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公开(公告)号：US20210040440A1
公开(公告)日：2021-02-11
申请号：US16967834
申请日：2019-02-06
申请人： White Dog Labs, Inc.
摘要： A selection method including providing a microorganism preparation including a variety of microorganisms and optionally water; providing an organic liquid including at least 70% by weight hydrophobic solvent; forming a multiple phase medium including a selected amount of the microorganism preparation and a selected amount of the organic liquid; and maintaining the multiple phase medium at a selected temperature for selected duration, whereby a treated microorganism preparation is formed.
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2.发明申请公开(公告)号：US20180305720A1
公开(公告)日：2018-10-25
申请号：US15765391
申请日：2016-09-30
申请人： White Dog Labs, Inc.
发明人： Bryan Patrick TRACY , Shawn William JONES , Daniel Knox MITCHELL , Aharon M. EYAL , Karthikeyan RAMACHANDRIYA
CPC分类号： C12P7/30 , B01D61/14 , B01D2311/25 , C12N9/1029 , C12P7/04 , C12P7/28 , C12P7/54 , C12P13/04 , Y02P20/582
摘要： Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.
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公开(公告)号：US20180355384A1
公开(公告)日：2018-12-13
申请号：US16002674
申请日：2018-06-07
申请人： White Dog Labs, Inc.
发明人： Bryan P. TRACY , Shawn William JONES , John Randall PHILLIPS , Daniel Knox MITCHELL , Aharon M. EYAL
摘要： An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.
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公开(公告)号：US20210030816A1
公开(公告)日：2021-02-04
申请号：US17067784
申请日：2020-10-12
申请人： WHITE DOG LABS, INC.
IPC分类号： A61K35/742 , A23K10/18 , A61K47/12 , A61K35/747 , A61K35/744
摘要： Provided is a feed ingredient comprising at least 20% by weight Clostridium tyrobutyricum, wherein said Clostridium tyrobutyricum comprises at least 70% of dry weight crude protein and optionally at least 7% of dry weight lysine and/or at least 1% of dry weight methionine. Further provided are animal feeds and probiotic compositions comprising the feed ingredient, methods for the production thereof and uses thereof.
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公开(公告)号：US20210024962A1
公开(公告)日：2021-01-28
申请号：US16306186
申请日：2017-05-31
申请人： White Dog Labs, Inc.
摘要： Supplemented mixotrophic method. A mixotrophic fermentation method is disclosed including providing a naturally acetogenic organism; providing a fermentation medium comprising a carbon source and a supplemented non-sugar reductant; and culturing the organism in the fermentation medium, where both the carbon source and the non-sugar reductant are metabolized and a fermentation broth is formed, which contains at least one carbon-containing bioproduct.
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6.发明申请公开(公告)号：US20200063091A1
公开(公告)日：2020-02-27
申请号：US16672548
申请日：2019-11-04
申请人： WHITE DOG LABS, INC.
发明人： Bryan P. TRACY , Shawn William JONES , John Randall PHILLIPS , Daniel Knox MITCHELL , Aharon M. EYAL
摘要： Single cell protein products and an integrated method for the production of ethanol and single cell protein. A single-cell protein product comprising on dry basis between 62% by weight and 75% by weight crude protein, at least 0.2% by weight butyric acid and at least three of, at least four of or all five of (i) between 5.5% by weight and 6.9% by weight lysine; (ii) between 2.1% by weight and 3.5% by weight methionine; (iii) between 3.1% by weight and 4.4% by weight threonine; (iv) between 2.0% by weight and 3.3% by weight tryptophan and (v) between 2.5% by weight and 4.5% by weight crude fat.
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公开(公告)号：US20190144908A1
公开(公告)日：2019-05-16
申请号：US16308267
申请日：2017-06-09
申请人： White Dog Labs, Inc.
摘要： High cell density anaerobic fermentation for protein expression. A method for producing a protein is described, including providing a modified Butyribacterium methylotrophicum (Bme) strain host; providing a fermentation medium including a carbon source and a nitrogen source; culturing the modified host in the fermentation medium under anaerobic conditions, where the carbon source is metabolized and a fermentation broth including at least one protein is formed and optionally separating the protein; where the Bme cell density in the fermentation broth is greater than 15 g/L.
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公开(公告)号：US20200345036A1
公开(公告)日：2020-11-05
申请号：US16961990
申请日：2019-01-04
申请人： WHITE DOG LABS, INC.
发明人： Alon KARPOL , Hadar GILARY , Shawn William JONES , Bryan P. TRACY , Biniam MARU , Aharon M. EYAL
IPC分类号： A23K10/18 , A23K50/75 , A23L33/135
摘要： Methods and compositions utilizing organism consisting of the strains within the order Clostridiales for improving health of animals. Disclosed methods comprise administering an effective dose of a composition comprising organism consisting of the strains within the order Clostridiales to an animal, including for example, a broiler or hen, thereby improving said animal's health. Also disclosed are compositions including organism consisting of the strains within the order Clostridiales such as Eubacterium aggregans, acetobacterium woodii, Blautia producta, Butyribacterium methylotrophicum, Clostridium acetobutylicum, Clostridium autoethanogenum, Clostridium beijerinckii, Clostridium butyricum, Clostridium carboxidivorans, Clostridium drakei, Clostridium ljungdahlii, Clostridium kluyveri, Clostridium pasteurianum, Clostridium saccharobutylicum, Clostridium saccharoperbutylacetonicum, Clostridium scatologenes, Clostridium tyrobutyricum, Eubacterium aggregans, Eubacterium limosum, Paraclostridium bifermentans, Oxobacter pfennigii, Sporomusa termitida, and Terrisporobacter glycolicus.use in such methods.
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9.发明申请公开(公告)号：US20190218578A1
公开(公告)日：2019-07-18
申请号：US15754937
申请日：2016-08-23
申请人： White Dog Labs, Inc.
CPC分类号： C12P7/28 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12P7/04 , C12P7/56 , C12Y101/01001 , C12Y102/0101 , C12Y102/01057 , C12Y102/07005 , C12Y203/01019 , C12Y207/02007
摘要： The present invention provides microorganisms capable of converting acetyl-coA into crotyl alcohol as well as fermentation methods for producing crotyl alcohol, either alone, or in combination with acetone and/or isopropanol. The microorganisms may be genetically engineered to express and/or disrupt one or more of the following enzymes: acetaldehyde dehydrogenase, alcohol dehydrogenase, bifunctional acetaldehyde/alcohol dehydrogenase, aldehyde oxidoreductase, phosphotransacetylase, acetate kinase, CoA-transferase A, CoA-transferase B, acetoacetate decarboxylase, secondary alcohol dehydrogenase, butyryl-CoA dehydro genase (BCD), and/or trans-2-enoyl-CoA reductase (TER).
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10.发明申请MIXOTROPHIC FERMENTATION METHOD FOR MAKING ACETONE, ISOPROPANOL, BUTYRIC ACID AND OTHER BIOPRODUCTS, AND MIXTURES THEREOF 有权制备丙酮，异丙酚，丁酸和其他生物产物的混合发酵方法及其混合物公开(公告)号：US20160251683A1
公开(公告)日：2016-09-01
申请号：US15055045
申请日：2016-02-26
申请人： White Dog Labs, Inc.
CPC分类号： C12P7/28 , C12P7/04 , C12P7/06 , C12P7/065 , C12P7/16 , C12P7/18 , C12P7/24 , C12P7/40 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： Mixotrophic fermentation method performed to make one or more bioproducts such as alcohols, organic acids of less than 7 carbons, acetone, 2,3-butanediol and mixtures thereof with a microorganism. The mixotrophic fermentation method includes providing an isolated organism and providing a first feedstock and a second feedstock for use in a fermentation medium. The first feedstock includes a carbon source that is metabolized by the native form of the organism at a rate of less than 0.01 g/hr/g cell mass, and the second feedstock includes CO, CO2, carbonate, bicarbonate, H2, glycerol, methanol, formate, urea or mixtures thereof. The mixotrophic fermentation method also includes culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, the broth having at least one bioproduct. Optionally, the bioproduct may be separated from the broth.
摘要翻译： 进行混合营养发酵方法以制备一种或多种生物产物如醇，少于7个碳的有机酸，丙酮，2,3-丁二醇及其与微生物的混合物。 混合发酵方法包括提供分离的生物并提供用于发酵培养基的第一原料和第二原料。 第一原料包括以小于0.01g / hr / g细胞质量的速率由天然形式的生物体代谢的碳源，第二原料包括CO，CO 2，碳酸盐，碳酸氢盐，H 2，甘油，甲醇 ，甲酸盐，尿素或其混合物。 混合发酵方法还包括在发酵培养基中培养生物体，由此两种原料被代谢并形成发酵液，所述肉汤具有至少一种生物产物。 任选地，生物产物可以与肉汤分离。
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