公开(公告)号：US20160206665A1

公开(公告)日：2016-07-21

申请号：US15014536

申请日：2016-02-03

Applicant: Wake Forest University Health Sciences

Inventor： Emmanuel Opara ,  James J. Yoo ,  Justin M. Saul ,  Sittadjody Sivanandane ,  Anthony Atala

IPC: A61K35/54 ,  A61K9/50

CPC classification number: A61K35/54 ,  A61K9/167 ,  A61K9/5036 ,  A61K9/5073 ,  A61K31/565 ,  A61K31/57 ,  A61K2300/00

Abstract: A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.

Abstract translation: 描述了包含微胶囊的组合物，包含活哺乳动物卵巢颗粒细胞和活哺乳动物卵巢细胞的微胶囊。 在一些实施方案中，颗粒细胞和细胞被包含在组合物中的分离的微胶囊中; 在一些实施方案中，颗粒细胞和细胞在组合物中一起包含在相同的微胶囊中。组合物可用于雌激素，并且任选地也可用于孕酮，递送，因此优选是游离的或基本上不含卵母细胞。 还描述了使用它们的方法和含有它们的药物制剂。

公开(公告)号：US20220010270A1

公开(公告)日：2022-01-13

申请号：US17449015

申请日：2021-09-27

Applicant: Wake Forest University Health Sciences

Inventor： Sittadjody Sivanandane ,  Russel C. Sequeira ,  John D. Jackson ,  Anthony Atala ,  James J. Yoo

IPC: C12N5/075

Abstract: A method of providing a culture of oogonia stem cells comprising oogonia stem cells is provided. The method may further include culturing the oogonia stem cells with granulosa and theca cells to differentiate the oogonia stem cells into oocytes. In some embodiments, the culturing comprises including the oogonia stem cells in an in vitro follicle construct or a microcapsule comprising said granulosa and theca cells. Further described herein is a method of forming a bioengineered follicle construct capable of releasing a mature oocyte. An in vitro fertilization method using the mature oocyte is also provided.

公开(公告)号：US09763986B2

公开(公告)日：2017-09-19

申请号：US15014536

申请日：2016-02-03

Applicant: Wake Forest University Health Sciences

Inventor： Emmanuel Opara ,  James J. Yoo ,  Justin M. Saul ,  Sittadjody Sivanandane ,  Anthony Atala

IPC: C12N5/07 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  A61K35/54 ,  A61K31/565 ,  A61K31/57 ,  A61K9/16 ,  A61K9/50

CPC classification number: A61K35/54 ,  A61K9/167 ,  A61K9/5036 ,  A61K9/5073 ,  A61K31/565 ,  A61K31/57 ,  A61K2300/00

Abstract: A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.

公开(公告)号：US10398739B2

公开(公告)日：2019-09-03

申请号：US14909240

申请日：2014-09-02

Applicant: Wake Forest University Health Sciences

Inventor： Emmanuel C. Opara ,  James J. Yoo ,  Justin M. Saul ,  Sittadjody Sivanandane ,  Anthony Atala

IPC: A61K35/54 ,  A61K47/36 ,  A61K9/50 ,  A61K35/28 ,  A61K31/57 ,  A61K31/565

Abstract: A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition. The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.

公开(公告)号：US20160166620A1

公开(公告)日：2016-06-16

申请号：US14909240

申请日：2014-09-02

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Emmanuel C. Opara ,  James J. Yoo ,  Justin M. Saul ,  Sittadjody Sivanandane ,  Anthony Atala

IPC: A61K35/54 ,  A61K31/565 ,  A61K9/50 ,  A61K35/28

CPC classification number: A61K35/54 ,  A61K9/5036 ,  A61K9/5052 ,  A61K9/5073 ,  A61K31/565 ,  A61K31/57 ,  A61K35/28 ,  A61K47/36

Abstract: A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition. The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.

Abstract translation: 描述了包含微胶囊的组合物，包含活哺乳动物卵巢颗粒细胞和活哺乳动物卵巢细胞的微胶囊。 在一些实施方案中，颗粒细胞和细胞被包含在组合物中的分离的微胶囊中; 在一些实施方案中，颗粒细胞和细胞在组合物中一起包含在相同的微胶囊中。 该组合物可用于雌激素，并且任选地也可用于孕酮，递送，因此优选为游离或基本上不含卵母细胞。 还描述了使用它们的方法和含有它们的药物制剂。