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11 条记录 (耗时 0.043 秒)

    FEEDING BUFFERS, SYSTEMS, AND METHODS FOR IN VITRO SYNTHESIS OF BIOMOLECULES
    1.
    发明申请
    FEEDING BUFFERS, SYSTEMS, AND METHODS FOR IN VITRO SYNTHESIS OF BIOMOLECULES 审中-公开
    饲料缓冲液，系统和方法用于生物分子的体外合成

    公开(公告)号：US20120190064A1

    公开(公告)日：2012-07-26

    申请号：US13356368

    申请日：2012-01-23

    申请人： Wieslaw Antoni KUDLICKI ,  Shiranthi Keppetipola ,  Julia Fletcher ,  Ashley Elaine Getbehead ,  Federico Katzen ,  Laura Vozza-Brown

    发明人： Wieslaw Antoni KUDLICKI ,  Shiranthi Keppetipola ,  Julia Fletcher ,  Ashley Elaine Getbehead ,  Federico Katzen ,  Laura Vozza-Brown

    IPC分类号： C12P21/02 ,  C12N9/12

    CPC分类号： C12P21/00 ,  C07K1/02 ,  C12N15/70 ,  C12P21/02

    摘要： Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

    摘要翻译： 本文提供了用于合成生物分子如多肽的体外系统的组合物，方法和试剂盒。 提供了使用体外蛋白质合成方法提供增加的可溶性蛋白质产量的细胞提取物。 本发明还包括通过添加包括氨基酸和进入体外合成系统的能量源的进料溶液来生产高产量蛋白质的方法。 本发明还包括使用高产量体外合成系统产生大量具有掺入标记氨基酸的蛋白质以通过诸如NMR的方法进行分析的方法。 本发明还包括使用体外合成系统从核酸模板增强蛋白质生产的载体。

    FEEDING BUFFERS, SYSTEMS, AND METHODS FOR IN VITRO SYNTHESIS OF BIOMOLECULES
    2.
    发明申请
    FEEDING BUFFERS, SYSTEMS, AND METHODS FOR IN VITRO SYNTHESIS OF BIOMOLECULES 审中-公开
    饲料缓冲液，系统和方法用于生物分子的体外合成

    公开(公告)号：US20090209032A1

    公开(公告)日：2009-08-20

    申请号：US12211094

    申请日：2008-09-15

    申请人： Wieslaw Antoni Kudlicki ,  Shiranthi Keppetipola ,  Julia Fletcher ,  Ashley Elaine Getbehead ,  Federico Katzen ,  Laura Vozza-Brown

    发明人： Wieslaw Antoni Kudlicki ,  Shiranthi Keppetipola ,  Julia Fletcher ,  Ashley Elaine Getbehead ,  Federico Katzen ,  Laura Vozza-Brown

    IPC分类号： C12N15/74 ,  C07K2/00

    CPC分类号： C12P21/00 ,  C07K1/02 ,  C12N15/70 ,  C12P21/02

    摘要： Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

    摘要翻译： 本文提供了用于合成生物分子如多肽的体外系统的组合物，方法和试剂盒。 提供了使用体外蛋白质合成方法提供增加的可溶性蛋白质产量的细胞提取物。 本发明还包括通过添加包括氨基酸和进入体外合成系统的能量源的进料溶液来生产高产量蛋白质的方法。 本发明还包括使用高产量体外合成系统产生大量具有掺入标记氨基酸的蛋白质以通过诸如NMR的方法进行分析的方法。 本发明还包括使用体外合成系统从核酸模板增强蛋白质生产的载体。

    Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
    3.
    发明申请
    Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 审中-公开
    饲养缓冲液，系统和生物分子体外合成方法

    公开(公告)号：US20060110788A1

    公开(公告)日：2006-05-25

    申请号：US11240651

    申请日：2005-10-03

    申请人： Wieslaw Kudlicki ,  Shiranthi Keppetipola ,  Julia Fletcher ,  Ashley Getbehead ,  Federico Katzen ,  Laura Vozza-Brown

    发明人： Wieslaw Kudlicki ,  Shiranthi Keppetipola ,  Julia Fletcher ,  Ashley Getbehead ,  Federico Katzen ,  Laura Vozza-Brown

    IPC分类号： C12P21/06 ,  C12N15/74

    CPC分类号： C12P21/00 ,  C07K1/02 ,  C12N15/70 ,  C12P21/02

    摘要： Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

    摘要翻译： 本文提供了用于合成生物分子如多肽的体外系统的组合物，方法和试剂盒。 提供了使用体外蛋白质合成方法提供增加的可溶性蛋白质产量的细胞提取物。 本发明还包括通过添加包括氨基酸和进入体外合成系统的能量源的进料溶液来生产高产量蛋白质的方法。 本发明还包括使用高产量体外合成系统产生大量具有掺入标记氨基酸的蛋白质以通过诸如NMR的方法进行分析的方法。 本发明还包括使用体外合成系统从核酸模板增强蛋白质生产的载体。

    Electroporation apparatus and methods
    10.
    发明申请
    Electroporation apparatus and methods 审中-公开
    电穿孔仪和方法

    公开(公告)号：US20060281182A1

    公开(公告)日：2006-12-14

    申请号：US11407910

    申请日：2006-04-21

    申请人： Laura Vozza-Brown ,  Harry Yim ,  John Cameron ,  Matthew O'Banion ,  Adam Henry ,  Lisa Olivier

    发明人： Laura Vozza-Brown ,  Harry Yim ,  John Cameron ,  Matthew O'Banion ,  Adam Henry ,  Lisa Olivier

    IPC分类号： C12N15/87

    CPC分类号： C12N15/87 ,  C12M23/08 ,  C12M35/02

    摘要： This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a gap between two electrode surfaces. Further embodiments of devices and methods described herein may eliminate the need for an end user to transfer competent cells from a capped tube to electroporation cuvette, thereby saving time and producing less waste.

    摘要翻译： 本文件公开了电穿孔容器，已经在电穿孔容器中等分并冷冻的电感受态细胞，以及用于电穿孔的许多其它装置，试剂盒和方法。 本文所述的电穿孔容器的一些实施例可以包括在两个电极表面之间的间隙中朝向彼此向下成角度的一对相对的壁。 本文描述的装置和方法的其它实施例可以消除对终端用户将有能力的细胞从封盖管转移到电穿孔试管的需要，从而节省时间并减少浪费。