公开(公告)号：US07116407B2

公开(公告)日：2006-10-03

申请号：US10076363

申请日：2002-02-15

申请人： W. Peter Hansen ,  Anthony A. Ferrante ,  Russell J. Gershman ,  Petra B. Krauledat ,  Donald F. Perrault, Jr.

发明人： W. Peter Hansen ,  Anthony A. Ferrante ,  Russell J. Gershman ,  Petra B. Krauledat ,  Donald F. Perrault, Jr.

IPC分类号： G01N21/00 ,  G01N33/553 ,  B07C5/36

CPC分类号： G01N33/5085 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N2015/145 ,  G01N2015/1477 ,  G01N2015/149 ,  G01N2015/1497

摘要： A method of using elongate multicellular organisms in conjunction with a specialized flow cytometer for drug discovery and compound screening. A stable, optically detectable linear marker pattern on each organism is used to construct a longitudinal map of each organism as it passes through the analysis region of the flow cytometer. This pattern is used to limit complex data analysis to particular regions of each organism thereby simplifying and speeding analysis. The longitudinal marker pattern can be used to alter signal detection modes at known regions of the organism to enhance sensitivity and overall detection effectiveness. A repeating pattern can also be used to add a synchronous element to data analysis. The marker patterns are established using known methods of molecular biology to express various indicator molecules. Inherent features of the organism can be rendered detectable to serve as marker patterns.

公开(公告)号：US06320656B1

公开(公告)日：2001-11-20

申请号：US09507515

申请日：2000-02-18

申请人： Anthony A. Ferrante ,  W. Peter Hansen

发明人： Anthony A. Ferrante ,  W. Peter Hansen

IPC分类号： G01N2100

CPC分类号： G01N15/1456 ,  G01N15/1434 ,  G01N2015/0065 ,  G01N2021/4704

摘要： The high numerical aperture flow cytometer of the present invention includes a flow cell and a laser input. The laser input emits a beam of light that is oriented substantially orthoganilly to the flow of blood cells through the flow cell such that laser light impinges upon the blood cells as they pass through the flow cell. A portion of the beam from the laser input that impinges upon the blood cells in the flow cell is scattered at a substantially right angle to the beam of laser input (“right angle scatter”). A second portion of the beam from the laser input that impinges upon the cells in the flow cell is scattered at a much lower angle than 90°. This scatter is termed “low angle forward scatter light” and has an angle of from about 2° to about 5° from the orientation of the original beam from laser input. A right angle scatter light detector is oriented to receive the previously mentioned right angle scatter light. A low angle forward scatter light detector is oriented to capture the previously mentioned low angled forward scatter light oriented at about 2° to about 5° from the beam of the laser input.

摘要翻译： 本发明的高数值孔径流式细胞仪包括流动池和激光输入。 激光输入发射光束，该光束基本上与血细胞流动通过流动池流动正交，使得激光在穿过流动池时撞击血细胞。 来自激光输入的撞击流通池中的血细胞的光束的一部分以与激光束输入（“直角散射”）基本成直角的角度散射。 来自激光输入的撞击流通池中的电池的光束的第二部分以比90°更低的角度散射。 这种散射被称为“低角度前向散射光”，并且与原始光束从激光输入的方向成约2°至约5°的角度。 直角散射光检测器被定向为接收前述直角散射光。 低角度前向散射光检测器被定向以捕获从激光输入的光束约2°至约5°定向的前述低角度前向散射光。