公开(公告)号：US20030059873A1

公开(公告)日：2003-03-27

申请号：US10029840

申请日：2001-12-31

Applicant: Virginia Tech Intellectual Properties, Inc.

Inventor： Xiang-Jin Meng ,  Gholamreza Haqshenas ,  Fang-Fang Huang

IPC: A61K031/70 ,  A01N043/04 ,  C12P021/06 ,  C12P019/34 ,  A61K039/12 ,  A61K039/29

CPC classification number: C07K14/005 ,  A61K2039/5254 ,  A61K2039/54 ,  C12N7/00 ,  C12N2770/28022 ,  C12N2770/28034 ,  C12Q1/707 ,  G01N33/576

Abstract: The present invention relates to a novel isolated avian hepatitis E virus having a nucleotide sequence set forth in SEQ ID NO: 1 or its complementary strand. The invention further concerns immunogenic compositions comprising this new virus or a recombinant products such as the nucleic acid and vaccines that protect an avian or mammalian species from viral infection or hepatitis-splenomegaly syndrome caused by the hepatitis E virus. Also included in the scope of the invention is a method for propagating, inactivating or attenuating a hepatitis E virus comprising inoculating an embryonated chicken egg with a live, pathogenic hepatitis E virus and recovering the virus or serially passing the pathogenic virus through additional embryonated chicken eggs until the virus is rendered inactivated or attenuated. Further, this invention concerns diagnostic reagents for detecting an avian hepatitis E viral infection or diagnosing hepatitis-splenomegaly syndrome in an avian or mammalian species comprising an antibody raised or produced against the immunogenic compositions and antigens such as ORF2 proteins expressed in a baculovirus vector, E. coli, etc. The invention additionally encompasses methods for detecting avian HEV nucleic acid sequences using nucleic acid hybridization probes or oligonucleotide primers for polymerase chain reaction (PCR).

Abstract translation: 本发明涉及具有SEQ ID NO：1所示核苷酸序列或其互补链的新颖的分离型禽类戊型肝炎病毒。 本发明还涉及包含该新病毒的免疫原性组合物或重组产物，例如保护禽类或哺乳动物物种免受由戊型肝炎病毒引起的病毒感染或肝炎 - 脾肿大综合征的核酸和疫苗。 本发明的范围还包括传播，灭活或减毒戊型肝炎病毒的方法，包括用活的致病性戊型肝炎病毒接种含胚鸡蛋，并回收该病毒或通过另外的含胚鸡蛋串联通过病原性病毒 直到病毒灭活或减毒。 此外，本发明涉及用于检测禽流感病毒感染或诊断禽流感或哺乳动物物种中的肝炎 - 脾肿大综合征的诊断试剂，其包含针对免疫原性组合物产生或产生的抗体，例如杆状病毒载体中表达的ORF2蛋白 大肠杆菌等。本发明另外包括使用核酸杂交探针或用于聚合酶链反应（PCR）的寡核苷酸引物检测禽类HEV核酸序列的方法。

公开(公告)号：US20030096377A1

公开(公告)日：2003-05-22

申请号：US10184191

申请日：2002-06-27

Applicant: Virginia Tech Intellectual Properties, Inc.

Inventor： Xiang-Jin Meng ,  Martijn Fenaux

IPC: C12Q001/70 ,  C12Q001/68 ,  C12P019/34

CPC classification number: C12Q1/701 ,  C12Q1/683

Abstract: The present invention relates to a method for detecting and differentiating PCV infections in a biological sample taken from a pig which involves amplifying a fragment from an extracted nucleic acid; digesting the fragment with a suitable restriction enzyme such as the unique NcoI restriction enzyme; forming a restriction fragment length polymorphism pattern; and then detecting the presence or absence of a PCV isolate. The invention further concerns the new oligonucleotide primers for differentiating PCV infections comprising a nucleotide sequence selected from the group consisting of MCV1 having a nucleotide sequence set forth in SEQ ID NO: 1 and MCV2 having a nucleotide sequence set forth in SEQ ID NO: 2. Moreover, this invention provides a novel kit for detecting and distinguishing PCV infections that includes the new oligonucleotide primers and the suitable restriction enzyme.

Abstract translation: 本发明涉及一种用于检测和分化取自猪的生物样品中的PCV感染的方法，其涉及从提取的核酸扩增片段; 用合适的限制酶如独特的NcoI限制酶消化片段; 形成限制性片段长度多态性模式; 然后检测PCV分离物的存在或不存在。 本发明还涉及用于分化PCV感染的新的寡核苷酸引物，其包含选自具有SEQ ID NO：1所示核苷酸序列的MCV1和具有SEQ ID NO：2所示核苷酸序列的MCV2的核苷酸序列。 此外，本发明提供了用于检测和鉴别PCV感染的新型试剂盒，其包括新的寡核苷酸引物和合适的限制酶。

公开(公告)号：US20040253270A1

公开(公告)日：2004-12-16

申请号：US10808964

申请日：2004-03-25

Applicant: Virginia Tech Intellectual Properties, Inc. ,  Iowa State University Research Foundation, Inc.

Inventor： Xiang-Jin Meng ,  Martijn Fenaux ,  Patrick G. Halbur

IPC: A61K039/12 ,  C07H021/04 ,  C12N007/00

CPC classification number: A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10061

Abstract: The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products. In addition, the invention encompasses two mutations in the PCV2 immunogenic capsid gene and protein, and the introduction of the ORF2 mutations in the chimeric clones.

Abstract translation: 本发明涉及感染性DNA克隆，猪圆环病毒感染性嵌合DNA克隆（PCV），疫苗和保护猪免受病毒感染或PCV2引起的断奶后多系统消耗综合征（PMWS）的方法。 新的嵌合感染性DNA克隆及其衍生的无毒性嵌合病毒由非致病性PCV1构建，其中致病性PCV2的免疫原性ORF基因替代非致病性PCV1的基因，优选在相同的位置。 嵌合病毒有利地保留了PCV1的非病原性表型，但引发针对致病性PCV2的特异性免疫应答。 本发明还包括免疫原性多肽表达产物。 此外，本发明包括PCV2免疫原性衣壳蛋白基因和蛋白质中的两个突变，以及在嵌合克隆中引入ORF2突变。

公开(公告)号：US20030170270A1

公开(公告)日：2003-09-11

申请号：US10314512

申请日：2002-12-09

Applicant: Virginia Tech Intellectual Properties, Inc.

Inventor： Xiang-Jin Meng ,  Martijn Fenaux ,  Patrick G. Halbur

IPC: C12P021/06 ,  A61K048/00 ,  A01N063/00 ,  A61K039/12 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74

CPC classification number: C07K14/005 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10061

Abstract: The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products.

Abstract translation: 本发明涉及感染性DNA克隆，猪圆环病毒感染性嵌合DNA克隆（PCV），疫苗和保护猪免受病毒感染或PCV2引起的断奶后多系统消耗综合征（PMWS）的方法。 新的嵌合感染性DNA克隆及其衍生的无毒性嵌合病毒由非致病性PCV1构建，其中病原性PCV2的免疫原性ORF基因替代非致病性PCV1的基因，优选在相同的位置。 嵌合病毒有利地保留了PCV1的非病原性表型，但引发针对致病性PCV2的特异性免疫应答。 本发明还包括免疫原性多肽表达产物。