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    Biocidal proteins
    1.
    发明授权
    Biocidal proteins 失效
    杀生物蛋白

    公开(公告)号：US06521590B1

    公开(公告)日：2003-02-18

    申请号：US09298574

    申请日：1999-04-29

    申请人： Willem Frans Broekaert ,  Bruno Philippe Angelo Cammue ,  Rupert William Osborn ,  Sarah Bronwen Rees ,  Jozef Vanderleyden

    发明人： Willem Frans Broekaert ,  Bruno Philippe Angelo Cammue ,  Rupert William Osborn ,  Sarah Bronwen Rees ,  Jozef Vanderleyden

    IPC分类号： A61K3808

    CPC分类号： C12N15/8281 ,  A01N65/20 ,  A01N65/32 ,  A01N65/38 ,  A01N65/44 ,  A61K38/00 ,  C07K14/415 ,  C12N15/8282

    摘要： Biocidal proteins capable of isolation from seeds have been characterized. The proteins have an amino acid sequence containing the common cysteine/glycine domain of Chitin-binding Plant Proteins but show substantially better activity against pathogenic fungi, a higher ratio of basic amino acids to acidic amino acids, and/or antifungal activity which results in increased hyphal branching. Antimicrobial proteins isolated from Amaranthus, Capsicum, Briza and related species are provided. The proteins show a wide range of antifungal activity and are active against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants may be transformed with this DNA. The proteins find agricultural or pharmaceutical application as antifungal or antibacterial agents. Transgenic plants expressing the protein will show increased disease resistance.

    摘要翻译： 已经表征了能够从种子中分离的杀生物蛋白质。 蛋白质具有含有几丁质结合植物蛋白质的共同半胱氨酸/甘氨酸结构域的氨基酸序列，但对病原真菌显示出更好的活性，碱性氨基酸与酸性氨基酸的比例更高，和/或抗真菌活性增加 菌丝分枝。 提供了从苋属，辣椒，Briza和相关物种分离的抗菌蛋白质。 蛋白质显示出广泛的抗真菌活性，并且对革兰氏阳性菌具有活性。 编码蛋白质的DNA可以被分离并并入载体中。 可以用这种DNA转化植物。 蛋白质发现农业或药物应用作为抗真菌剂或抗菌剂。 表达蛋白质的转基因植物将显示出增加的抗病性。

    DNA encoding biocidal proteins
    3.
    发明授权
    DNA encoding biocidal proteins 失效
    编码杀生物蛋白的DNA

    公开(公告)号：US5691199A

    公开(公告)日：1997-11-25

    申请号：US451566

    申请日：1995-05-26

    申请人： Willem Frans Broekaert ,  Bruno Philippe Angelo Cammue ,  Sarah Bronwen Rees ,  Jozef Vanderleyden

    发明人： Willem Frans Broekaert ,  Bruno Philippe Angelo Cammue ,  Sarah Bronwen Rees ,  Jozef Vanderleyden

    IPC分类号： A01N65/20 ,  A01N65/32 ,  A01N65/38 ,  A01N65/44 ,  A61K38/00 ,  C07K14/415 ,  C12N15/82 ,  C12N15/29

    CPC分类号： C12N15/8281 ,  A01N65/20 ,  A01N65/32 ,  A01N65/38 ,  A01N65/44 ,  C07K14/415 ,  C12N15/8282 ,  A61K38/00

    摘要： Biocidal proteins capable of isolation from seeds have been characterized. The proteins have an amino acid sequence containing the common cysteine/glycine domain of Chitin-binding Plant Proteins but show substantially better activity against pathogenic fungi, a higher ratio of basic amino acids to acidic amino acids, and/or antifungal activity which results in increased hyphal branching. Antimicrobial proteins isolated from Amaranthus, Capsicum, Briza and related species are provided. The proteins show a wide range of antifungal activity and are active against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants may be transformed with this DNA. The proteins find agricultural or pharmaceutical application as antifungal or antibacterial agents. Transgenic plants expressing the protein will show increased disease resistance.

    摘要翻译： 已经表征了能够从种子中分离的杀生物蛋白质。 蛋白质具有含有几丁质结合植物蛋白质的共同半胱氨酸/甘氨酸结构域的氨基酸序列，但对病原真菌显示出更好的活性，碱性氨基酸与酸性氨基酸的比例更高，和/或抗真菌活性增加 菌丝分枝。 提供了从苋属，辣椒，Briza和相关物种分离的抗菌蛋白质。 蛋白质显示出广泛的抗真菌活性，并且对革兰氏阳性菌具有活性。 编码蛋白质的DNA可以被分离并并入载体中。 可以用这种DNA转化植物。 蛋白质发现农业或药物应用作为抗真菌剂或抗菌剂。 表达蛋白质的转基因植物将显示出增加的抗病性。

    Enhanced Expression
    8.
    发明申请
    Enhanced Expression 审中-公开
    增强表达

    公开(公告)号：US20080092252A1

    公开(公告)日：2008-04-17

    申请号：US10581472

    申请日：2004-11-30

    申请人： Bruno Philippe Angelo Cammue ,  Miguel Francesco Coleta De Bolle ,  Katleen Butaye

    发明人： Bruno Philippe Angelo Cammue ,  Miguel Francesco Coleta De Bolle ,  Katleen Butaye

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/00 ,  C12P21/04

    CPC分类号： C12N15/822

    摘要： Disclosed herein are methods and means of achieving enhanced expression of a target nucleotide sequence in a transgenic organism, which methods comprise the steps of: (i) providing an organism in which post-transcriptional gene silencing (PTGS) is suppressed, (ii) associating said target nucleotide sequence with one or more heterologous Matrix Attachment Region (MARs), and (iii) causing or permitting expression from the target nucleotide sequence in the organism. Unexpectedly, the MARs do not merely relieve gene silencing, but can actually lead to expression levels higher than can be achieved in wild-type organisms and higher than expression levels in organisms in which PTGS is suppressed but where the MARs are not employed.

    摘要翻译： 本文公开了在转基因生物体中实现靶核苷酸序列增强表达的方法和方法，该方法包括以下步骤：（i）提供抑制转录后基因沉默（PTGS）的生物体，（ii）缔合 所述靶核苷酸序列具有一个或多个异源基质附着区（MAR），和（iii）引起或允许从生物体中的靶核苷酸序列表达。 意外的是，MAR不仅仅是缓解基因沉默，而且实际上可以导致在野生型生物体中可以达到的表达水平高于PTGS被抑制但没有使用MAR的生物中的表达水平。