公开(公告)号：US5468616A

公开(公告)日：1995-11-21

申请号：US58692

申请日：1993-04-29

申请人： Wallace H. Coulter ,  John D. Hollinger ,  Kenneth H. Kortright ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

发明人： Wallace H. Coulter ,  John D. Hollinger ,  Kenneth H. Kortright ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

IPC分类号： G01N33/50 ,  G01N33/569 ,  C12Q1/24 ,  G01N33/543

CPC分类号： G01N33/5005 ,  G01N33/56966 ,  G01N33/56972 ,  Y10S436/808

摘要： A method and apparatus for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

摘要翻译： 一种用于加速至少一种确定性生物反应的方法和装置，包括增加由其制备的测定精度。 该反应涉及选择的活体生物细胞，它们以小的样品体积制备，并且与具有抗体特异性至少与结合于其上的特定细胞的微球快速混合。 微球可以是磁性的，并且结合的细胞可以被磁性去除以分析剩余的血细胞群体。 微球可以顺序或同时引入。

公开(公告)号：US5238812A

公开(公告)日：1993-08-24

申请号：US517309

申请日：1990-05-01

申请人： Wallace H. Coulter ,  John D. Hollinger ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

发明人： Wallace H. Coulter ,  John D. Hollinger ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

IPC分类号： G01N33/50 ,  G01N33/569

CPC分类号： G01N33/5005 ,  G01N33/56966 ,  G01N33/56972 ,  Y10S436/808

摘要： A method and apparatus for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

摘要翻译： 一种用于加速至少一种确定性生物反应的方法和装置，包括增加由其制备的测定精度。 该反应涉及选择的活体生物细胞，它们以小的样品体积制备，并且与具有抗体特异性至少与结合于其上的特定细胞的微球快速混合。 微球可以是磁性的，并且结合的细胞可以被磁性去除以分析剩余的血细胞群体。 微球可以顺序或同时引入。

公开(公告)号：US4515274A

公开(公告)日：1985-05-07

申请号：US326734

申请日：1981-12-02

申请人： John D. Hollinger ,  Raul I. Pedroso

发明人： John D. Hollinger ,  Raul I. Pedroso

IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  B07C5/02

CPC分类号： G01N15/1218 ,  G01N15/12 ,  G01N2015/1037 ,  G01N2015/1081 ,  G01N2015/149 ,  Y10S209/906

摘要： Disclosed is a flow-through, particle analyzer and sorter apparatus for simultaneous optical and electrical impedance measurements on a stream of particles, comprising a flow cell having a pair of channels fluidly connected by a particle sensing aperture, through which the particles pass and are analyzed; a nozzle mounted at the end of the downstream channel so as to define a flow chamber; a sheath liquid which is introduced at the bottom of the flow chamber to hydrodynamically focus the particle stream and to jet the same in a liquid jet from the nozzle; and a system for creating droplets from the liquid jet and for thereafter sorting the droplets.

摘要翻译： 公开了一种用于在颗粒物流上同时进行光和电阻抗测量的流通，粒子分析器和分选器装置，其包括具有通过粒子感测孔流体连接的一对通道的流动池，粒子通过该流通池并被分析 ; 安装在所述下游通道的端部处以限定流动室的喷嘴; 鞘液被引入流动室的底部以流体动力地聚焦颗粒物流并从喷嘴以液体射流喷射; 以及用于从液体射流产生液滴并随后对液滴进行分选的系统。

公开(公告)号：US5542305A

公开(公告)日：1996-08-06

申请号：US508596

申请日：1995-07-28

申请人： John D. Hollinger

发明人： John D. Hollinger

IPC分类号： G01F11/42 ,  G01N1/00 ,  G01N15/14 ,  G01N33/49

CPC分类号： G01N35/1097 ,  G01N15/1404

摘要： A metering and transfer valve assembly particularly for use in particle analyzing apparatus for the study of particles in liquid suspension, the valve assembly having at least pair of inner and outer valve elements coaxially arranged in face to face frictional sealing engagement with a center valve element, each of the valve elements being independently rotatable, first passages defining a first flow path through the assembly, second passages defining a second flow path through the assembly, the first passages including a metering chamber for measuring and transferring a precise sample aliquot of sample flowing in the first flow path into the second flow path, the first flow path leading to a first exterior location and the second flow path leading to a second exterior location, and a drive fluid being introduced to the second flow path subsequent to transfer of said aliquot thereto for propelling said aliquot along the second flow path to the second exterior destination, the second destination being a flow scanning cell whereby an absolute count of the full content of said aliquot can be established. The first destination can be a flow scanning cell and the first flow path can lead liquid sample to said flow scanning cell before the aliquot is transferred to said path. A method of cytometric analysis is described as well as apparatus utilizing said valve assembly.

摘要翻译： 一种特别用于研究液体悬浮液中的颗粒的颗粒分析装置的计量和转移阀组件，该阀组件具有至少一对同心地布置成与中心阀元件摩擦密封接合的内外阀元件， 每个阀元件可独立地旋转，第一通道限定穿过组件的第一流动路径，第二通道限定通过组件的第二流动路径，第一通道包括用于测量和转移精确样品等分试样的流量的计量室 进入第二流动路径的第一流动路径，通向第一外部位置的第一流动路径和通向第二外部位置的第二流动路径，以及在将所述等分试样转移到第二流动路径之后引入第二流动路径的驱动流体 用于将所述等分试样沿着第二流动路径推进到第二外部目的地， 排列为流动扫描单元，由此能够建立所述等分试样的全部内容的绝对计数。 第一目的地可以是流动扫描单元，并且在将等分试样转移到所述路径之前，第一流路可将液体样品引导到所述流动扫描单元。 描述了细胞分析的方法以及利用所述阀组件的装置。

公开(公告)号：US4504248A

公开(公告)日：1985-03-12

申请号：US423539

申请日：1982-09-27

申请人： John D. Hollinger

发明人： John D. Hollinger

IPC分类号： F16H15/42 ,  F16H55/56

CPC分类号： F16H15/42

摘要： A variable and reversible transmission, in which two co-linear shafts are provided with adjustably-locatable generally semi-spherical drive components or heads, with control means for changing and holding selected adjustments of the heads; and the heads drivingly engage one another, in whatever settings they are positioned, by acting through a pulley belt which may be either an idler for only transmitting torque from one head directly to the other, or may be a power member transferring power to another set of such heads, achieving both variability and reversibility of drive.

摘要翻译： 一个可变和可逆的变速器，其中两个共线轴设置有可调节定位的大致半球面的驱动部件或头部，其中控制装置用于改变和保持头部的选定调节; 并且头部通过作用于皮带轮的任何设置而彼此驱动地彼此接合，该皮带轮可以是仅将一个头部的扭矩传递到另一个的惰轮，或者可以是将动力传递到另一个组件的动力部件 的这样的头，实现驱动器的可变性和可逆性。

公开(公告)号：US5437200A

公开(公告)日：1995-08-01

申请号：US4812

申请日：1993-01-15

申请人： John D. Hollinger

发明人： John D. Hollinger

IPC分类号： G01F11/42 ,  G01N1/00 ,  G01N15/14 ,  G01N33/49 ,  G01N1/30

CPC分类号： G01N35/1097 ,  G01N15/1404

摘要： A metering and transfer valve assembly particularly for use in particle analyzing apparatus for the study of particles in liquid suspension, the valve assembly having at least pair of inner and outer valve elements coaxially arranged in face to face frictional sealing engagement with a center valve element, each of the valve elements being independently rotatable, first passages defining a first flow path through the assembly, second passages defining a second flow path through the assembly, the first passages including a metering chamber for measuring and transferring a precise sample aliquot of sample flowing in the first flow path into the second flow path, the first flow path leading to a first exterior location and the second flow path leading to a second exterior location, and a drive fluid being introduced to the second flow path subsequent to transfer of said aliquot thereto for propelling said aliquot along the second flow path to the second esterior destination, the second destination being a flow scanning cell whereby an absolute count of the full content of said aliquot can be established. The first destination can be a flow scanning cell and the first flow path can lead liquid sample to said flow scanning cell before the aliquot is transferred to said path. A method of cytometric analysis is described as well as apparatus utilizing said valve assembly.

摘要翻译： 一种特别用于研究液体悬浮液中的颗粒的颗粒分析装置的计量和转移阀组件，该阀组件具有至少一对同心地布置成与中心阀元件摩擦密封接合的内外阀元件， 每个阀元件可独立地旋转，第一通道限定穿过组件的第一流动路径，第二通道限定穿过组件的第二流动路径，第一通道包括用于测量和转移精确样品等分试样的流量的计量室 进入第二流动路径的第一流动路径，通向第一外部位置的第一流动路径和通向第二外部位置的第二流动路径，以及在将所述等分试样转移到其之后引入第二流动路径的驱动流体 用于将所述等分试样沿着第二流动路径推进到第二酯化物目的地， 排列为流动扫描单元，由此能够建立所述等分试样的全部内容的绝对计数。 第一目的地可以是流动扫描单元，并且在将等分试样转移到所述路径之前，第一流路可将液体样品引导到所述流动扫描单元。 描述了细胞分析的方法以及利用所述阀组件的装置。

公开(公告)号：US4845025A

公开(公告)日：1989-07-04

申请号：US119018

申请日：1987-11-10

申请人： Todd P. Lary ,  Jorge A. Quintana ,  Osvaldo E. Miranda ,  John R. DeChristopher, Sr. ,  John D. Hollinger

发明人： Todd P. Lary ,  Jorge A. Quintana ,  Osvaldo E. Miranda ,  John R. DeChristopher, Sr. ,  John D. Hollinger

IPC分类号： G01N33/49 ,  B01F11/00 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G01N35/00 ,  G01N35/02

CPC分类号： B01F11/0014 ,  G01N2035/00524

摘要： Automatic biological sample mixing apparatus for use in flow cytometry, wherein a sample container is secured loosely at its top by means of a multi-reagent dispensing head; and the container is mounted at its bottom on a resilient support disposed on an elliptically rotated member, to cause reagents introduced into the container to be thoroughly mixed with a sample in the container in a fast, efficient, gentle, and accurately repeatable manner.

摘要翻译： 用于流式细胞术的自动生物样品混合装置，其中样品容器通过多试剂分配头松动地固定在其顶部; 并且容器在其底部安装在设置在椭圆转动构件上的弹性支撑件上，以使得引入到容器中的试剂以快速，有效，温和且准确的可重复的方式与容器中的样品充分混合。

公开(公告)号：US4395676A

公开(公告)日：1983-07-26

申请号：US209611

申请日：1980-11-24

申请人： John D. Hollinger ,  Michael R. Groves ,  Walter R. Hogg

发明人： John D. Hollinger ,  Michael R. Groves ,  Walter R. Hogg

IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N27/00

CPC分类号： G01N15/1218 ,  G01N2015/1037

摘要： A particle analyzer for a sample suspension of particles originating in a container in which: a flow director is mounted between the container and a first chamber, a particle sensing aperture is mounted between the first chamber and a second chamber, a liquid sheath is introduced into the first chamber for hydrodynamically focusing the particles through the sensing aperture, an electrical current passes through the sensing aperture for generating impedance signals, the pressure drop is regulated across the flow director to assure a constant flow of the suspension through the flow director and the aperture, and the flow director is formed into an optical element for viewing the sensing aperture.

摘要翻译： 一种用于来自容器的颗粒的样品悬浮液的颗粒分析仪，其中：导流器安装在容器和第一室之间，颗粒感测孔安装在第一室和第二室之间，将液体护套引入 用于通过感测孔流体动力地聚焦颗粒的第一腔室，电流通过感测孔径以产生阻抗信号，压力通过导流器被调节，以确保悬架通过导流器和孔径的恒定流动 并且导流器形成为用于观察感测孔的光学元件。