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    GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    5.
    发明申请
    GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 有权

    公开(公告)号：US20090311745A1

    公开(公告)日：2009-12-17

    申请号：US12300275

    申请日：2007-05-03

    申请人： Klaus Liebeton ,  Jürgen Eck ,  Uwe Bornscheuer ,  Dominique Böttcher ,  Peter Langer ,  Esen Bellur

    发明人： Klaus Liebeton ,  Jürgen Eck ,  Uwe Bornscheuer ,  Dominique Böttcher ,  Peter Langer ,  Esen Bellur

    IPC分类号： C12P21/00 ,  C12P7/62 ,  C12P7/40 ,  C12P7/02 ,  C12N15/00 ,  C12N15/74 ,  C12N9/18 ,  C12N1/21 ,  C12N15/63 ,  C07K16/40 ,  C07H21/00

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.

    Process for preparation to stereoisomeric carboxylic acid esters
    9.
    发明授权
    Process for preparation to stereoisomeric carboxylic acid esters 失效
    立体异构羧酸酯的制备方法

    公开(公告)号：US06365398B1

    公开(公告)日：2002-04-02

    申请号：US09505709

    申请日：2000-02-17

    申请人： Uwe Bornscheuer ,  Erik Henke ,  Hong Yang

    发明人： Uwe Bornscheuer ,  Erik Henke ,  Hong Yang

    IPC分类号： C12P4100

    CPC分类号： C12P7/62 ,  C12P41/005

    摘要： The present invention relates to a process for the preparation of stereoisomeric carboxylic acid esters consisting of an acid and of an alcohol component each having at least one chiral center, at least one stereoisomer of the carboxylic acid ester being present in an excess, by reacting a racemic carboxylic acid ester with a racemic alcohol in the presence of a carboxyl ester hydrolase (EC 3.1.1).

    摘要翻译： 本发明涉及制备由酸和醇组分组成的立体异构羧酸酯的方法，每一种具有至少一个手性中心，至少一种羧酸酯的立体异构体以过量存在，通过使 外消旋羧酸酯与外消旋醇在羧基酯水解酶（EC 3.1.1）存在下反应。