxide; and at least one targeting receptor coupled to the polydopamine(s), wherein the targeting receptor is capable of targeting/binding with a target biological substance. The graphene-oxide may be covalently coupled with the substrate and polydopamine, and the polydopamine may be covalently coupled with the targeting receptor. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome. The substrate can be a particle (e.g., magnetic, such as magnetically responsive) or a surface in a microfluidic channel. The surface can be a top surface of a post, the post having a Y-shaped cross-sectional profile. In one aspect, the substrate is a particle. The capture device can include the target biological substance bound to the targeting receptor.

    Non-invasive monitoring cancer using integrated microfluidic profiling of circulating microvesicles
    3.
    发明授权
    Non-invasive monitoring cancer using integrated microfluidic profiling of circulating microvesicles 有权

    公开(公告)号：US10434511B2

    公开(公告)日：2019-10-08

    申请号：US15125822

    申请日：2015-03-16

    Applicant: University of Kansas

    Inventor： Mei He ,  Yong Zeng ,  Andrew Godwin

    IPC: B01L3/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/574

    Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from 30 μL plasma samples within approximately 100 minutes with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed, as a non-invasive alternative to the conventional biopsy and immunohistochemistry. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), can include a first capture chamber, a second capture chamber, a serpentine microchannel, a first microchannel, a second microchannel, a sample inlet, a buffer inlet, a bead inlet, at least a first connector channel, and a reagent inlet.

    METHODS FOR GENERATING THERAPEUTIC DELIVERY PLATFORMS
    5.
    发明申请
    METHODS FOR GENERATING THERAPEUTIC DELIVERY PLATFORMS 有权

    公开(公告)号：US20210268121A1

    公开(公告)日：2021-09-02

    申请号：US17236561

    申请日：2021-04-21

    Applicant: The University of Kansas ,  Kansas State University Research Foundation

    Inventor： Mei He

    IPC: A61K47/69 ,  G01N33/543 ,  G01N33/574

    Abstract: Methods for producing engineered exosomes and other vesicle-like biological targets, including allowing a target vesicle-like structure to react and bind with immunomagnetic particles; capturing the immunomagnetic particle/vesicle complex by applying a magnetic field; further engineering the captured vesicles by surface modifying with additional active moieties or internally loading with active agents; and releasing the engineered vesicle-like structures, such as by photolytically cleaving a linkage between the particle and engineered vesicle-like structures, thereby releasing intact vesicle-like structures which can act as delivery vehicles for therapeutic treatments.

    GRAPHENE OXIDE-BASED NANOLAB AND METHODS OF DETECTING OF EXOSOMES
    6.
    发明申请
    GRAPHENE OXIDE-BASED NANOLAB AND METHODS OF DETECTING OF EXOSOMES 有权

    公开(公告)号：US20210018501A1

    公开(公告)日：2021-01-21

    申请号：US17032037

    申请日：2020-09-25

    Applicant: The University of Kansas ,  Kansas State University Research Foundation

    Inventor： Peng Zhang ,  Yong Zeng ,  Mei He

    IPC: G01N33/543 ,  B01L3/00 ,  G01N33/68

    Abstract: A graphene-based sandwich immunoassay for detecting whether a target biological substance is present in a sample, generally comprising contacting said sample with a plurality of particles coated with graphene nanosheets, each particle having at least one targeting receptor, such that the target biological substance, if present, associates with the targeting receptor, and detecting the presence of the target biological substance in the sample by subsequently contacting the sample with a detection antibody, wherein the detection antibody is capable of targeting and binding with the target biological substance if bound to the targeting receptor to yield a detectable complex. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome.

    NON-INVASIVE MONITORING CANCER USING INTEGRATED MICROFLUIDIC PROFILING OF CIRCULATING MICROVESICLES
    8.
    发明申请
    NON-INVASIVE MONITORING CANCER USING INTEGRATED MICROFLUIDIC PROFILING OF CIRCULATING MICROVESICLES 审中-公开
    使用综合微循环微循环的非侵入式监测癌症

    公开(公告)号：US20170065978A1

    公开(公告)日：2017-03-09

    申请号：US15357231

    申请日：2016-11-21

    Applicant: University of Kansas ,  Kansas State University Research Foundation

    Inventor： Zheng Zhao ,  Mei He ,  Yong Zeng

    IPC: B01L3/00 ,  G01N33/574

    CPC classification number: B01L3/502761 ,  B01F5/0647 ,  B01F13/0059 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/043 ,  B01L2400/0487 ,  G01N33/574

    Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.

    Abstract translation: 公开了一种结合外来体分离和靶向蛋白质组学分析的微流体外显子分析平台。 该平台能够直接从血浆样品中进行定量的外来生物标志物分析，具有显着增强的敏感性和特异性。 通过在所选择的亚群中探查表面蛋白和胞内生物标志物的多参数分析来证明患者来源的外来体的不同亚群的鉴定。 在非小细胞肺癌（NSCLC）患者血浆中IGF-1R的表达及其磷酸化水平被评估为常规活检和免疫组织化学的非侵入性替代物。 卵巢癌的检测也进行了评估。 可以由玻璃基板和聚（二甲基硅氧烷）层制成的微流体芯片包括用于混合流体的蛇形微通道和用于收集和检测外来体的微型室。

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