公开(公告)号：US20100154076A1

公开(公告)日：2010-06-17

申请号：US12600476

申请日：2008-05-26

申请人： Wesley B. Bruce ,  Xiping Niu

发明人： Wesley B. Bruce ,  Xiping Niu

IPC分类号： C12N15/82 ,  C07H21/00 ,  C12N15/74 ,  A01H5/00 ,  C07K14/00

CPC分类号： C07K14/415 ,  C12N15/8261 ,  Y02A40/146

摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include four ZmALF sequences. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1-8 as well as variants and fragments thereof. Nucleotide sequences encoding the maize alfins are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of one of four ZmALF sequences in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZmALF sequence of the invention. The level of the ZmALF polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括四种ZmALF序列。 本发明的组合物包含选自SEQ ID NO：1-8的氨基酸序列和核苷酸序列以及其变体和片段。 在DNA构建体中提供了编码玉米烯烃的核苷酸序列，用于在感兴趣的植物中表达以提供用于调节植物中的四种ZmALF序列之一或植物部分的水平。 所述方法包括向植物或植物部分引入包含本发明的ZmALF序列的异源多核苷酸。 可以增加或减少ZmALF多肽的水平。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

公开(公告)号：US20100186110A1

公开(公告)日：2010-07-22

申请号：US12602167

申请日：2008-06-01

申请人： Wesley B. Bruce ,  Shoba Sivasankar

发明人： Wesley B. Bruce ,  Shoba Sivasankar

IPC分类号： A01H1/00 ,  C12N5/10 ,  C12N15/82 ,  C07K14/415 ,  C07H21/04 ,  A01H5/00

CPC分类号： C12N15/8261 ,  Y02A40/146

摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a ZmRR10_p sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NO: 2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZmRR10_p are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZmRR10_p sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZmRR10_p sequence of the invention. The level of the ZmRR10_p polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括ZmRR10_p序列。 本发明的组合物包含选自SEQ ID NO：2的氨基酸序列和核苷酸序列以及其变体和片段。 在DNA构建体中提供编码ZmRR10_p的核苷酸序列，以提供用于在植物中表达的植物中的ZmRR10_p序列的水平或植物部分。 所述方法包括向植物或植物部分引入包含本发明的ZmRR10_p序列的异源多核苷酸。 可以增加或减少ZmRR10_p多肽的水平。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

公开(公告)号：US07422901B2

公开(公告)日：2008-09-09

申请号：US10987855

申请日：2004-11-12

申请人： Emil M. Orozco, Jr. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

发明人： Emil M. Orozco, Jr. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N15/87

CPC分类号： C07K14/415 ,  C12N15/8294

摘要： This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

摘要翻译： 本发明涉及编码生长素转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或大部分生长素转运蛋白的嵌合基因，其中嵌合基因的表达导致转化宿主中生长素转运蛋白水平的变化 细胞。 本发明还涉及使用生长素转运蛋白调节根发育的方法，以及发现具有潜在除草活性的化合物。

公开(公告)号：US20100175146A1

公开(公告)日：2010-07-08

申请号：US12602873

申请日：2008-06-06

申请人： Wesley B. Bruce ,  Olga N. Danilevskaya

发明人： Wesley B. Bruce ,  Olga N. Danilevskaya

IPC分类号： A01H1/00 ,  C12N15/00 ,  C12N15/29 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a ZMM28 sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1-2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZMM28 sequences are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZMM28 sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZMM28 sequence of the invention. The level of the ZMM28 polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括ZMM28序列。 本发明的组合物包含选自SEQ ID NO：1-2的氨基酸序列和核苷酸序列以及其变体和片段。 在DNA构建体中提供编码ZMM28序列的核苷酸序列，以提供用于在植物中表达的植物中的ZMM28序列的水平或植物部分。 所述方法包括向植物或植物部分引入包含本发明的ZMM28序列的异源多核苷酸。 可以增加或减少ZMM28多肽的水平。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

公开(公告)号：US07638681B2

公开(公告)日：2009-12-29

申请号：US12173868

申请日：2008-07-16

申请人： Emil M. Orozco, Jr. ,  Wesley B. Bruce ,  Rebecca E. Cahoon

发明人： Emil M. Orozco, Jr. ,  Wesley B. Bruce ,  Rebecca E. Cahoon

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C07K14/415 ,  C12N15/8294

摘要： This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

摘要翻译： 本发明涉及编码生长素转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或大部分生长素转运蛋白的嵌合基因，其中嵌合基因的表达导致转化宿主中生长素转运蛋白水平的变化 细胞。 本发明还涉及使用生长素转运蛋白调节根发育的方法，以及发现具有潜在除草活性的化合物。

公开(公告)号：US20090049574A1

公开(公告)日：2009-02-19

申请号：US12173868

申请日：2008-07-16

申请人： Emil M. Orozco, Jr. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

发明人： Emil M. Orozco, Jr. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8294

摘要： This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

摘要翻译： 本发明涉及编码生长素转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或大部分生长素转运蛋白的嵌合基因，其中嵌合基因的表达导致转化宿主中生长素转运蛋白水平的变化 细胞。 本发明还涉及使用生长素转运蛋白调节根发育的方法，以及发现具有潜在除草活性的化合物。

公开(公告)号：US06399859B1

公开(公告)日：2002-06-04

申请号：US08987367

申请日：1997-12-10

申请人： Scott E. Nichols ,  Kanwarpal S. Dhugga ,  George W. Singletary ,  Court A. Saunders ,  Derrick Witcher ,  Wesley B. Bruce ,  Lynne E. Sims ,  Guihua Lu ,  Gan-Yuan Zhong

发明人： Scott E. Nichols ,  Kanwarpal S. Dhugga ,  George W. Singletary ,  Court A. Saunders ,  Derrick Witcher ,  Wesley B. Bruce ,  Lynne E. Sims ,  Guihua Lu ,  Gan-Yuan Zhong

IPC分类号： C12N1582

CPC分类号： C12N9/0006 ,  A01K2217/05 ,  A23K10/30 ,  C12N15/8242 ,  C12N15/8245 ,  C12N15/8261 ,  C12N15/8286 ,  Y02A40/146 ,  Y02A40/162

摘要： Isolated nucleic acid molecules are provided that encode maize UDPGdH variant UDPGdH, and mutant UDPGdH proteins. These nucleic acid molecules can be used to produce transgenic plants having altered quality or quantity of starch. Also provided are vectors capable of expressing such nucleic acid molecules, host cells containing, such vectors, and polypeptides encoded by such nucleic acids.

摘要翻译： 提供了编码玉米UDPGdH变体UDPGdH和突变型UDPGdH蛋白质的分离的核酸分子。 这些核酸分子可用于产生具有改变的淀粉质量或数量的转基因植物。 还提供了能够表达这种核酸分子的载体，含有此类载体的宿主细胞和由该核酸编码的多肽。

公开(公告)号：US06388066B1

公开(公告)日：2002-05-14

申请号：US09405779

申请日：1999-09-27

申请人： Wesley B. Bruce ,  Sheila E. Maddock

发明人： Wesley B. Bruce ,  Sheila E. Maddock

IPC分类号： C07H2104

CPC分类号： C12N15/8216 ,  C12N15/822 ,  C12N15/8222 ,  C12N15/8227 ,  C12N15/8234

摘要： This invention discloses to compositions and methods for altering the characteristic pattern of expression associated with a promoter. More particularly the constitutive expression pattern associated with the small synthetic promoter, Rsyn7 is modified so that expression of a heterologous nucleotide sequence operably linked to the Rsyn7 promoter is in a tissue localized manner. This modification of the Rsyn7 pattern of expression occurs as a result of the addition of matrix attachment region DNA sequences to the flanks or 5′ and 3′ ends of an expression cassette comprising the Rsyn7 promoter operably linked to a heterologous nucleotide sequence of interest. DNA constructs, transformed plant cells and transformed plants are provided.

摘要翻译： 本发明公开了用于改变与启动子相关的表达特征模式的组合物和方法。 更具体地，与小合成启动子Rsyn7相关的组成型表达模式被修饰，使得可操作地连接到Rsyn7启动子的异源核苷酸序列的表达是组织定位的。 Rsyn7模式表达的这种修饰是由于将基质连接区DNA序列添加到包含可操作地连接到感兴趣的异源核苷酸序列的Rsyn7启动子的表达盒的侧翼或5'和3'末端而进行的。 提供了DNA构建体，转化的植物细胞和转化的植物。

公开(公告)号：US20100306874A1

公开(公告)日：2010-12-02

申请号：US12599515

申请日：2008-05-15

申请人： Wesley B. Bruce ,  Olga N. Danilevskaya ,  Lisa J. Newman

发明人： Wesley B. Bruce ,  Olga N. Danilevskaya ,  Lisa J. Newman

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  C12N9/12

CPC分类号： C12N15/8261 ,  C12N9/1205 ,  Y02A40/146

摘要： Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots, and for increasing yield in a plant are provided. The compositions include a ZmPKT sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZmPKT polypeptides are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZmPKT sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZmPKT sequence of the invention. The level of the ZmPKT polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

摘要翻译： 提供了用于调节花器官发育，叶形成，光向性，顶端优势，果实发育，根的起始和增加植物产量的组合物和方法。 组合物包括ZmPKT序列。 本发明的组合物包含选自SEQ ID NO：1和2的氨基酸序列和核苷酸序列以及其变体和片段。 在DNA构建体中提供了编码ZmPKT多肽的核苷酸序列，用于在感兴趣的植物中表达以提供植物中的ZmPKT序列的水平或植物部分。 所述方法包括向植物或植物部分引入包含本发明的ZmPKT序列的异源多核苷酸。 可以增加或减少ZmPKT多肽的水平。 这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

公开(公告)号：US07834240B2

公开(公告)日：2010-11-16

申请号：US12464144

申请日：2009-05-12

申请人： Mei Guo ,  Mary A. Rupe ,  Wesley B. Bruce ,  Rajeev Gupta ,  Carl R Simmons

发明人： Mei Guo ,  Mary A. Rupe ,  Wesley B. Bruce ,  Rajeev Gupta ,  Carl R Simmons

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  C07H21/04

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N15/8273 ,  C12Q1/6895 ,  Y02A40/146

摘要： The present invention provides polynucleotides and related polypeptides (SEQ ID NO: 3 and 4) of the ZmARGOS (Auxin-Regulated Gene involved in Organ Size) gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants. Transgenic plants expressing ZmARGOS show a positive impact on biomass accumulation and rate of maize plant growth, as well as an increase in organ size. These maize genes will find utility for enhancing agronomic traits in maize (and other crops).

摘要翻译： 本发明提供了ZmARGOS（涉及器官大小的生长素调节基因）基因家族的多核苷酸和相关多肽（SEQ ID NO：3和4）。 本发明提供了ZmARGOS基因的基因组序列。 ZmARGOS负责控制作物植物的生长，器官大小和产量。 表达ZmARGOS的转基因植物对生物量积累和玉米植物生长速率以及器官大小的增加有积极的影响。 这些玉米基因将用于提高玉米（和其他作物）的农艺性状。