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    Efficient process for producing dumbbell DNA
    1.
    发明授权
    Efficient process for producing dumbbell DNA 有权
    生产哑铃DNA的有效方法

    公开(公告)号：US07972816B2

    公开(公告)日：2011-07-05

    申请号：US10567168

    申请日：2004-08-09

    申请人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

    发明人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N15/66 ,  A61K31/711 ,  A61K31/713 ,  C12N15/10 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/53 ,  C12N2330/30

    摘要： The present invention provides a simple method for producing a dumbbell-shaped DNA.A method for producing a dumbbell-shaped DNA, wherein each of sense and antisense strands is connected at both the 5′ and 3′ ends of a linear-shaped double stranded DNA by a single stranded DNA of loop structure, comprising the steps of; 1) amplifying a target DNA in a template DNA by PCR using sense and antisense primers, wherein each of the sense and antisense primers contains the following sequence (a) at the 5′ end and also contains the following sequences (b), (c), and (d) in order from the 5′ end to the 3′ end, (a) a part of a sense sequence of a nickase recognition sequence, comprising the sequence of a region between the site where a nick is introduced by the action of a nickase and the 3′ end, (b) a sequence capable of forming a loop structure from a single strand, (c) the entire antisense sequence of the nickase recognition sequence (a), (d) a sequence complementary to all or part of the sequence of the target DNA; 2) treating the amplified DNA product of step 1) with a nickase of (a); 3) heating and then annealing the nickase treated amplified DNA product of step 2); and 4) treating the heated and annealed amplified DNA product of step 3) with DNA ligase, wherein the sense and antisense primers used in step 1) are phosphorylated at the 5′ end, or the amplified DNA product is phosphorylated at the 5′ end after step 1) but before step 4).

    摘要翻译： 本发明提供了一种制备哑铃型DNA的简单方法。 一种制备哑铃型DNA的方法，其中有义链和反义链中的每一个通过环结构的单链DNA连接在线形双链DNA的5'和3'末端，包括以下步骤： 1）使用正义和反义引物通过PCR扩增模板DNA中的靶DNA，其中有义和反义引物中的每一个在5'末端包含以下序列（a），并且还包含以下序列（b），（c ）和（d）从5'末端到3'末端的顺序，（a）切口酶识别序列的有义序列的一部分，其包含在切口被引入的位点之间的区域的序列 切口酶和3'端的作用，（b）能够从单链形成环结构的序列，（c）切口酶识别序列（a）的整个反义序列，（d）与所有的互补序列互补的序列 或靶DNA的序列的一部分; 2）用（a）的切口酶处理步骤1）的扩增的DNA产物; 3）加热然后对步骤2）的经切割处理的扩增的DNA产物进行退火; 和4）用DNA连接酶处理步骤3）的加热和退火的扩增DNA产物，其中步骤1）中使用的有义和反义引物在5'末端被磷酸化，或者扩增的DNA产物在5'末端被磷酸化 在步骤1之后），但在步骤4之前）。

    Efficient Process For Producing Dumbbell Dna
    2.
    发明申请
    Efficient Process For Producing Dumbbell Dna 有权
    生产哑铃Dna的高效过程

    公开(公告)号：US20080153763A1

    公开(公告)日：2008-06-26

    申请号：US10567168

    申请日：2004-08-09

    申请人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

    发明人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

    IPC分类号： A61K31/711 ,  C12P19/34 ,  C12N15/64 ,  C07H21/04

    CPC分类号： C12N15/66 ,  A61K31/711 ,  A61K31/713 ,  C12N15/10 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/53 ,  C12N2330/30

    摘要： The present invention provides a simple method for producing a dumbbell-shaped DNA.A method for producing a dumbbell-shaped DNA, wherein each of sense and antisense strands is connected at both the 5′ and 3′ ends of a linear-shaped double stranded DNA by a single stranded DNA of loop structure, comprising the steps of; 1) amplifying a target DNA in a template DNA by PCR using sense and antisense primers, wherein each of the sense and antisense primers contains the following sequence (a) at the 5′ end and also contains the following sequences (b), (c), and (d) in order from the 5′ end to the 3′ end, (a) a part of a sense sequence of a nickase recognition sequence, comprising the sequence of a region between the site where a nick is introduced by the action of a nickase and the 3′ end, (b) a sequence capable of forming a loop structure from a single strand, (c) the entire antisense sequence of the nickase recognition sequence (a), (d) a sequence complementary to all or part of the sequence of the target DNA; 2) treating the amplified DNA product of step 1) with a nickase of (a); 3) heating and then annealing the nickase treated amplified DNA product of step 2); and 4) treating the heated and annealed amplified DNA product of step 3) with DNA ligase, wherein the sense and antisense primers used in step 1) are phosphorylated at the 5′ end, or the amplified DNA product is phosphorylated at the 5′ end after step 1) but before step 4).

    摘要翻译： 本发明提供了一种制备哑铃型DNA的简单方法。 一种制备哑铃型DNA的方法，其中有义链和反义链中的每一个通过环结构的单链DNA连接在线形双链DNA的5'和3'末端，包括以下步骤： 1）使用正义和反义引物通过PCR扩增模板DNA中的靶DNA，其中有义和反义引物中的每一个在5'末端包含以下序列（a），并且还包含以下序列（b），（c ）和（d）从5'末端到3'末端的顺序，（a）切口酶识别序列的有义序列的一部分，其包含在切口被引入的位点之间的区域的序列 切口酶和3'端的作用，（b）能够从单链形成环结构的序列，（c）切口酶识别序列（a）的整个反义序列，（d）与所有的互补序列互补的序列 或靶DNA的序列的一部分; 2）用（a）的切口酶处理步骤1）的扩增的DNA产物; 3）加热然后对步骤2）的经切割处理的扩增的DNA产物进行退火; 和4）用DNA连接酶处理步骤3）的加热和退火的扩增DNA产物，其中步骤1）中使用的有义和反义引物在5'末端被磷酸化，或者扩增的DNA产物在5'末端被磷酸化 在步骤1之后），但在步骤4之前）。

    NON-AQUEOUS ELECTROLYTE SECONDARY CELL
    3.
    发明申请
    NON-AQUEOUS ELECTROLYTE SECONDARY CELL 审中-公开
    非水溶性电解质二次电池

    公开(公告)号：US20110159344A1

    公开(公告)日：2011-06-30

    申请号：US12977934

    申请日：2010-12-23

    申请人： Kazuma Kobayashi ,  Yohei Hirota ,  Yuki Morikawa ,  Yoshio Kato ,  Yasuyuki Kusumoto ,  Shigeki Matsuta

    发明人： Kazuma Kobayashi ,  Yohei Hirota ,  Yuki Morikawa ,  Yoshio Kato ,  Yasuyuki Kusumoto ,  Shigeki Matsuta

    IPC分类号： H01M2/16 ,  H01M2/18

    CPC分类号： C09J7/38 ,  C09J7/22 ,  C09J2201/622 ,  C09J2409/00 ,  C09J2479/086 ,  H01M4/13 ,  H01M4/366 ,  H01M4/505 ,  H01M4/525 ,  H01M4/62 ,  H01M10/052

    摘要： According to the invention, there can be provided a non-aqueous electrolyte secondary cell whose capacity is hardly decreased even stored at high temperatures in a charged state.The non-aqueous electrolyte secondary cell uses an insulation adhesive tape composed of a base material and a glue material. And in an absorbance spectra of the glue material measured using an infrared spectrophotometer so that the maximum peak intensity is 5 to 20% in transmittance, when peak intensities for C—H stretching vibration of 3040 to 2835 cm−1 and C═O stretching vibration of 1870 to 1560 cm−1 are respectively defined as I(C—H) and I(C═O), a peak intensity ratio represented by I(C═O)/I(C—H) is 0.01 or less.

    摘要翻译： 根据本发明，可以提供即使在充电状态下在高温下储存的能力几乎不降低的非水电解质二次电池。 非水电解质二次电池使用由基材和胶合材料构成的绝缘胶带。 在使用红外分光光度计测定的胶料的吸收光谱中，透射率的最大峰值强度为5〜20％时，C-H伸缩振动的峰值强度为3040〜2835cm -1，C = O伸缩振动 分别定义为I（C-H）和I（C = O），I（C = O）/ I（C-H）表示的峰强度比为0.01以下。

    Stator
    4.
    发明申请
    Stator 有权
    定子

    公开(公告)号：US20080258570A1

    公开(公告)日：2008-10-23

    申请号：US12150059

    申请日：2008-04-23

    申请人： Kazuyuki Yamaguchi ,  Kenji Endo ,  Yoshio Kato

    发明人： Kazuyuki Yamaguchi ,  Kenji Endo ,  Yoshio Kato

    IPC分类号： H02K3/48

    CPC分类号： H02K15/067

    摘要： The present invention provides a stator coiled in a spiral, capable of mechanically inserting a wound coil in the slot of a stator core. In a stator in which coils are overlapped in a spiral when viewed from the end surface of the stator core after each of one sides of a plurality of coils wound in advance is inserted in the slot of the stator core and each of the other sides of the plurality of coils is inserted in a different slot, by repeating the inserting operation twice or more, the loop of the coils overlapped in a spiral are piled in two layers or more in the radius direction of the stator core and the total coil sectional area inserted in the slots of the stator core is larger than a prescribed value based on the inside radius of the stator core.

    摘要翻译： 本发明提供一种螺旋状的定子，其能够将缠绕的线圈机械地插入定子芯的槽中。 在从预定的多个线圈的一侧的每一侧插入定子铁芯的槽中的每一个侧面之后，在从定子铁心的端面观察时，线圈重叠成螺旋状的定子中， 通过重复插入操作两次以上，将多个线圈插入到不同的槽中，以螺旋重叠的线圈的环在定子铁芯的半径方向上堆叠成两层以上，总线圈截面积 基于定子铁心的内半径插入定子铁芯的槽中的规定值大于规定值。

    Magnetostrictive device for torque sensor and method of manufacture of the same
    7.
    发明授权
    Magnetostrictive device for torque sensor and method of manufacture of the same 失效
    用于扭矩传感器的磁致伸缩装置及其制造方法

    公开(公告)号：US06595073B1

    公开(公告)日：2003-07-22

    申请号：US09512804

    申请日：2000-02-25

    申请人： Wataru Yagi ,  Kouta Maruyama ,  Atsunao Itoh ,  Yoshio Kato

    发明人： Wataru Yagi ,  Kouta Maruyama ,  Atsunao Itoh ,  Yoshio Kato

    IPC分类号： G01L314

    CPC分类号： G01L3/102 ,  G01L3/103 ,  G01L3/105 ,  Y10T29/49004

    摘要： A magnetostrictive device for a torque sensor which has a high sensor sensitivity whether the excitation frequency is in a high frequency range or in a low frequency range and can withstand excess load torques well, and also a method of easily manufacturing the device. In manufacturing the magnetostrictive device 1 for a torque sensor, which has a magnetic anisotropic portion 15 formed on the surface of a shaft body 10 thereof, the method of forming the magnetic anisotropic portion 15 comprises the steps of: forming spiral grooves in the surface of the shaft body 10 to form a plurality of troughs 16 and ridges 17 alternately; and directly applying a compressive stress only to the ridges 17 to cause deformations accompanied by plastic flow and to provide an almost planar plateau portion 171 at a crest portion of each of the ridges 17.

    摘要翻译： 一种用于扭矩传感器的磁致伸缩装置，无论激发频率是在高频范围还是在低频范围内，传感器灵敏度高，能够很好地承受过多的负载转矩，还有一种易于制造该装置的方法。 在制造具有形成在其轴体10的表面上的磁各向异性部分15的扭矩传感器的磁致伸缩装置1中，形成磁各向异性部分15的方法包括以下步骤：在 轴体10交替地形成多个槽16和脊17; 并且仅将压缩应力仅直接施加到脊部17以引起伴随塑性流动的变形，并且在每个脊部17的波峰部分处提供几乎平坦的平台部分171。