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公开(公告)号：US12031896B2
公开(公告)日：2024-07-09
申请号：US17425414
申请日：2020-01-24
Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION
Inventor： Nathan Swami , Walter Varhue , Vahid Farmehini
IPC: B01L3/00 , G01N15/01 , G01N15/1031 , G01N33/483 , G01R27/16
CPC classification number: G01N15/1031 , B01L3/502715 , B01L3/502761 , G01N33/4833 , G01R27/16 , B01L2200/0647 , B01L2300/0627 , G01N15/01
Abstract: Disclosed herein are methods and systems that facilitate the integration of on-chip impedance sensors and measurement circuitries for quantifying the impedance/frequency response of microfluidic device under the same, or similar, conditions used for particle manipulation. The methods and systems can use a microfluidic chip comprising a microfluidic channel with one or more electric-field-generating structures located therein, including a first electric-field-generating structure, wherein the one or more electric-field-generating structures is configured to selectively polarize or manipulate biologic or particle components flowing within the microfluidic channel. The method and system can also employ a circuit configured for automated determination and quantification of parasitic voltage drops during AC electrokinetic particle manipulation, without the need to use valuable biological samples or model particles. The determined impedance response can be used to assess efficacy of the microfluidic device geometry as well as to provide control signals to inform downstream cell separation decisions.
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公开(公告)号：US20240375104A1
公开(公告)日：2024-11-14
申请号：US18691779
申请日：2022-09-13
Applicant: Nathan SWAMI , James P. LANDERS , Vahid FARMEHINI , Sadie Marie KIENDZIOR , UNIVERSITY OF VIRGINIA , University Of Virginia Patent Foundation
Inventor： Nathan Swami , James P. Landers , Vahid Farmehini , Sadie Marie Kiendzior
Abstract: A circuit implementation for on-chip real-time resonance frequency measurement and feedback control may be used to provide improved selective particle trapping. The circuit may be based on monitoring current drawn from the amplifier used to stimulate the piezo transducer, as the high impedance measurement sensitivity in this mode does not lower the power available for stimulation of the piezo transducer. The enhanced level of control of acoustic trapping using this current mode may be used for various localized channel perturbations, including drift, wash steps and buffer swaps, as well as for selective sperm cell trapping from a heterogeneous sample that includes lysed epithelial cells.
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公开(公告)号：US11590501B2
公开(公告)日：2023-02-28
申请号：US17445972
申请日：2021-08-26
Applicant: University of Virginia Patent Foundation
Inventor： Nathan Swami , Walter Varhue , Linda W. Langman , Kenneth Brayman , Shayn Peirce-Cottler
Abstract: A microfluidic device can include an upstream passage, a sample passage, a bifurcating passage, and a combining passage. The upstream passage can be configured to provide a focusing stream. The sample passage can be configured to provide a sample stream. The bifurcating passage can include a specified bifurcating flow resistance. The combining passage can be configured to create a combined stream from the focusing stream and the sample stream, where the focusing stream can direct the sample stream away from the upstream passage and toward the bifurcating passage. A first portion of the combined stream can be discharged through the bifurcating passage. The main discharge can be configured to discharge a second portion of the combined stream. The main discharge can include a main discharge resistance that is selectable to vary the main discharge resistance relative to the bifurcating flow resistance.
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公开(公告)号：US20230287473A1
公开(公告)日：2023-09-14
申请号：US18006236
申请日：2021-07-21
Applicant: University of Virginia Patent Foundation
Inventor： Nathan Swami , Cirle Alcantrara Warren , John Hudson Moore, II , Carlos Honrado , Armita Salahi
CPC classification number: C12Q1/04 , G01N15/1031 , G01N2015/1006
Abstract: A method for quantifying bacterial spore germination can include creating an ex vivo assay including bacteria spores and a homogenized stool sample. The ex vivo assay can be loaded into a microfluidic chip. Vegetative bacteria and the bacteria spores can be detected by sampling the mixture in the microfluidic chip using impedance cytometry to assess disruption of host microbiota.
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公开(公告)号：US11339417B2
公开(公告)日：2022-05-24
申请号：US15515528
申请日：2015-10-09
Applicant: University of Virginia Patent Foundation
Inventor： Nathan Swami , Yi-Hsuan Su , Cirle Alcantara Warren , Ali Rohani , Vahid Farmehini
IPC: B03C5/00 , B03C5/02 , C12Q1/04 , G01N33/569 , G01N33/487
Abstract: Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.
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Patent Agency Ranking
公开(公告)号：US11965810B2
公开(公告)日：2024-04-23
申请号：US17280480
申请日：2019-09-26
Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION , Nathan Swami , John McGrath , Walter Varhue , Carlos Honrado , Vahid Farmehini , Yi Liu
Inventor： Nathan Swami , John McGrath , Walter Varhue , Carlos Honrado , Vahid Farmehini , Yi Liu
IPC: G01N15/00 , B01L3/00 , G01N15/10 , G01N15/1031
CPC classification number: G01N15/1056 , B01L3/502761 , G01N15/1031 , B01L2200/027 , B01L2200/0652 , B01L2200/10 , B01L2300/0645 , B01L2400/0424
Abstract: An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.
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公开(公告)号：US11759566B2
公开(公告)日：2023-09-19
申请号：US17050082
申请日：2019-05-29
Applicant: University of Virginia Patent Foundation
Inventor： Edward H. Bertram , Nathan Swami , Walter Varhue
CPC classification number: A61M5/16813 , A61M5/142 , A61M25/02 , A61M2025/024 , A61M2205/3334 , A61M2205/52 , A61M2210/0693
Abstract: System and method to improve drug delivery to identified regions in the brain or elsewhere through direct infusion of a therapeutic agent or the like into that region. This direct infusion will allow for greater concentrations of the agent in the target region while reducing concentrations elsewhere in the body where these agents may be toxic. The system and method improves efficacy while reducing unwanted side effects. The system includes an array of multiple, independently targeted, microporous catheters for insertion into the target region and a distribution system that allows for individualized flow control to each catheter. The system may be connected to a reservoir that contains the therapeutic agent, and flow to the system is maintained through one or more pumps. This system will greatly improve on the current single catheter infusion design and shall provide therapy, delivered through multiple catheters, thus delivering the therapy evenly over a customizable volume.
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公开(公告)号：US20210093778A1
公开(公告)日：2021-04-01
申请号：US17050082
申请日：2019-05-29
Applicant: University of Virginia Patent Foundation
Inventor： Edward H. Bertram , Nathan Swami , Walter Varhue
Abstract: System and method to improve drug delivery to identified regions in the brain or elsewhere through direct infusion of a therapeutic agent or the like into that region. This direct infusion will allow for greater concentrations of the agent in the target region while reducing concentrations elsewhere in the body where these agents may be toxic. The system and method improves efficacy while reducing unwanted side effects. The system includes an array of multiple, independently targeted, microporous catheters for insertion into the target region and a distribution system that allows for individualized flow control to each catheter. The system may be connected to a reservoir that contains the therapeutic agent, and flow to the system is maintained through one or more pumps. This system will greatly improve on the current single catheter infusion design and shall provide therapy, delivered through multiple catheters, thus delivering the therapy evenly over a customizable volume.
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9.发明申请公开(公告)号：US20170218424A1
公开(公告)日：2017-08-03
申请号：US15515528
申请日：2015-10-09
Applicant: University of Virginia Patent Foundation
Inventor： Nathan Swami , Yi-Hsuan Su , Cirle Alcantara Warren , Ali Rohani , Vahid Farmehini
CPC classification number: C12Q1/04 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N33/487 , G01N33/56911 , G01N2333/33
Abstract: Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.
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公开(公告)号：US12297481B2
公开(公告)日：2025-05-13
申请号：US17750880
申请日：2022-05-23
Applicant: University of Virginia Patent Foundation
Inventor： Nathan Swami , Ali Rohani , Vahid Farmehini , Walter Varhue
IPC: B03C5/00 , B03C5/02 , C12Q1/04 , G01N33/569 , G01N33/487
Abstract: Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.
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