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公开(公告)号:US07094347B2
公开(公告)日:2006-08-22
申请号:US11035950
申请日:2005-01-18
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
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公开(公告)号:US07223341B2
公开(公告)日:2007-05-29
申请号:US11480977
申请日:2006-07-06
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译: 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜,其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜,其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用,包括离子交换层析,大分子转移,以及生物分子如蛋白质,核酸,内毒素等的检测,过滤和纯化。
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公开(公告)号:US06851561B2
公开(公告)日:2005-02-08
申请号:US10891098
申请日:2004-07-15
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
IPC分类号: B01D15/36 , B01D61/00 , B01D61/14 , B01D63/14 , B01D67/00 , B01D69/02 , B01D69/10 , B01D69/12 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01J20/28 , B01J41/12 , B01J41/20 , B01J47/12 , B01D63/06 , B01D71/56
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
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公开(公告)号:US07396465B2
公开(公告)日:2008-07-08
申请号:US11742749
申请日:2007-05-01
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译: 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜,其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜,其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用,包括离子交换层析,大分子转移,以及生物分子如蛋白质,核酸,内毒素等的检测,过滤和纯化。
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公开(公告)号:US20050211621A1
公开(公告)日:2005-09-29
申请号:US11035950
申请日:2005-01-18
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
IPC分类号: B01D15/36 , B01D61/00 , B01D61/14 , B01D63/14 , B01D67/00 , B01D69/02 , B01D69/10 , B01D69/12 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01J20/28 , B01J41/12 , B01J41/20 , B01J47/12
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译: 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜,其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜,其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用,包括离子交换层析,大分子转移,以及生物分子如蛋白质,核酸,内毒素等的检测,过滤和纯化。
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公开(公告)号:US20060249445A1
公开(公告)日:2006-11-09
申请号:US11480977
申请日:2006-07-06
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
IPC分类号: B01D71/06
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译: 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜,其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜,其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用,包括离子交换层析,大分子转移,以及生物分子如蛋白质,核酸,内毒素等的检测,过滤和纯化。
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公开(公告)号:US06780327B1
公开(公告)日:2004-08-24
申请号:US09914163
申请日:2001-12-12
申请人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人: Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
IPC分类号: B01D6114
CPC分类号: B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要: The present invention provides a positively charged microporous membrane having a protein binding capacity about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译: 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微孔膜,其包含亲水性多孔基质和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜,其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用,包括离子交换层析,大分子转移,以及生物分子如蛋白质,核酸,内毒素等的检测,过滤和纯化。
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公开(公告)号:US5258478A
公开(公告)日:1993-11-02
申请号:US874748
申请日:1992-04-27
IPC分类号: C07D311/30 , C08F12/00 , C08F12/32 , C08F20/26 , C08F212/14 , C08F220/28 , C08L25/18 , C08F216/36 , C08F212/08 , C08F220/14 , C08F220/42
CPC分类号: C08L25/18 , C07D311/30 , C08F12/32
摘要: Polymers containing covalently-bonded, low self-absorbing, scintillating chromophores, polymerizable chromophores of the formula: ##STR1## wherein R.sub.1 is vinyl, .alpha.-methyl vinyl, vinyl phenyl, or vinyl benzyl, and R.sub.2 and R.sub.3 are independently hydrogen, alkyl, aryl, cyano, nitro, halo, or ether, and a process for the preparation of polymers containing low self-absorbing, scintillating chromophores.
摘要翻译: 包含共价键合的低自吸收闪烁发色团的聚合物,下式的可聚合发色团:其中R1是乙烯基,α-甲基乙烯基,乙烯基苯基或乙烯基苄基,R2和R3独立地是氢,烷基, 芳基,氰基,硝基,卤素或醚,以及制备含有低自吸收,闪烁发色团的聚合物的方法。
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9.
公开(公告)号:US5552551A
公开(公告)日:1996-09-03
申请号:US96956
申请日:1995-07-26
IPC分类号: C07D311/30 , C08F12/00 , C08F12/32 , C08F20/26 , C08F212/14 , C08F220/28 , C08L25/18 , C07D215/20 , C07D311/22 , C07D311/76
CPC分类号: C08L25/18 , C07D311/30 , C08F12/32
摘要: Proton-transfer, low self-absorbing chromophores of the formula: ##STR1## wherein R.sub.2 and R.sub.3 are independently hydrogen, alkyl, aryl, cyano, nitro, halo or an ether group, R.sub.4 is O or N--H, and R.sub.5 is thienyl, naphthyl, furanyl, pyrrolyl, phenyl vinyl, diphenyl vinyl, phenyl ethynyl, hydroxy chromonyl phenyl, didecyloxy hydroxy chromonyl phenyl, phenyl or ##STR2## wherein R.sub.1 is vinyl, vinyl phenyl, vinyl benzyl, alkyl ethenyl, or alkyl phenyl ethenyl, provided that when R.sub.5 is phenyl and R.sub.2 and R.sub.3 are hydrogen, R.sub.4 is N--H.
摘要翻译: 质子转移,低自吸发色团,其分子式如下:其中R2和R3独立地是氢,烷基,芳基,氰基,硝基,卤素或醚基,R4是O或NH,R5是噻吩基,萘基 ,呋喃基,吡咯基,苯基乙烯基,二苯基乙烯基,苯基乙炔基,羟基苯并二氢吡喃基苯基,二苯氧基羟基苯并吡喃基苯基或其中R1是乙烯基,乙烯基苯基,乙烯基苄基,烷基乙烯基或烷基苯基乙烯基, R5是苯基,R2和R3是氢,R4是NH。
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