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公开(公告)号：US20100317063A1
公开(公告)日：2010-12-16
申请号：US12623375
申请日：2009-11-21
Applicant: Wu Bo LI , Joel A. Jessee , Christian E. Gruber
Inventor： Wu Bo LI , Joel A. Jessee , Christian E. Gruber
IPC: C12P19/34
CPC classification number: C12P19/34 , C07K16/40 , C12N15/1096
Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
Abstract translation: 本发明一般涉及制备cDNA分子和cDNA文库的方法。 本发明还涉及根据这些方法产生的cDNA分子和cDNA文库，以及含有此类cDNA分子和文库的载体和宿主细胞。 本发明还涉及用于制备本发明的cDNA分子和文库的试剂盒。
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公开(公告)号：US07074556B2
公开(公告)日：2006-07-11
申请号：US09515513
申请日：2000-02-29
Applicant: Wu Bo Li , Joel A. Jessee , Christian E. Gruber
Inventor： Wu Bo Li , Joel A. Jessee , Christian E. Gruber
IPC: C12Q1/68
CPC classification number: C12P19/34 , C07K16/40 , C12N15/1096
Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
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公开(公告)号：US5759778A
公开(公告)日：1998-06-02
申请号：US525140
申请日：1995-09-08
Applicant: Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
Inventor： Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
CPC classification number: C12Q1/6809 , C12N15/1013 , C12N15/1048 , C12Q1/6811 , C12Q1/6813 , C12Q1/6848
Abstract: The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.
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公开(公告)号：US20100168408A1
公开(公告)日：2010-07-01
申请号：US12649662
申请日：2009-12-30
Applicant: Christian E. Gruber , Po-Jen Shih
Inventor： Christian E. Gruber , Po-Jen Shih
CPC classification number: C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
Abstract: The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for to fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
Abstract translation: 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体而言，本发明涉及用于在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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公开(公告)号：US20090176242A1
公开(公告)日：2009-07-09
申请号：US12405127
申请日：2009-03-16
Applicant: Christian E. Gruber , Po-Jen Shih
Inventor： Christian E. Gruber , Po-Jen Shih
CPC classification number: C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
Abstract: The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
Abstract translation: 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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Patent Agency Ranking
公开(公告)号：US5500356A
公开(公告)日：1996-03-19
申请号：US103769
申请日：1993-08-10
Applicant: Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
Inventor： Wu-Bo Li , Christian E. Gruber , Joel A. Jessee , Jhy-Jhu Lin
CPC classification number: C12Q1/6809 , C12N15/1013 , C12N15/1048 , C12Q1/6811 , C12Q1/6813 , C12Q1/6848
Abstract: The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.
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公开(公告)号：US20120107879A1
公开(公告)日：2012-05-03
申请号：US13220433
申请日：2011-08-29
Applicant: Christian E. GRUBER , Joel A. Jessee
Inventor： Christian E. GRUBER , Joel A. Jessee
CPC classification number: C12N15/1003 , C12N15/101 , C12N15/1096
Abstract: The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.
Abstract translation: 本发明涉及生产和分离核酸分子的方法。 特别地，本发明涉及mRNA分子的分离以及可以是单链或双链的核酸分子（例如cDNA分子或文库）的产生和分离。 另外，本发明涉及从可能含有一群分子的样品中选择和分离特定的目标核酸分子。 具体地，本发明涉及亲和标记的引物 - 衔接子分子，其允许改进这种核酸分子的分离和产生，增加产物回收率和分离速度。
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公开(公告)号：US20080287321A1
公开(公告)日：2008-11-20
申请号：US11480575
申请日：2006-07-05
Applicant: Wu Bo Li , Joel A. Jessee , Christian E. Gruber
Inventor： Wu Bo Li , Joel A. Jessee , Christian E. Gruber
IPC: C40B50/06
CPC classification number: C12P19/34 , C07K16/40 , C12N15/1096
Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
Abstract translation: 本发明一般涉及制备cDNA分子和cDNA文库的方法。 本发明还涉及根据这些方法产生的cDNA分子和cDNA文库，以及含有此类cDNA分子和文库的载体和宿主细胞。 本发明还涉及用于制备本发明的cDNA分子和文库的试剂盒。
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公开(公告)号：US06589737B1
公开(公告)日：2003-07-08
申请号：US09574111
申请日：2000-05-19
Applicant: Christian E. Gruber , Po-Jen Shih
Inventor： Christian E. Gruber , Po-Jen Shih
IPC: C12Q168
CPC classification number: C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6844 , C12Q1/6862 , G01N27/44726 , C12Q2563/107 , C12Q2521/107 , C12Q2527/149
Abstract: The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
Abstract translation: 本发明通常涉及使用逆转录酶，优选多亚基逆转录酶（例如ASLV逆转录酶）来标记核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及在核酸合成期间荧光标记核酸分子的方法，试剂盒和组合物。 根据本发明产生的标记的核酸分子特别适用于用于核酸检测和诊断的标记探针。
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公开(公告)号：US20110151521A1
公开(公告)日：2011-06-23
申请号：US13018419
申请日：2011-01-31
Applicant: Wu Bo Li , Joel A. Jessee , Christian E. GRUBER
Inventor： Wu Bo Li , Joel A. Jessee , Christian E. GRUBER
CPC classification number: C12P19/34 , C07K16/40 , C12N15/1096
Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
Abstract translation: 本发明一般涉及制备cDNA分子和cDNA文库的方法。 本发明还涉及根据这些方法产生的cDNA分子和cDNA文库，以及含有此类cDNA分子和文库的载体和宿主细胞。 本发明还涉及用于制备本发明的cDNA分子和文库的试剂盒。
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