公开(公告)号：US12031152B2

公开(公告)日：2024-07-09

申请号：US17341220

申请日：2021-06-07

申请人： Upside Foods, Inc.

发明人： Kalle Lukyan Johnson ,  Patricia R. Benton ,  Cameron Gentry Copeland ,  Sukhdeep Singh Dhadwar ,  Meri Teresa Firpo ,  Marie Elizabeth Gibbons ,  Stephen K. Hsu ,  Robert Sierra ,  Kevin John Kayser

IPC分类号： C12N5/071

CPC分类号： C12N5/0602 ,  C12N2500/14 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33

摘要： Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.

公开(公告)号：US20230203446A1

公开(公告)日：2023-06-29

申请号：US18146541

申请日：2022-12-27

申请人： Upside Foods, Inc.

发明人： Konrad Müller-Auffermann ,  Stephen K. Hsu ,  Kalle Lukyan Johnson ,  Matthew Leung ,  Jayanthi Lakkyreddy ,  Morgan Laurence Rease ,  Jessica Joslin

IPC分类号： C12N5/077 ,  C12N5/00 ,  A23L13/00

CPC分类号： C12N5/0652 ,  A23L13/00 ,  C12N5/0018 ,  C12N2500/00 ,  C12N2527/00

摘要： This disclosure relates to methods of washing cells from a grown cell mass to remove cell culture media and enriching the cells with a finishing media. The disclosed method includes growing a cell mass in cell culture media and then collecting and washing the grown cell mass with a series of wash buffers or a gradient wash buffer that changes over time. While the cell culture media contains nutrients and components beneficial for cell growth, cells grown in cell culture media often have off flavors, off aromas, poor color, poor salt/minerality compositions, and other shortcomings. Accordingly, the disclosed method comprises removing cell culture media remnants from a grown cell mass using a single or a series of wash media. The grown cell mass is further washed with a finishing or enrichment buffer to further improve sensory aspects and nutritional composition of the grown cell mass.