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    POROUS MEMBRANE DEVICE THAT PROMOTES THE DIFFERENTIATION OF MONOCYTES INTO DENDRITIC CELLS
    3.
    发明申请
    POROUS MEMBRANE DEVICE THAT PROMOTES THE DIFFERENTIATION OF MONOCYTES INTO DENDRITIC CELLS 审中-公开
    促进单核细胞分化成细胞的多孔膜装置

    公开(公告)号：US20100105135A1

    公开(公告)日：2010-04-29

    申请号：US12609060

    申请日：2009-10-30

    申请人： Donald DRAKE, III ,  David MOE ,  Conan LI ,  Heather FAHLENKAMP ,  Guzman SANCHEZ-SCHMITZ ,  Russell HIGBEE ,  Robert PARKHILL ,  William L. WARREN

    发明人： Donald DRAKE, III ,  David MOE ,  Conan LI ,  Heather FAHLENKAMP ,  Guzman SANCHEZ-SCHMITZ ,  Russell HIGBEE ,  Robert PARKHILL ,  William L. WARREN

    IPC分类号： C12N5/0784

    CPC分类号： C12N5/0639 ,  C12M23/12 ,  C12M23/20 ,  C12M25/02 ,  C12M25/14 ,  C12N2502/28 ,  C12N2503/00

    摘要： Dendritic cells (DCs) for research and clinical applications are typically derived from purified blood monocytes that are cultured in a cocktail of cytokines for a week or more. Because it has been suggested that these cytokine-derived DCs may be deficient in some important immunological functions and might not accurately represent antigen-presenting cell (APC) populations found under physiologic conditions, there is a need for methods that allow the generation of DCs in a more physiologically relevant manner. The present invention comprises a simple and reliable technique for generating large numbers of highly purified DCs, based on a single migration of blood monocytes through endothelial cells that are cultured in, for example, a Transwell® device. The resultant APCs, harvested from the lower Transwell® chamber, resemble other in vitro-generated DC populations in their expression of major histocompatibility (MHC) and costimulatory molecules, ability to phagocytose foreign antigens, and capacity to trigger antigen-specific T cell responses.

    摘要翻译： 用于研究和临床应用的树突状细胞（DC）通常衍生自在细胞因子混合物中培养一周或更长时间的纯化血液单核细胞。 由于已经提出这些细胞因子衍生的DC可能在一些重要的免疫功能上有缺陷，并且可能不能准确地代表在生理条件下发现的抗原呈递细胞（APC）群体，所以需要一种允许在 更生理相关的方式。 本发明包括一种用于产生大量高度纯化的DC的简单且可靠的技术，其基于通过在例如Transwell装置中培养的内皮细胞的血液单核细胞的单次迁移。 从下部Transwell室获取的所得APC类似于其主要组织相容性（MHC）和共刺激分子的表达的其他体外产生的DC群体，吞噬外来抗原的能力和触发抗原特异性T细胞应答的能力。

    ARTIFICIAL TISSUE CONSTRUCTS COMPRISING ALVEOLAR CELLS AND METHODS FOR USING THE SAME
    8.
    发明申请
    ARTIFICIAL TISSUE CONSTRUCTS COMPRISING ALVEOLAR CELLS AND METHODS FOR USING THE SAME 有权
    包含神经细胞的人造组织构造及其使用方法

    公开(公告)号：US20090075282A1

    公开(公告)日：2009-03-19

    申请号：US12173921

    申请日：2008-07-16

    申请人： Ayesha MAHMOOD ,  Anatoly KACHURIN ,  William WARREN ,  Russell HIGBEE

    发明人： Ayesha MAHMOOD ,  Anatoly KACHURIN ,  William WARREN ,  Russell HIGBEE

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12Q1/02

    CPC分类号： A61K9/70 ,  A61K35/17 ,  A61K35/36 ,  C12N5/0688 ,  C12N2502/28 ,  C12N2533/54 ,  G01N33/5082 ,  G01N2800/12

    摘要： The present invention comprises artificial tissue constructs that serve as in vitro models of mammalian lung tissue. The artificial tissue constructs of the present invention comprise functionally equivalent in vitro tissue scaffolds that enable immunophysiological function of the lung. The constructs can serve as novel platforms for the study of lung diseases (e.g., interstitial lung diseases, fibrosis, influenza, RSV) as well as smoke- and smoking-related diseases. The artificial tissue constructs of the present invention comprise the two components of alveolar tissue, epithelial and endothelial cell layers.

    摘要翻译： 本发明包括用作哺乳动物肺组织的体外模型的人造组织构建体。 本发明的人造组织构建体包括能够实现肺的免疫生理功能的功能上等同的体外组织支架。 这些构建体可以作为研究肺部疾病（例如间质性肺疾病，纤维化，流感，RSV）以及吸烟和吸烟相关疾病的新平台。 本发明的人造组织构建体包含肺泡组织，上皮和内皮细胞层的两个组分。