公开(公告)号：US20080145838A1

公开(公告)日：2008-06-19

申请号：US11818695

申请日：2007-06-15

申请人： Yasuo Suda ,  Tomoaki Nishimura ,  Yuko Kishimoto ,  Sakiko Yamashita ,  Sachiko Tsuruta ,  Masahiro Wakao ,  Tashiomi Okuno

发明人： Yasuo Suda ,  Tomoaki Nishimura ,  Yuko Kishimoto ,  Sakiko Yamashita ,  Sachiko Tsuruta ,  Masahiro Wakao ,  Tashiomi Okuno

IPC分类号： C12Q1/70 ,  C12Q1/02 ,  G01N33/00 ,  G01N33/48 ,  G01N33/92

CPC分类号： G01N33/569 ,  G01N33/5308 ,  G01N33/5432 ,  G01N33/68 ,  G01N33/92 ,  G01N2400/00

摘要： This invention provides a method of screening or patterning a biomaterial in terms of their specificities to sugar chains by performing real-time and comprehensive measurement of an interaction between sugar chains and the biomaterial concurrently with a very small amount of the biomaterial without labeling. It is a method of measuring an interaction between a biomaterial and a sugar chain(s), the method including: bringing a solution containing the biomaterial in contact with a ligand carrier, the ligand carrier including a support whose surface includes a metal, and a ligand conjugate(s) immobilized independently on the surface, the ligand carrier carrying the ligand conjugate(s) in such a manner that 1 to 500 kinds of the ligand conjugate(s) is immobilized per cm2, and each ligand conjugate having a structure in which a sugar chain is bonded with a linker compound having a sulfur atom, and the biomaterial being at least one selected from the groups of proteins, viruses, cells, microorganisms, liposome, and micelles.

摘要翻译： 本发明提供了一种通过与非标量的生物材料同时进行糖链与生物材料之间的相互作用的实时和全面测量来对其对糖链的特异性筛选或图案化生物材料的方法。 它是测量生物材料和糖链之间的相互作用的方法，该方法包括：使含有生物材料的溶液与配体载体接触，所述配体载体包括其表面包括金属的载体，和 配体缀合物独立地固定在表面上，携带配体缀合物的配体载体以每cm 2固定1至500种配体缀合物的方式， 并且每个配体缀合物具有其中糖链与具有硫原子的接头化合物结合的结构，并且所述生物材料是选自蛋白质，病毒，细胞，微生物，脂质体和胶束中的至少一种。

公开(公告)号：US09239329B2

公开(公告)日：2016-01-19

申请号：US11818695

申请日：2007-06-15

申请人： Yasuo Suda ,  Tomoaki Nishimura ,  Yuko Kishimoto ,  Sakiko Yamashita ,  Sachiko Tsuruta ,  Masahiro Wakao ,  Toshiomi Okuno

发明人： Yasuo Suda ,  Tomoaki Nishimura ,  Yuko Kishimoto ,  Sakiko Yamashita ,  Sachiko Tsuruta ,  Masahiro Wakao ,  Toshiomi Okuno

IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/569 ,  G01N33/543 ,  G01N33/92

CPC分类号： G01N33/569 ,  G01N33/5308 ,  G01N33/5432 ,  G01N33/68 ,  G01N33/92 ,  G01N2400/00

摘要： This invention provides a method of screening or patterning a biomaterial in terms of their specificities to sugar chains by performing real-time and comprehensive measurement of an interaction between sugar chains and the biomaterial concurrently with a very small amount of the biomaterial without labeling. It is a method of measuring an interaction between a biomaterial and a sugar chain(s), the method including: bringing a solution containing the biomaterial in contact with a ligand carrier, the ligand carrier including a support whose surface includes a metal, and a ligand conjugate(s) immobilized independently on the surface, the ligand carrier carrying the ligand conjugate(s) in such a manner that 1 to 500 kinds of the ligand conjugate(s) is immobilized per cm2, and each ligand conjugate having a structure in which a sugar chain is bonded with a linker compound having a sulfur atom, and the biomaterial being at least one selected from the groups of proteins, viruses, cells, microorganisms, liposome, and micelles.

摘要翻译： 本发明提供了一种通过与非标量的生物材料同时进行糖链与生物材料之间的相互作用的实时和全面测量来对其对糖链的特异性筛选或图案化生物材料的方法。 它是测量生物材料和糖链之间的相互作用的方法，该方法包括：使含有生物材料的溶液与配体载体接触，所述配体载体包括其表面包括金属的载体，和 配体缀合物独立地固定在表面上，配体载体携带配体缀合物，使得每cm 2固定1至500种配体缀合物，并且每个配体缀合物具有结构为 其中糖链与具有硫原子的连接体化合物结合，所述生物材料是选自蛋白质，病毒，细胞，微生物，脂质体和胶束中的至少一种。

公开(公告)号：US20110027854A1

公开(公告)日：2011-02-03

申请号：US12844742

申请日：2010-07-27

申请人： Yasuo SUDA ,  Masahiro Wakao ,  Takashi Kodama

发明人： Yasuo SUDA ,  Masahiro Wakao ,  Takashi Kodama

IPC分类号： C12N13/00 ,  H01F1/00 ,  C12M1/42

CPC分类号： C12N13/00 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B82Y25/00 ,  C12N1/20 ,  C12N7/00 ,  C12N2760/16151 ,  C12Q1/24 ,  G01N33/54326 ,  H01F1/0054

摘要： A method for concentrating viruses includes applying a magnetic force to a mixture containing: sugar chain-immobilized magnetic metal nano-particles each having a structure in which a sugar chain-immobilized metal nano-particle is bound to a first magnetic nano-particle; second magnetic particles with mean particle size larger than that of the sugar chain-immobilized magnetic metal nano-particles; and a specimen. Each sugar chain-immobilized metal nano-particle has a structure where a ligand-conjugate is bound to a metal nano-particle via sulfur atoms. The ligand-conjugate has a structure where a linker compound's amino group is connected to a sugar chain having a reducing terminal. The linker compound includes, in molecules thereof, an amino group, sulfur atoms, and a hydrocarbon chain having carbon-nitrogen bonds. This allows short-time concentration of viruses in a sufficient amount almost equal to that of centrifugation concentration, allowing safely and effectively concentrating target viruses, resulting in prompt, easy, and highly sensitive detection and identification of viruses.

摘要翻译： 用于浓缩病毒的方法包括：将磁力施加到含有固定有糖链的磁性金属纳米粒子的混合物，所述糖链固定的磁性金属纳米粒子具有与第一磁性纳米粒子结合的糖链固定金属纳米粒子的结构; 平均粒径大于固定链固定的金属纳米粒子的第二磁性颗粒; 和一个标本。 每个糖链固定的金属纳米颗粒具有其中配体 - 共轭物通过硫原子与金属纳米颗粒结合的结构。 配体 - 缀合物具有其中连接物化合物的氨基连接到具有还原末端的糖链的结构。 连接体化合物在其分子中包含氨基，硫原子和具有碳 - 氮键的烃链。 这允许短时间浓度的病毒足够的量几乎等于离心浓度，允许安全有效地浓缩目标病毒，导致迅速，容易和高度敏感的病毒检测和鉴定。

公开(公告)号：US20070213523A1

公开(公告)日：2007-09-13

申请号：US10588612

申请日：2005-02-04

申请人： Yasuo Suda ,  Akio Arano ,  Shoichi Kusumoto ,  Michael Sobel ,  Masahiro Wakao

发明人： Yasuo Suda ,  Akio Arano ,  Shoichi Kusumoto ,  Michael Sobel ,  Masahiro Wakao

IPC分类号： C07D339/04 ,  C07H3/04 ,  C07H3/06

CPC分类号： C07H15/04 ,  C07D339/04 ,  G01N33/54353

摘要： The present invention provides a novel linker compound which minimizes any nonspecific hydrophobic interactions and is capable of easily adjusting the length to a disulfide group subjected to metal bond to thereby enable effective formation of a metal-sulfur bond; novel ligand conjugate and ligand carrier, and a process for producing them. The linker compound is of a structure represented by the following general formula (1) where a, b, d, e are independently an integer of 0 to 6. X has a structure serving as a multi-branched structure moiety including three or more hydrocarbon derivative chains, wherein the hydrocarbon derivative chains each include an aromatic amino group at an end thereof, and may or may not include a carbon-nitrogen bond in a main chain thereof. The ligand conjugate includes the linker compound having a sugar molecule introduced therein.

摘要翻译： 本发明提供了一种新的接头化合物，其使任何非特异性疏水相互作用最小化，并且能够容易地将长度调节至经受金属键的二硫键，从而有效形成金属 - 硫键; 新型配体结合物和配体载体，及其制备方法。 连接体化合物为下述通式（1）所示的结构，其中a，b，d，e独立地为0〜6的整数。X具有作为多支链结构部分的结构，包含三个以上的烃 衍生链，其中所述烃衍生物链各自在其末端包括芳族氨基，并且在其主链中可以包括或不包括碳 - 氮键。 配体缀合物包括其中引入糖分子的连接体化合物。

公开(公告)号：US09464281B2

公开(公告)日：2016-10-11

申请号：US12844742

申请日：2010-07-27

申请人： Yasuo Suda ,  Masahiro Wakao ,  Takashi Kodama

发明人： Yasuo Suda ,  Masahiro Wakao ,  Takashi Kodama

IPC分类号： C12N7/02 ,  G01N33/566 ,  C12N13/00 ,  B03C1/01 ,  B03C1/28 ,  B82Y25/00 ,  C12N1/20 ,  C12N7/00 ,  C12Q1/24 ,  G01N33/543 ,  H01F1/00

CPC分类号： C12N13/00 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B82Y25/00 ,  C12N1/20 ,  C12N7/00 ,  C12N2760/16151 ,  C12Q1/24 ,  G01N33/54326 ,  H01F1/0054

摘要： A method for concentrating viruses includes applying a magnetic force to a mixture containing: sugar chain-immobilized magnetic metal nano-particles each having a structure in which a sugar chain-immobilized metal nano-particle is bound to a first magnetic nano-particle; second magnetic particles with mean particle size larger than that of the sugar chain-immobilized magnetic metal nano-particles; and a specimen. Each sugar chain-immobilized metal nano-particle has a structure where a ligand-conjugate is bound to a metal nano-particle via sulfur atoms. The ligand-conjugate has a structure where a linker compound's amino group is connected to a sugar chain having a reducing terminal. The linker compound includes, in molecules thereof, an amino group, sulfur atoms, and a hydrocarbon chain having carbon-nitrogen bonds. This allows short-time concentration of viruses in a sufficient amount almost equal to that of centrifugation concentration, allowing safely and effectively concentrating target viruses, resulting in prompt, easy, and highly sensitive detection and identification of viruses.

摘要翻译： 用于浓缩病毒的方法包括：将磁力施加到含有固定有糖链的磁性金属纳米粒子的混合物，所述糖链固定的磁性金属纳米粒子具有与第一磁性纳米粒子结合的糖链固定金属纳米粒子的结构; 平均粒径大于固定链固定的金属纳米粒子的第二磁性颗粒; 和一个标本。 每个糖链固定的金属纳米颗粒具有其中配体 - 共轭物通过硫原子与金属纳米颗粒结合的结构。 配体 - 缀合物具有其中连接物化合物的氨基连接到具有还原末端的糖链的结构。 连接体化合物在其分子中包含氨基，硫原子和具有碳 - 氮键的烃链。 这允许短时间浓度的病毒足够的量几乎等于离心浓度，允许安全有效地浓缩目标病毒，导致迅速，容易和高度敏感的病毒检测和鉴定。

公开(公告)号：US07838549B2

公开(公告)日：2010-11-23

申请号：US10588612

申请日：2005-02-04

申请人： Yasuo Suda ,  Akio Arano ,  Shoichi Kusumoto ,  Michael Sobel ,  Masahiro Wakao

发明人： Yasuo Suda ,  Akio Arano ,  Shoichi Kusumoto ,  Michael Sobel ,  Masahiro Wakao

IPC分类号： A61K31/385 ,  C07D409/14

CPC分类号： C07H15/04 ,  C07D339/04 ,  G01N33/54353

摘要： The present invention provides a novel linker compound which minimizes any nonspecific hydrophobic interactions and is capable of easily adjusting the length to a disulfide group subjected to metal bond to thereby enable effective formation of a metal-sulfur bond; novel ligand conjugate and ligand carrier, and a process for producing them. The linker compound is of a structure represented by the following general formula (1) where a, b, d, e are independently an integer of 0 to 6. X has a structure serving as a multi-branched structure moiety including three or more hydrocarbon derivative chains, wherein the hydrocarbon derivative chains each include an aromatic amino group at an end thereof, and may or may not include a carbon-nitrogen bond in a main chain thereof. The ligand conjugate includes the linker compound having a sugar molecule introduced therein.

摘要翻译： 本发明提供了一种新的接头化合物，其使任何非特异性疏水相互作用最小化，并且能够容易地将长度调节至经受金属键的二硫键，从而有效形成金属 - 硫键; 新型配体结合物和配体载体，及其制备方法。 连接体化合物为下述通式（1）所示的结构，其中a，b，d，e独立地为0〜6的整数。X具有作为多支链结构部分的结构，包含三个以上的烃 衍生链，其中所述烃衍生物链各自在其末端包括芳族氨基，并且在其主链中可以包括或不包括碳 - 氮键。 配体缀合物包括其中引入糖分子的连接体化合物。