公开(公告)号：US20190018928A1

公开(公告)日：2019-01-17

申请号：US16066846

申请日：2016-12-30

申请人： VITO NV ,  Universiteit Antwerpen

发明人： Dirk Valkenborg ,  Jef Hooyberghs ,  Kris Laukens

IPC分类号： G06F19/24 ,  G01N33/68 ,  C12Q1/6872 ,  G06F19/12 ,  H01J49/00

摘要： Methods and tools for determining the structure of biomacromolecules such as proteins in a sample using mass spectrometry. More particularly the methods allow determining the presence of a biomacromolecule of an organism in a sample by comparing an observed mass spectrum of the sample with a theoretical fragment ion spectrum comprising theoretical fragment ion masses.

公开(公告)号：US20170253919A1

公开(公告)日：2017-09-07

申请号：US15511366

申请日：2015-09-17

申请人： VITO NV

发明人： Jef Hooyberghs

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6837 ,  C12Q2537/165 ,  C12Q2545/101 ,  C12Q2565/101

摘要： Provided herein are methods for analyzing hybridization between a target polynucleotide in a sample solution and probes bound to a surface, such as probes of a microarray. The methods involve contacting said sample solution to at least two probe sets, which are characterized in that the probes within each probe set have a hybridization sequence of a fixed length. Probes of different probe sets have hybridization sequences of different lengths. A comparison between the data obtained for the different probe sets allows for increasing the dynamic range of hybridization methods.

公开(公告)号：US11378581B2

公开(公告)日：2022-07-05

申请号：US16343038

申请日：2017-10-20

申请人： VITO NV ,  Universiteit Antwerpen

发明人： Dirk Valkenborg ,  Jef Hooyberghs ,  Frank Sobott ,  Kris Laukens ,  Frederik Lermyte

IPC分类号： G01N33/68 ,  G16Z99/00 ,  H01J49/00

摘要： The present invention provides a method for the determination of the monoisotopic mass of a macromolecule from a mass Mmono spectrometry spectrum of said macromolecule based on the experimentally determined most abundant mass, with accuracy in the low parts-per-million (ppm) range. The method uses a simple, double-linear model for predicting the monoisotopic mass based on the experimentally determined most abundant mass, comprising the steps of (a) deriving the most abundant mass MMostAb from the spectrum; and (b) calculating the monoisotopic mass MMono from the most abundant mass MMostAb, using MMono=a+βMMOSTAB+ε; wherein β is a scalar slope obtainable by fitting the slope of monoisotopic mass versus most abundant mass for a plurality of macromolecules from a macromolecule database; and ε is a scalar residue of the form ε=εint+sfrac, εint being an integer, and εfrac being a sawtooth function of MMostAb.

公开(公告)号：US10598642B2

公开(公告)日：2020-03-24

申请号：US15362697

申请日：2016-11-28

申请人： VITO NV

发明人： Dirk Valkenborg ,  Jef Hooyberghs

IPC分类号： G01N33/487 ,  C40B30/02 ,  G01N30/88 ,  G16C20/70 ,  G01N30/72 ,  G16C20/20

摘要： A method is for the simultaneous identification of one or more chemical compounds contained in a sample, from an analytical measurement of a pool of two or more of the samples. A measured intensity of a first and second signal is representative of an abundance of respectively the first and second chemical compound in the first sample, and a measured intensity of a third and fourth second signal is representative of an abundance of respectively a third and fourth chemical compound in the second sample. The first and third, and the second and fourth compound may be the same or different. The signal intensities are organized in a matrix aij of m columns and n rows, in which n is ≥2 and corresponds to the number of chemical compounds in the pool, and m≥2 and corresponds to the number of samples in the pool.

公开(公告)号：US20190250168A1

公开(公告)日：2019-08-15

申请号：US16343038

申请日：2017-10-20

申请人： VITO NV ,  Universiteit Antwerpen

发明人： Dirk Valkenborg ,  Jef Hooyberghs ,  Frank Sobott ,  Kris Laukens ,  Frederik Lermyte

IPC分类号： G01N33/68 ,  H01J49/00

CPC分类号： G01N33/6848 ,  G01N2560/00 ,  G16Z99/00 ,  H01J49/0036

摘要： The present invention provides a method for the determination of the monoisotopic mass of a macromolecule from a mass Mmonospectrometry spectrum of said macromolecule based on the experimentally determined most abundant mass, with accuracy in the low parts-per-million (ppm) range. The method uses a simple, double-linear model for predicting the monoisotopic mass based on the experimentally determined most abundant mass, comprising the steps of (a) deriving the most abundant mass MMostAb from the spectrum; and (b) calculating the monoisotopic mass MMono from the most abundant mass MMostAb, using MMono=a+βMMOSTAB+ε; wherein β is a scalar slope obtainable by fitting the slope of monoisotopic mass versus most abundant mass for a plurality of macromolecules from a macromolecule database; and ε is a scalar residue of the form ε=εint+sfrac, εint being an integer, and εfrac being a sawtooth function of MMostAb.

公开(公告)号：US20170306391A1

公开(公告)日：2017-10-26

申请号：US15511380

申请日：2015-09-17

申请人： VITO NV

发明人： Jef Hooyberghs ,  An Jacobs ,  Hanny Willems

IPC分类号： C12Q1/68 ,  G06F19/18 ,  G06F19/20

CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q2525/161 ,  C12Q2565/501 ,  G16B20/00 ,  G16B25/00 ,  C12Q2537/165 ,  C12Q2545/101

摘要： Provided herein are methods for determining the presence of a mutant of target polynucleotides in a sample solution by contacting the sample solution and a reference solution containing the target nucleotide with identical probe sets and then comparing the hybridization intensities of corresponding probes of the probe sets. The probes provide a varying complementarity to the target sequence so that a range of hybridization intensities for the hybridization between the target polynucleotide and the probes is covered.