公开(公告)号：US11242511B2

公开(公告)日：2022-02-08

申请号：US16284999

申请日：2019-02-25

申请人： VIB VZW ,  UNIVERSITEIT GENT

发明人： Jan Tavernier ,  Samuel Lievens

IPC分类号： C12N9/12 ,  C12N15/10 ,  C12N15/62 ,  G01N33/542 ,  C12Q1/48

摘要： The disclosure relates to a cytoplasmic protein complex comprising: (a) a first recombinant fusion protein comprising a kinase, fused to a first interaction polypeptide; and (b) a second recombinant fusion protein comprising a domain comprising a reporter phosphorylation site, whereby the domain is fused to a second interaction polypeptide. The disclosure relates further to a method to detect compound-compound-interaction using the cytoplasmic protein complex, and to cells comprising such cytoplasmic protein complex.

公开(公告)号：US10336811B2

公开(公告)日：2019-07-02

申请号：US14381502

申请日：2013-03-06

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Samuel Lievens

IPC分类号： C12N9/02 ,  C07K14/72 ,  C12N15/62 ,  C07K14/705 ,  C12N9/12 ,  C12Q1/66 ,  G01N33/58 ,  G01N33/74

摘要： The disclosure relates to a recombinant membrane span protein complex, comprising (1) a fusion protein, comprising a membrane span protein fused to a kinase domain, preferably a constitutive kinase and (2) a reporter construct comprising a polypeptide, interacting with the membrane span protein, fused to a reporter phosphorylation domain. The disclosure relates further to the uses of such membrane span protein complex for the detection of compounds that interact with the membrane span protein and for the screening and/or detection of inhibitors of the compound-membrane span protein interactions. In a preferred embodiment, the membrane span protein is a G protein coupled receptor (GPCR) and the method is used for the screening and/or detection of inhibitors of the ligand-receptor binding.

公开(公告)号：US20190177705A1

公开(公告)日：2019-06-13

申请号：US16284999

申请日：2019-02-25

申请人： VIB VZW ,  UNIVERSITEIT GENT

发明人： Jan Tavernier ,  Samuel Lievens

IPC分类号： C12N9/12 ,  C12Q1/48 ,  G01N33/542 ,  C12N15/10 ,  C12N15/62

摘要： The disclosure relates to a cytoplasmic protein complex comprising: (a) a first recombinant fusion protein comprising a kinase, fused to a first interaction polypeptide; and (b) a second recombinant fusion protein comprising a domain comprising a reporter phosphorylation site, whereby the domain is fused to a second interaction polypeptide. The disclosure relates further to a method to detect compound-compound-interaction using the cytoplasmic protein complex, and to cells comprising such cytoplasmic protein complex.

公开(公告)号：US11053300B2

公开(公告)日：2021-07-06

申请号：US16444910

申请日：2019-06-18

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Samuel Lievens

IPC分类号： C07K14/72 ,  C07K14/705 ,  C12N9/12 ,  C12N15/62 ,  C12N9/02 ,  C12Q1/66 ,  G01N33/58 ,  G01N33/74

摘要： The disclosure relates to a recombinant membrane span protein complex, comprising (1) a fusion protein, comprising a membrane span protein fused to a kinase domain, preferably a constitutive kinase and (2) a reporter construct comprising a polypeptide, interacting with the membrane span protein, fused to a reporter phosphorylation domain. The disclosure relates further to the uses of such membrane span protein complex for the detection of compounds that interact with the membrane span protein and for the screening and/or detection of inhibitors of the compound-membrane span protein interactions. In a preferred embodiment, the membrane span protein is a G protein coupled receptor (GPCR) and the method is used for the screening and/or detection of inhibitors of the ligand-receptor binding.

公开(公告)号：US20190330310A1

公开(公告)日：2019-10-31

申请号：US16444910

申请日：2019-06-18

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Samuel Lievens

IPC分类号： C07K14/72 ,  C12Q1/66 ,  G01N33/74 ,  C07K14/705 ,  C12N9/12 ,  C12N15/62 ,  G01N33/58 ,  C12N9/02

摘要： The disclosure relates to a recombinant membrane span protein complex, comprising (1) a fusion protein, comprising a membrane span protein fused to a kinase domain, preferably a constitutive kinase and (2) a reporter construct comprising a polypeptide, interacting with the membrane span protein, fused to a reporter phosphorylation domain. The disclosure relates further to the uses of such membrane span protein complex for the detection of compounds that interact with the membrane span protein and for the screening and/or detection of inhibitors of the compound-membrane span protein interactions. In a preferred embodiment, the membrane span protein is a G protein coupled receptor (GPCR) and the method is used for the screening and/or detection of inhibitors of the ligand-receptor binding.

公开(公告)号：US20150011417A1

公开(公告)日：2015-01-08

申请号：US14381502

申请日：2013-03-06

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Samuel Lievens

IPC分类号： C07K14/72 ,  G01N33/58 ,  G01N33/74 ,  C12Q1/66 ,  C12N9/12 ,  C12N9/02

CPC分类号： C07K14/723 ,  C07K14/705 ,  C07K2319/42 ,  C07K2319/61 ,  C12N9/0069 ,  C12N9/12 ,  C12N15/62 ,  C12Q1/66 ,  C12Y207/10002 ,  G01N33/581 ,  G01N33/74 ,  G01N2333/726 ,  G01N2333/90241 ,  G01N2333/91205 ,  G01N2500/02 ,  G01N2500/10

摘要： The disclosure relates to a recombinant membrane span protein complex, comprising (1) a fusion protein, comprising a membrane span protein fused to a kinase domain, preferably a constitutive kinase and (2) a reporter construct comprising a polypeptide, interacting with the membrane span protein, fused to a reporter phosphorylation domain. The disclosure relates further to the uses of such membrane span protein complex for the detection of compounds that interact with the membrane span protein and for the screening and/or detection of inhibitors of the compound-membrane span protein interactions. In a preferred embodiment, the membrane span protein is a G protein coupled receptor (GPCR) and the method is used for the screening and/or detection of inhibitors of the ligand-receptor binding.

摘要翻译： 本公开涉及重组膜跨度蛋白复合物，其包含（1）融合蛋白，其包含与激酶结构域融合的膜跨度蛋白，优选组成型激酶，和（2）与膜跨度相互作用的包含多肽的报道构建体 蛋白质，与报告基因磷酸化结构域融合。 本公开进一步涉及这种膜跨度蛋白复合物用于检测与膜跨度蛋白相互作用的化合物以及用于筛选和/或检测化合物 - 膜跨度蛋白相互作用的抑制剂的用途。 在优选的实施方案中，膜跨度蛋白是G蛋白偶联受体（GPCR），并且该方法用于筛选和/或检测配体 - 受体结合的抑制剂。