-
公开(公告)号：US10526367B2
公开(公告)日：2020-01-07
申请号：US15409733
申请日：2017-01-19
IPC分类号： C07K1/22 , B01D15/22 , C07K16/00 , B01D15/38 , B01J20/28 , B01J20/286 , B01D63/08 , B01D63/10 , B01J20/10 , B01D69/14
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include (1) at least one polymer membrane that contains therein inorganic particles and (2) at least one impermeable layer (e.g., a thermoplastic polymer in a solid state). The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices has a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
-
公开(公告)号：US10974170B2
公开(公告)日：2021-04-13
申请号：US16689677
申请日：2019-11-20
发明人： Michael C. McManaway
IPC分类号： B01D15/38 , B01J20/286 , B01J20/28 , B01D15/22 , B01D69/14 , B01J20/06 , B01J20/26 , B01J20/10 , B01D63/08 , B01D63/10 , B01D69/02 , C07K1/22 , C07K16/00
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
-
公开(公告)号：US10525376B2
公开(公告)日：2020-01-07
申请号：US15094428
申请日：2016-04-08
发明人： Michael C. McManaway
IPC分类号： B01D15/38 , C07K1/22 , B01D69/02 , C07K16/00 , B01D63/08 , B01D63/10 , B01D15/22 , B01J20/28 , B01J20/286 , B01J20/06 , B01J20/26 , B01D69/14 , B01J20/10
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
-
公开(公告)号：US20200086233A1
公开(公告)日：2020-03-19
申请号：US16689677
申请日：2019-11-20
发明人： Michael C. McManaway
IPC分类号： B01D15/38 , C07K16/00 , C07K1/22 , B01D69/02 , B01D63/10 , B01D63/08 , B01J20/28 , B01J20/10 , B01J20/26 , B01J20/06 , B01D69/14 , B01D15/22 , B01J20/286
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
-
公开(公告)号：US20170021286A1
公开(公告)日：2017-01-26
申请号：US15094428
申请日：2016-04-08
发明人： Michael C. McManaway
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
摘要翻译： 本发明涉及从含有目标蛋白质或抗体的水性混合物分离靶向蛋白质或抗体的亲和层析装置。 色谱装置可以包含层叠膜组件或缠绕膜组件。 膜组件包括至少一个在其中含有无机颗粒的聚合物膜。 聚合物膜和/或无机颗粒具有与其结合的亲和配体。 亲和配体可以是可逆结合靶向蛋白质或抗体的蛋白质，抗体或多糖。 色谱装置可以重复使用，并且可以在使用之间用苛性碱溶液清洗。 在20秒或更短的停留时间下，色谱装置可以具有至少30mg / ml（或0.07微摩尔/ ml）的10％穿透的动态结合能力（DBC）。
-
公开(公告)号：US20220032209A1
公开(公告)日：2022-02-03
申请号：US17290985
申请日：2018-11-05
IPC分类号： B01D15/38 , B01D15/22 , B01D63/10 , B01D71/36 , B01D71/02 , B01D69/14 , C07K1/22 , G01N30/38 , G01N30/60 , G01N30/88
摘要： The present invention is directed to an affinity chromatography device that has a normal flow and which separates a targeted protein from aqueous mixtures. The chromatography device includes a housing containing therein a spiral wound membrane assembly that includes at least one inner intermediate material that forms an outer flow channel, at least one polymer membrane that contains therein inorganic particles, and at least one outer intermediate material that forms an inner flow channel sequentially positioned around a central core having a solid outer wall. An aqueous mixture is passed through the outer flow channel, through the polymer membrane where the targeted protein is removed, and then through an inner flow channel. The affinity chromatography device further includes an inlet flow distributor containing an inlet and an outlet flow distributer containing an outlet. Additionally, the chromatography device has a dimensionless resistance parameter that is less than 0.08.
-
公开(公告)号：US10974169B2
公开(公告)日：2021-04-13
申请号：US16689630
申请日：2019-11-20
发明人： Michael C. McManaway
IPC分类号： B01D15/38 , B01J20/286 , B01J20/28 , B01D15/22 , B01D69/14 , B01J20/06 , B01J20/26 , B01J20/10 , B01D63/08 , B01D63/10 , B01D69/02 , C07K1/22 , C07K16/00
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
-
公开(公告)号：US20200086232A1
公开(公告)日：2020-03-19
申请号：US16689630
申请日：2019-11-20
发明人： Michael C. McManaway
IPC分类号： B01D15/38 , B01J20/286 , B01J20/28 , B01D15/22 , B01D69/14 , B01J20/06 , B01J20/26 , B01J20/10 , B01D63/08 , B01D63/10 , B01D69/02 , C07K1/22 , C07K16/00
摘要： The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.023 秒)
