公开(公告)号：US10100322B2

公开(公告)日：2018-10-16

申请号：US14895059

申请日：2014-06-02

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Maxim Itkin

IPC: C12N15/82

Abstract: The present invention relates to genetically modified plants by key genes involved in the biosynthesis of steroidal alkaloids. These plants have altered content of steroidal (glyco)alkaloids. Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids are provided.

公开(公告)号：US11957102B2

公开(公告)日：2024-04-16

申请号：US17859053

申请日：2022-07-07

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Prashant Sonawane ,  Maxim Itkin

IPC: A01H6/82 ,  C12N9/10 ,  C12N15/82

CPC classification number: A01H6/82 ,  C12N9/1059 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8247

Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.

公开(公告)号：US12232496B2

公开(公告)日：2025-02-25

申请号：US17040227

申请日：2019-03-21

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD

Inventor： Asaph Aharoni ,  Elisa Korenblum

IPC: A01N63/20 ,  A01G29/00 ,  A01N63/38 ,  A61K36/185 ,  A61K36/81 ,  C07D305/14 ,  C07J17/00

Abstract: Provided is a plant exudate, methods for obtaining a plant exudate by inducing the plant to secrete an exudate and systems for the collection of a plant exudate which include: one or more plant container including at least two discrete compartments each configured to accommodate a split root of a plant, the compartments being a root stimulating compartment including one or more input being in fluid communication with at least a source of a plant root stimulant, and a root exudate harvesting compartment, and a root exudate collection compartment in fluid communication with the root exudate harvesting compartment.

公开(公告)号：US20190048330A1

公开(公告)日：2019-02-14

申请号：US16108402

申请日：2018-08-22

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Samuel Bocobza ,  Tal Dahan ,  Avraham A. Levy

IPC: C12N9/22 ,  C12N15/62 ,  C12N15/113 ,  C07K14/08 ,  C12N15/82

Abstract: Disclosed herein are methods for gene targeting in a plant cell, as well as recombinant nucleic acid molecules used in these methods. Further disclosed are recombinant nucleic acid molecules comprising a target gene operably linked to a regulatory region of the UBQ10 gene.

公开(公告)号：US12129475B2

公开(公告)日：2024-10-29

申请号：US16484200

申请日：2018-02-08

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD. ,  Yissum Research Development Company of The Hebrew University of Jerusalem Ltd.

Inventor： Asaph Aharoni ,  Prashant Sonawane ,  Pablo D. Cardenas ,  Burdman Saul

IPC: C12N15/82 ,  C12N9/02 ,  C12N9/04

CPC classification number: C12N15/8279 ,  C12N9/0006 ,  C12N9/0071 ,  C12N15/8218 ,  C12N15/8243

Abstract: Disclosed herein are genetically modified plants having altered biological activity of 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plants have an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Further disclosed herein are genetically modified plants having altered expression of a gene encoding a 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or a 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plant has an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Methods of producing these genetically modified plants are also disclosed.

公开(公告)号：US12041907B2

公开(公告)日：2024-07-23

申请号：US17272685

申请日：2019-09-05

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Prashant Sonawane ,  Maxim Itkin ,  Adam Jozwiak

IPC: A01H6/82 ,  C12N9/10 ,  C12N15/82

CPC classification number: A01H6/82 ,  C12N9/1059 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8247

Abstract: Provided herein are genetically modified cells and genetically modified plants having increased or decreased expression of a cellulose synthase like G (CSLG) enzyme. These cells and plants may have an increased or decreased content a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin, compared to a corresponding unmodified cell or plant. Also provided herein are methods of producing a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a genetically modified cell, as well as methods of reducing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part, and methods of increasing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part.

公开(公告)号：US11412700B2

公开(公告)日：2022-08-16

申请号：US17008875

申请日：2020-09-01

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Prashant Sonawane ,  Maxim Itkin

IPC: A01H6/82 ,  C12N15/82 ,  C12N9/10

Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.

公开(公告)号：US10767201B2

公开(公告)日：2020-09-08

申请号：US15758372

申请日：2016-09-11

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Guy Polturak

IPC: C12P23/00 ,  C12P13/22 ,  C12P13/04 ,  A23L3/3463 ,  A23L3/3526 ,  A23L5/46 ,  A23L3/3544 ,  C07K14/415 ,  C12N9/02 ,  C12N9/12 ,  C12P17/12 ,  C12P19/02

Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4.5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.

公开(公告)号：US11932887B2

公开(公告)日：2024-03-19

申请号：US16940666

申请日：2020-07-28

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Guy Polturak

IPC: C12P13/22 ,  A23L3/3463 ,  A23L3/3526 ,  A23L3/3544 ,  A23L5/46 ,  C07K14/415 ,  C12N9/02 ,  C12N9/12 ,  C12P13/04 ,  C12P17/12 ,  C12P19/02

CPC classification number: C12P13/225 ,  A23L3/3463 ,  A23L3/3526 ,  A23L3/3544 ,  A23L5/46 ,  C07K14/415 ,  C12N9/0083 ,  C12N9/1205 ,  C12P13/04 ,  C12P17/12 ,  C12P19/02 ,  C12Y207/01095 ,  A23V2002/00 ,  A23V2002/00 ,  A23V2200/044

Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as Mirabilis. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.

公开(公告)号：US11104910B2

公开(公告)日：2021-08-31

申请号：US16108402

申请日：2018-08-22

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph Aharoni ,  Samuel Bocobza ,  Tal Dahan ,  Avraham A. Levy

IPC: C12N15/82 ,  C12N15/86 ,  C07K14/08

Abstract: Disclosed herein are methods for gene targeting in a plant cell, as well as recombinant nucleic acid molecules used in these methods. Further disclosed are recombinant nucleic acid molecules comprising a target gene operably linked to a regulatory region of the UBQ10 gene.