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公开(公告)号：US5500356A
公开(公告)日：1996-03-19
申请号：US103769
申请日：1993-08-10
申请人： Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
发明人： Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
CPC分类号： C12Q1/6809 , C12N15/1013 , C12N15/1048 , C12Q1/6811 , C12Q1/6813 , C12Q1/6848
摘要： The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.
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公开(公告)号：US5759778A
公开(公告)日：1998-06-02
申请号：US525140
申请日：1995-09-08
申请人： Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
发明人： Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
CPC分类号： C12Q1/6809 , C12N15/1013 , C12N15/1048 , C12Q1/6811 , C12Q1/6813 , C12Q1/6848
摘要： The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.
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公开(公告)号：US20080287321A1
公开(公告)日：2008-11-20
申请号：US11480575
申请日：2006-07-05
申请人： Wu Bo Li , Joel A. Jessee , Christian E. Gruber
发明人： Wu Bo Li , Joel A. Jessee , Christian E. Gruber
IPC分类号： C40B50/06
CPC分类号： C12P19/34 , C07K16/40 , C12N15/1096
摘要： The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
摘要翻译： 本发明一般涉及制备cDNA分子和cDNA文库的方法。 本发明还涉及根据这些方法产生的cDNA分子和cDNA文库，以及含有此类cDNA分子和文库的载体和宿主细胞。 本发明还涉及用于制备本发明的cDNA分子和文库的试剂盒。
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公开(公告)号：US20100317063A1
公开(公告)日：2010-12-16
申请号：US12623375
申请日：2009-11-21
申请人： Wu Bo LI , Joel A. Jessee , Christian E. Gruber
发明人： Wu Bo LI , Joel A. Jessee , Christian E. Gruber
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C07K16/40 , C12N15/1096
摘要： The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
摘要翻译： 本发明一般涉及制备cDNA分子和cDNA文库的方法。 本发明还涉及根据这些方法产生的cDNA分子和cDNA文库，以及含有此类cDNA分子和文库的载体和宿主细胞。 本发明还涉及用于制备本发明的cDNA分子和文库的试剂盒。
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公开(公告)号：US07074556B2
公开(公告)日：2006-07-11
申请号：US09515513
申请日：2000-02-29
申请人： Wu Bo Li , Joel A. Jessee , Christian E. Gruber
发明人： Wu Bo Li , Joel A. Jessee , Christian E. Gruber
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C07K16/40 , C12N15/1096
摘要： The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
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公开(公告)号：US06589737B1
公开(公告)日：2003-07-08
申请号：US09574111
申请日：2000-05-19
申请人： Christian E. Gruber , Po-Jen Shih
发明人： Christian E. Gruber , Po-Jen Shih
IPC分类号： C12Q168
CPC分类号： C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
摘要： The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
摘要翻译： 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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公开(公告)号：US20080227107A1
公开(公告)日：2008-09-18
申请号：US12111834
申请日：2008-04-29
申请人： Christian E. Gruber , Po-Jen Shih
发明人： Christian E. Gruber , Po-Jen Shih
CPC分类号： C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
摘要： The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
摘要翻译： 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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公开(公告)号：US07037661B2
公开(公告)日：2006-05-02
申请号：US10405608
申请日：2003-04-03
申请人： Christian E. Gruber , Po-Jen Shih
发明人： Christian E. Gruber , Po-Jen Shih
CPC分类号： C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
摘要： The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
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公开(公告)号：US20100168408A1
公开(公告)日：2010-07-01
申请号：US12649662
申请日：2009-12-30
申请人： Christian E. Gruber , Po-Jen Shih
发明人： Christian E. Gruber , Po-Jen Shih
CPC分类号： C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
摘要： The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for to fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
摘要翻译： 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体而言，本发明涉及用于在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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公开(公告)号：US20090176242A1
公开(公告)日：2009-07-09
申请号：US12405127
申请日：2009-03-16
申请人： Christian E. Gruber , Po-Jen Shih
发明人： Christian E. Gruber , Po-Jen Shih
CPC分类号： C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
摘要： The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
摘要翻译： 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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