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    Methods for isolating pancreatic islets
    1.
    发明授权
    Methods for isolating pancreatic islets 有权

    公开(公告)号：US11590501B2

    公开(公告)日：2023-02-28

    申请号：US17445972

    申请日：2021-08-26

    申请人： University of Virginia Patent Foundation

    发明人： Nathan Swami ,  Walter Varhue ,  Linda W. Langman ,  Kenneth Brayman ,  Shayn Peirce-Cottler

    IPC分类号： B01L3/00 ,  G01N15/02 ,  G01N15/00

    摘要： A microfluidic device can include an upstream passage, a sample passage, a bifurcating passage, and a combining passage. The upstream passage can be configured to provide a focusing stream. The sample passage can be configured to provide a sample stream. The bifurcating passage can include a specified bifurcating flow resistance. The combining passage can be configured to create a combined stream from the focusing stream and the sample stream, where the focusing stream can direct the sample stream away from the upstream passage and toward the bifurcating passage. A first portion of the combined stream can be discharged through the bifurcating passage. The main discharge can be configured to discharge a second portion of the combined stream. The main discharge can include a main discharge resistance that is selectable to vary the main discharge resistance relative to the bifurcating flow resistance.

    COMPOSITIONS AND METHODS FOR PROMOTING ISLET VIABILITY AND ENHANCING INSULIN SECRETION
    3.
    发明申请
    COMPOSITIONS AND METHODS FOR PROMOTING ISLET VIABILITY AND ENHANCING INSULIN SECRETION 有权

    公开(公告)号：US20220160838A1

    公开(公告)日：2022-05-26

    申请号：US17438760

    申请日：2020-03-13

    申请人： University of Virginia Patent Foundation

    发明人： Gordon W. Laurie ,  Kenneth Brayman ,  Preeti Chhabra ,  Mingyang Ma ,  Karina Teixeira ,  Thomas R. Gadek

    IPC分类号： A61K38/26 ,  A61K35/39

    摘要： Compositions and methods for regenerating pancreatic islet viability and/or cell proliferation in vitro, ex vivo, and/or in vivo; and/or for regenerating glucose-stimulated insulin secretion; and/or for regenerating viability and/or cell proliferation of a transplanted pancreatic islets; and/or for preventing and/or inhibiting rejection of a transplanted islets; and/or for pancreatic islet transplantation; and/or for treating a symptom of a condition, disorder, or disease associated with abnormal insulin responsiveness to glucose are provided. In some embodiments, the compositions include a peptide and/or a pharmaceutically acceptable salt thereof, and/or a biologically active fragment, analog, or derivative thereof, wherein the peptide, the pharmaceutically acceptable salt thereof, and/or the biologically active fragment, analog, or derivative thereof has an amino acid sequence of any of SEQ ID NOs: 1-60, or any combination thereof.

    COMPOSITIONS AND METHODS FOR MAKING AND USING OXYGEN SENSING NANOFIBERS AND SCAFFOLDS
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR MAKING AND USING OXYGEN SENSING NANOFIBERS AND SCAFFOLDS 有权
    制备和使用氧气感应纳米纤维和组分的组合物和方法

    公开(公告)号：US20160041177A1

    公开(公告)日：2016-02-11

    申请号：US14818980

    申请日：2015-08-05

    申请人： University of Virginia Patent Foundation

    发明人： Kenneth Brayman ,  Daniel Bowers ,  Cassandra L. Fraser ,  Edward A. Botchwey, III

    IPC分类号： G01N33/58 ,  A61K49/00

    CPC分类号： G01N33/582 ,  A61K49/0015 ,  A61K49/0021 ,  A61K49/0054 ,  A61K49/0095 ,  G01N33/583 ,  G01N33/84 ,  G01N2800/7038

    摘要： To address the need for scaffold-based oxygen concentration monitoring, a single-component, self-referenced oxygen sensor was made into nanofibers. Electrospinning process parameters were tuned to produce a biomaterial scaffold with specific morphological features. The ratio of an oxygen sensitive phosphorescence signal to an oxygen insensitive fluorescence signal was calculated at each image pixel to determine an oxygenation value. A single component boron dye-polymer conjugate was chosen for additional investigation due to improved resistance to degradation in aqueous media compared to a dye polymer blend. Standardization curves show that in fully supplemented media, the fibers are responsive to dissolved oxygen concentrations less than 15 parts per million. Spatial and temporal ratiometric gradients were observed in vitro radiating outward from the center of a dense adherent cell grouping. Sensor activation in ischemia and cell transplant models in vivo show oxygenation decreases on the scale of minutes.

    摘要翻译： 为了满足基于脚手架氧浓度监测的需要，将单组分自参考氧传感器制成纳米纤维。 调整电纺工艺参数以产生具有特定形态特征的生物材料支架。 在每个图像像素处计算氧敏感磷光信号与不敏感荧光信号的比率，以确定氧合值。 选择单组分硼染料 - 聚合物共轭物进行额外的研究，因为与染料聚合物共混物相比，水性介质中的降解性改善。 标准化曲线显示，在完全补充的培养基中，纤维对低于15ppm的溶解氧浓度有反应。 体外观察到空间和时间比例梯度，从密集贴壁细胞组的中心向外辐射。 局部缺血和细胞移植模型中的传感器活化在体内显示出氧合作用以分钟的规模降低。