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33 条记录 (耗时 0.029 秒)

    Thermochromic drawing device
    1.
    发明授权
    Thermochromic drawing device 失效
    热变色拉丝装置

    公开(公告)号：US5441418A

    公开(公告)日：1995-08-15

    申请号：US065549

    申请日：1993-05-20

    申请人： William E. Brown ,  Charles W. Dietterich ,  Richard M. Mandle

    发明人： William E. Brown ,  Charles W. Dietterich ,  Richard M. Mandle

    IPC分类号： B41M5/28 ,  G09B11/00

    CPC分类号： G09B11/00 ,  B41M5/282

    摘要： A thermochromic drawing device is disclosed which can be marked and erased by selectively changing the temperature of its drawing surface. The drawing surface of the drawing device consists of a flexible substrate printed with thermochromic inks having at least two color states. The flexible substrate has low thermal mass thereby enabling it to readily assimilate temperatures induced by a user and, consequently, facilitating the transition of the thermochromic inks from one color state to another. The drawing surface is further provided with an insulating layer which isolates the flexible substrate from unwanted heat sinks and heat sources thereby preventing unwanted color transitions of the thermochromic inks. Thus, a user can selectively change the color state of portions of the drawing surface to create distinctive markings.

    摘要翻译： 公开了一种通过选择性地改变其拉制表面的温度来标记和擦除的热致变色拉丝装置。 拉伸装置的拉伸表面由具有至少两种颜色状态的热变色油墨印刷的柔性基材组成。 柔性基板具有低热量，从而使其能够容易地吸收用户感应的温度，从而促进热变色油墨从一种颜色状态到另一种颜色状态的转变。 拉伸表面还设置有绝缘层，其将柔性基板与不需要的散热器和热源隔离，从而防止热变色油墨的不期望的颜色转变。 因此，用户可以选择性地改变绘图表面的部分的颜色状态以产生不同的标记。

    Enzyme amplification and purification
    2.
    发明授权
    Enzyme amplification and purification 失效

    公开(公告)号：US4767708A

    公开(公告)日：1988-08-30

    申请号：US638638

    申请日：1984-08-07

    申请人： Edwin G. Minkley, Jr. ,  William E. Brown

    发明人： Edwin G. Minkley, Jr. ,  William E. Brown

    IPC分类号： C12N9/12 ,  C12N1/20 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C12N9/1252

    摘要： Restriction enzymes are used to remove from DNA a complete and undamaged structural gene coding region for the expression of DNA polymerase I (polA) without the gene's natural promoter or with only a significantly damaged portion of the gene's natural promoter. Also by the use of restriction enzymes, a segment from a plasmid cloning vector is excised at a position adjacent to a promoter which is conditionally controllable and may be more powerful than the damaged or removed promoter. The gene for DNA polymerase I is enzymatically cloned into said vector at the position of said removed segment and adjacent to said conditionally controllable promoter. Multicopies of the cloned vector are introduced into a host baterial strain (E. coli). The host strain is then cultured so that the cell colony grows and replicates new generations containing replicated foreign plasmid. During such said replication the activity of said controllable promoter is repressed. After the cell colony has grown, the repression of said controllable promoter is removed and the cells express an amplified amount of DNA polymerase I which is lethal or inhibitory to the cells. An improved procedure is disclosed comprising a sequence of steps for harvesting purified DNA polymerase I.

    Method for isolating polyether antibiotics
    3.
    发明授权
    Method for isolating polyether antibiotics 失效
    分离聚醚抗生素的方法

    公开(公告)号：US4213966A

    公开(公告)日：1980-07-22

    申请号：US802768

    申请日：1977-06-02

    申请人： Wen-Chih Liu ,  William E. Brown ,  Gail L. Astle

    发明人： Wen-Chih Liu ,  William E. Brown ,  Gail L. Astle

    IPC分类号： C12P1/06 ,  C12P17/18 ,  A61K35/00 ,  C07H15/22

    CPC分类号： C12R1/465 ,  C12P1/06 ,  C12P17/181

    摘要： A method is provided for recovering a polyether antibiotic, such as lonomycin, monensin or ionomycin from a fermentation broth, wherein the polyether is first extracted with a water-immiscible solvent, such as ethyl acetate, and the resulting extracts concentrated, dissolved in aqueous methanol and then extracted into hexane, benzene or mixtures thereof. The polyether is thereby recovered in crystalline form directly from the latter extract.

    摘要翻译： 提供一种用于从发酵液中回收聚醚类抗生素如赖诺霉素，莫能菌素或离子霉素的方法，其中首先用与水不混溶的溶剂如乙酸乙酯萃取聚醚，所得提取物浓缩，溶于甲醇水溶液 然后萃取到己烷，苯或其混合物中。 因此，聚醚直接从后者的提取物以结晶形式回收。

    WEB-Based Task Management System and Method
    4.
    发明申请
    WEB-Based Task Management System and Method 审中-公开
    基于WEB的任务管理系统和方法

    公开(公告)号：US20080244582A1

    公开(公告)日：2008-10-02

    申请号：US12058605

    申请日：2008-03-28

    申请人： William E. Brown ,  Jason S. Astorquia ,  Todd W. Jones ,  Todd A. Fasullo ,  John D. Creason ,  Mark P. Mader ,  Maria R. Frei ,  Brent R. Frei

    发明人： William E. Brown ,  Jason S. Astorquia ,  Todd W. Jones ,  Todd A. Fasullo ,  John D. Creason ,  Mark P. Mader ,  Maria R. Frei ,  Brent R. Frei

    IPC分类号： G06F9/46

    CPC分类号： G06F16/955 ,  G06F9/54 ,  G06F16/211 ,  H04L12/1822 ,  H04L51/34 ,  H04L67/02

    摘要： A task management system and method integrates rich functionality into a web-browser based application. An efficient request for an update enables a user to quickly generate a completely customizable email message to intended recipient(s). By introducing a client side, in-memory database, the client component becomes less susceptible to network connectivity glitches and enables user interfaces to be redrawn without server interaction. Additionally, the task management system and method provides flexibility by enabling tasks to be grouped and organized. Specifically, a task may be associated with multiple task sheets and a task sheet may include multiple tasks in a many-to-many manner. Also, templates may be created that enable a user to start with a base template and to add (or remove) one or more columns. Further, the task management systems allows for multiple users to access and manipulate task data concurrently. In addition, the task management system provides a means for viewing the change history of task data within a task sheet by highlighting task data within a task sheet that has been changed by another user of the task management system.

    摘要翻译： 任务管理系统和方法将丰富的功能集成到基于Web浏览器的应用程序中。 对更新的有效请求使得用户能够快速地向预期收件人生成完全可定制的电子邮件消息。 通过引入客户端内存数据库，客户端组件变得不那么容易受到网络连接故障的影响，并且可以在不进行服务器交互的情况下重新绘制用户界面。 此外，任务管理系统和方法通过使任务分组和组织来提供灵活性。 特别地，任务可以与多个任务表相关联，并且任务表可以以多对多的方式包括多个任务。 此外，可以创建允许用户从基本模板开始并添加（或删除）一个或多个列的模板。 此外，任务管理系统允许多个用户同时访问和操纵任务数据。 此外，任务管理系统提供了通过突出显示已经由任务管理系统的另一用户改变的任务表内的任务数据来查看任务表内的任务数据的改变历史的手段。

    Purification method for materials having nick-translation ability
    5.
    发明授权
    Purification method for materials having nick-translation ability 失效
    具有切口翻译能力的材料的净化方法

    公开(公告)号：US5432072A

    公开(公告)日：1995-07-11

    申请号：US055832

    申请日：1993-04-30

    申请人： William E. Brown

    发明人： William E. Brown

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252 ,  Y10S435/815 ,  Y10S435/816

    摘要： The present invention pertains to a method of purification of DNA polymerase I, and the polymerase and Nick-translation activities thereof. In one embodiment, the method of purification is directed to circumstances where there are amplified amounts of the same relative to that which is found naturally occurring. In another embodiment, the purification method is directed to shortening the time period for the purification of the same whether in an amplified amount or not as compared to the time taught in the prior art.

    摘要翻译： 本发明涉及DNA聚合酶I的纯化方法及其聚合酶和尼克翻译活性。 在一个实施方案中，纯化方法涉及相对于天然存在的扩增量相同的情况。 在另一个实施方案中，与现有技术中教导的时间相比，纯化方法旨在缩短其纯化的时间段，无论放大量是否为放大量。

    Method of determining the open circuit voltage of a battery in a closed circuit
    6.
    发明授权
    Method of determining the open circuit voltage of a battery in a closed circuit 失效
    确定闭路电池的开路电压的方法

    公开(公告)号：US4204153A

    公开(公告)日：1980-05-20

    申请号：US939157

    申请日：1978-09-01

    申请人： William E. Brown

    发明人： William E. Brown

    IPC分类号： G01R31/36 ,  G01N27/42

    CPC分类号： G01R19/16542 ,  G01R31/3662

    摘要： The open circuit voltage of a battery which is connected in a closed circuit is determined without breaking the circuit or causing voltage upsets therein. The closed circuit voltage across the battery and the current flowing through it are determined under normal load and then a fractional change is made in the load and the new current and voltage values determined. The open circuit voltage is then calculated, according to known principles, from the two sets of values.

    摘要翻译： 在闭合电路中连接的电池的开路电压被确定，而不会断开电路或引起其中的电压扰动。 在正常负载下确定电池两端的闭路电压和流经电流的电流，然后在负载中进行分数变化，并确定新的电流和电压值。 然后根据已知原理从两组值计算开路电压。