公开(公告)号：US11826757B2

公开(公告)日：2023-11-28

申请号：US16635703

申请日：2018-08-01

Applicant: Wake Forest University Health Sciences

Inventor： Christopher Daniel Porada ,  Aleksander Skardal ,  Maria Graca Almeida-Porada

IPC: C12M3/06 ,  B01L3/00 ,  C12M3/00 ,  C12M1/00

CPC classification number: B01L3/502753 ,  B01L3/502715 ,  C12M21/08 ,  C12M23/16 ,  C12M27/18 ,  B01L2200/10 ,  B01L2300/0861

Abstract: Provided herein according to some embodiments is an in vitro construct useful as a model for a hematopoietic microenvironment, which may include: a microfluidic device having multiple chambers; and two or more populations of cells (e.g., 3 or 4 populations of cells) (or “niches”) selected from: 1) mesenchymal cells (e.g., Stro-1+; MSC); 2) osteoblasts (OB; optionally said osteoblasts provided by differentiating mesenchymal cells to differentiated osteoblasts); 3) arterial endothelium (e.g., CD146+NG2+; AEC); and 4) sinusoidal endothelium (CD146+NG2−; SEC), wherein each of said two or more populations of cells are provided in a separate chamber of the microfluidic device. Methods of making and using the construct are also provided.

公开(公告)号：US10913933B2

公开(公告)日：2021-02-09

申请号：US14767335

申请日：2014-02-13

Applicant: Wake Forest University Health Sciences

Inventor： Maria Graca Almeida-Porada ,  Pedro Miguel A. M. Baptista ,  Shay Soker

IPC: C12N5/071 ,  C12N5/0789 ,  C12N5/00

Abstract: An in vitro liver organoid is provided along with methods of making and using the organoid. A cell culture system that includes the liver organoid is also provided. The liver organoid has fetal liver characteristics and supports expansion and differentiation of hematopoietic stem cells.

公开(公告)号：US20160002602A1

公开(公告)日：2016-01-07

申请号：US14767335

申请日：2014-02-13

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Maria Graca Almeida-Porada ,  Pedro Miguel A.M. Baptista ,  Shay Soker

IPC: C12N5/071 ,  C12N5/0789

CPC classification number: C12N5/0671 ,  C12N5/0068 ,  C12N5/0647 ,  C12N5/067 ,  C12N2501/01 ,  C12N2501/04 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/125 ,  C12N2501/235 ,  C12N2501/26 ,  C12N2501/30 ,  C12N2501/305 ,  C12N2501/315 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2533/90

Abstract: An in vitro liver organoid is provided along with methods of making and using the organoid. The liver organoid can be manipulated to have either immature or mature characteristics. Some manipulations generate a liver organoid that supports expansion and/or differentiation of hematopoietic stem cells.

Abstract translation: 提供了一种体外肝组织细胞以及制造和使用有机体的方法。 肝组织细胞可以被操纵以具有未成熟或成熟的特征。 一些操作产生支持造血干细胞的扩增和/或分化的肝组织。