公开(公告)号：US11648335B2

公开(公告)日：2023-05-16

申请号：US14610902

申请日：2015-01-30

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Giuseppe Orlando ,  Shay Soker

IPC: A61L27/36 ,  A61L27/38 ,  A61L27/54

CPC classification number: A61L27/3633 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/256 ,  A61L2300/42 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/40

Abstract: Methods for decellularizing organs and tissues in vitro and in vivo are provided, as are methods of maintaining organ and tissue frameworks and methods of recellularizing organs and tissues, thereby providing an approach to needed organs or tissues.

公开(公告)号：US09707256B2

公开(公告)日：2017-07-18

申请号：US14102953

申请日：2013-12-11

Applicant: Wake Forest University Health Sciences

Inventor： Shay Soker ,  J. Koudy Williams ,  Patrick Laber ,  Margaret Greven ,  Keith A. Walter

IPC: A61K35/28 ,  A61K35/30 ,  A61L27/54 ,  A61K35/44 ,  A61F2/14 ,  A61K9/00 ,  A61L27/36 ,  A61L27/38 ,  A61K35/12

CPC classification number: A61K35/44 ,  A61F2/142 ,  A61K9/0051 ,  A61K35/12 ,  A61L27/3604 ,  A61L27/3808 ,  A61L2430/16

Abstract: Provided herein is an endothelial scaffold comprising, consisting of, or consisting essentially of decellularized corneal stroma. In some embodiments, the scaffold has cultured endothelial cells seeded thereon. Methods of treating a patient in need of corneal endothelial transplant are also provided, including implanting the scaffold as described herein onto a cornea of the patient (e.g., by deep keratectomy).

公开(公告)号：US10913933B2

公开(公告)日：2021-02-09

申请号：US14767335

申请日：2014-02-13

Applicant: Wake Forest University Health Sciences

Inventor： Maria Graca Almeida-Porada ,  Pedro Miguel A. M. Baptista ,  Shay Soker

IPC: C12N5/071 ,  C12N5/0789 ,  C12N5/00

Abstract: An in vitro liver organoid is provided along with methods of making and using the organoid. A cell culture system that includes the liver organoid is also provided. The liver organoid has fetal liver characteristics and supports expansion and differentiation of hematopoietic stem cells.

公开(公告)号：US20160002602A1

公开(公告)日：2016-01-07

申请号：US14767335

申请日：2014-02-13

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Maria Graca Almeida-Porada ,  Pedro Miguel A.M. Baptista ,  Shay Soker

IPC: C12N5/071 ,  C12N5/0789

CPC classification number: C12N5/0671 ,  C12N5/0068 ,  C12N5/0647 ,  C12N5/067 ,  C12N2501/01 ,  C12N2501/04 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/125 ,  C12N2501/235 ,  C12N2501/26 ,  C12N2501/30 ,  C12N2501/305 ,  C12N2501/315 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2533/90

Abstract: An in vitro liver organoid is provided along with methods of making and using the organoid. The liver organoid can be manipulated to have either immature or mature characteristics. Some manipulations generate a liver organoid that supports expansion and/or differentiation of hematopoietic stem cells.

Abstract translation: 提供了一种体外肝组织细胞以及制造和使用有机体的方法。 肝组织细胞可以被操纵以具有未成熟或成熟的特征。 一些操作产生支持造血干细胞的扩增和/或分化的肝组织。

公开(公告)号：US20140377326A1

公开(公告)日：2014-12-25

申请号：US14344011

申请日：2012-09-17

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： GuoGuang Niu ,  Shay Soker

IPC: A61K35/30

CPC classification number: A61K35/30 ,  A61F2/142 ,  A61L27/222 ,  A61L27/26 ,  A61L27/3808 ,  A61L27/50 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/236 ,  A61L2430/16 ,  C08L89/06 ,  C08L5/08 ,  C08L71/02

Abstract: The invention provides a corneal endothelial composition comprising a transparent hydrogel scaffold and a single layer of cultured corneal endothelial cells on the surface of the scaffold. The hydrogel scaffold I comprised of at least one biopolymer, preferably gelatin. Also provided are methods of making a corneal endothelial scaffold

Abstract translation: 本发明提供角膜内皮组合物，其包含透明水凝胶支架和在支架表面上的单层培养的角膜内皮细胞。 水凝胶支架I由至少一种生物聚合物组成，优选明胶。 还提供了制造角膜内皮支架的方法

公开(公告)号：US20140099290A1

公开(公告)日：2014-04-10

申请号：US14102953

申请日：2013-12-11

Applicant: Wake Forest University Health Sciences

Inventor： Shay Soker ,  J. Koudy Williams ,  Patrick Laber ,  Margaret Greven ,  Keith A. Walter

IPC: A61K35/44

CPC classification number: A61K35/44 ,  A61F2/142 ,  A61K9/0051 ,  A61K35/12 ,  A61L27/3604 ,  A61L27/3808 ,  A61L2430/16

Abstract: Provided herein is an endothelial scaffold comprising, consisting of, or consisting essentially of decellularized corneal stroma. In some embodiments, the scaffold has cultured endothelial cells seeded thereon. Methods of treating a patient in need of corneal endothelial transplant are also provided, including implanting the scaffold as described herein onto a cornea of the patient (e.g., by deep keratectomy).

Abstract translation: 本文提供的是包含脱细胞角膜基质的或由其组成的内皮支架。 在一些实施方案中，支架具有接种于其上的培养的内皮细胞。 还提供了治疗需要角膜内皮移植的患者的方法，包括将本文所述的支架植入患者的角膜（例如，通过深部角膜切除术）。

公开(公告)号：US10052350B2

公开(公告)日：2018-08-21

申请号：US14344011

申请日：2012-09-17

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： GuoGuang Niu ,  Shay Soker

IPC: A61K35/30 ,  A61L27/22 ,  A61L27/26 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61F2/14

CPC classification number: A61K35/30 ,  A61F2/142 ,  A61L27/222 ,  A61L27/26 ,  A61L27/3808 ,  A61L27/50 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/236 ,  A61L2430/16 ,  C08L89/06 ,  C08L5/08 ,  C08L71/02

Abstract: The invention provides a corneal endothelial composition comprising a transparent hydrogel scaffold and a single layer of cultured corneal endothelial cells on the surface of the scaffold. The hydrogel scaffold I comprised of at least one biopolymer, preferably gelatin. Also provided are methods of making a corneal endothelial scaffold.

公开(公告)号：US20150247194A1

公开(公告)日：2015-09-03

申请号：US14712395

申请日：2015-05-14

Applicant: Wake Forest University Health Sciences

Inventor： Jason Hipp ,  Shay Soker

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present invention concerns methods of screening cells for differentiation or de-differentiation, and/or for status as a pluripotent or multipotent (e.g., “stem”) cell, by detecting the differential expression (e.g., upregulation, downregulation) of genes.

Abstract translation: 本发明涉及通过检测基因的差异表达（例如上调，下调）筛选用于分化或去分化的细胞和/或作为多潜能或多能（例如“干”）细胞的状态的方法。

公开(公告)号：US20150238656A1

公开(公告)日：2015-08-27

申请号：US14610902

申请日：2015-01-30

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Giuseppe Orlando ,  Shay Soker

IPC: A61L27/36 ,  A61L27/54 ,  A61L27/38

CPC classification number: A61L27/3633 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/256 ,  A61L2300/42 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/40

Abstract: Methods for decellularizing organs and tissues in vitro and in vivo are provided, as are methods of maintaining organ and tissue frameworks and methods of recellularizing organs and tissues, thereby providing an approach to needed organs or tissues.

Abstract translation: 提供体外和体内脱细胞化器官和组织的方法，以及维持器官和组织框架的方法以及使器官和组织接收的方法，从而提供所需器官或组织的方法。

公开(公告)号：US20230417740A1

公开(公告)日：2023-12-28

申请号：US18319832

申请日：2023-05-18

Applicant: Wake Forest University Health Sciences

Inventor： Aleksander Skardal ,  Shay Soker

IPC: G01N33/50 ,  C08H8/00 ,  C08B37/00 ,  C08H7/00 ,  B33Y70/00 ,  B29C64/106 ,  B01L3/00 ,  C12N5/071

CPC classification number: G01N33/5082 ,  C08H8/00 ,  C08B37/0057 ,  C08H6/00 ,  B33Y10/00 ,  B29C64/106 ,  B01L3/5027 ,  C12N5/0671 ,  G01N33/5014 ,  B33Y70/00

Abstract: An extrudable hydrogel composition useful for making a three-dimensional organ construct includes a cross-linkable prepolymer, a post-deposition crosslinking group, optionally, an initiator that catalyzes the reaction between the prepolymer and said the crosslinking group; live cells (e.g., plant, animal, or microbial cells), optionally at least one growth factor, and optionally water to balance. Methods of using the same and products so made are also described.