摘要:
To provide a plasmid vector having high productivity which can be used for large scale production of an industrially valuable and useful protein, and a transformant using the plasmid vector. A plasmid vector having DNA which encodes a plasmid replication protein in which one or more amino acid residues that are selected from (a) position 48, (b) position 262, (c) position 149 and (d) position 198 in the amino acid sequence represented by SEQ ID NO: 2 are substituted with (a) Ala, Gly, Thr, Arg, Glu, Asn or Gln, (b) Gly, Ser, Thr, Cys or Val, (c) Asn, and (d) Glu, respectively.
摘要翻译:提供可用于大规模生产工业上有价值且有用的蛋白质的高生产率的质粒载体,以及使用质粒载体的转化体。 一种质粒载体,其具有编码质粒复制蛋白的质粒载体,其中一个或多个选自(a)位置48,(b)位置262,(c)位置149和(d)位置198的氨基酸残基 由(a)Ala,Gly,Thr,Arg,Glu,Asn或Gln,(b)Gly,Ser,Thr,Cys或Val,(c)Asn和(d)代替SEQ ID NO: Glu。
摘要:
To provide a plasmid vector having high productivity which can be used for large scale production of an industrially valuable and useful protein, and a transformant using the plasmid vector. A plasmid vector having DNA which encodes a plasmid replication protein in which one or more amino acid residues that are selected from (a) position 48, (b) position 262, (c) position 149 and (d) position 198 in the amino acid sequence represented by SEQ ID NO: 2 are substituted with (a) Ala, Gly, Thr, Arg, Glu, Asn or Gln, (b) Gly, Ser, Thr, Cys or Val, (c) Asn, and (d) Glu, respectively.
摘要翻译:提供可用于大规模生产工业上有价值且有用的蛋白质的高生产率的质粒载体,以及使用质粒载体的转化体。 一种质粒载体,其具有编码质粒复制蛋白的质粒载体,其中一个或多个选自(a)位置48,(b)位置262,(c)位置149和(d)位置198的氨基酸残基 由(a)Ala,Gly,Thr,Arg,Glu,Asn或Gln,(b)Gly,Ser,Thr,Cys或Val,(c)Asn和(d)代替SEQ ID NO: Glu。
摘要:
Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译:在本发明中提供的是碱性蛋白酶,其中(a)位置65,(b)位置101,(c)位置163,(d)位置170,(e)位置171,(f) ,(g)位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(c):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, 位置(d):缬氨酸和亮氨酸,位置(e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(f):异亮氨酸,甘氨酸和苏氨酸,位置(g) 位置(h):丝氨酸,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺,位置(i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):缬氨酸,缬氨酸,苏氨酸,亮氨酸,异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶,即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质,皮脂等的复合污渍,因此可用作 要掺入洗涤剂中的酶。
摘要:
Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译:在本发明中提供的是碱性蛋白酶,其中(a)位置65,(b)位置101,(c)位置163,(d)位置170,(e)位置171,(f) ,(g)位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(c):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, 位置(d):缬氨酸和亮氨酸,位置(e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(f):异亮氨酸,甘氨酸和苏氨酸,位置(g) 位置(h):丝氨酸,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺,位置(i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):缬氨酸,缬氨酸,苏氨酸,亮氨酸,异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶,即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质,皮脂等的复合污渍,因此可用作 要掺入洗涤剂中的酶。
摘要:
An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. A method to an alkaline protease having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore useful as an ingredient in a detergent.
摘要翻译:(a)位置65,(b)位置101,(c)位置163,(d)位置17-0,(e)位置171,(f)位置273,(g) 位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(e):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, (e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(K):异亮氨酸,甘氨酸和苏氨酸,位置(g):缬氨酸和亮氨酸, (i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):ine碱,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺。 一种碱性蛋白酶的方法,即使在高度浓缩的脂肪酸的存在下也具有活性,并且对于去除含有蛋白质,皮脂等的复合污渍具有优异的去污力,因此可用作洗涤剂中的成分。
摘要:
An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 63, (b) position 89, (c) position 120, (d) positions 63 and 187, (e) position 226, (f) position 296, (g) position 304 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): serine, (b): histidine, (c): arginine, (d): serine,(e): tyrosine, (f): valine, and (g): serine; and a gene encoding the alkaline protease.
摘要翻译:一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶,其选自(a)位置63,(b)位置89,(c)位置120,(d)位置63和187,(e)位置 226,(f)位置296,(g)SEQ ID NO:1的氨基酸序列的位置304或与这些位置相应的位置分别是以下氨基酸残基:(a):丝氨酸,(b ):组氨酸,(c):精氨酸,(d):丝氨酸,(e):酪氨酸,(f):缬氨酸和(g):丝氨酸; 和编码碱性蛋白酶的基因。
摘要:
An alkaline protease having an amino acid, wherein one or more amino acid residues selected from among those located at (a) position 63, (b) position 89, (c) position 120, (d) positions 63 and 187, (e) position 226, (f) position 296, (g) position 304 of the amino acid sequence of SEQ NO; 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): serine, (b): histidine, (c): arginine, (d): serine, (e): tyrosine, (f): valine, and (g): serine; and a gene encoding the alkaline protease. The alkaline protease has for example, excellent detergency against complex soil and has improved thermal stability.
摘要翻译:具有氨基酸的碱性蛋白酶,其中选自位于(a)位置63,(b)位置89,(c)位置120,(d)位置63和187的那些中的一个或多个氨基酸残基,(e) 位置226,(f)位置296,(g)SEQ ID NO:1的氨基酸序列的位置304; (a):丝氨酸,(b):组氨酸,(c):精氨酸,(d):丝氨酸,(e):酪氨酸,(f ):缬氨酸和(g):丝氨酸; 和编码碱性蛋白酶的基因。 碱性蛋白酶具有例如对复杂土壤的优异的去污力并且具有改善的热稳定性。
摘要:
The present invention provides an enzyme-containing granulated product containing, in a uniformly dispersed state, an enzyme and one or more stabilizers selected from the group consisting of reducing agents and antioxidants. The invention also discloses a method for the production of the granulated product, as well as bleaching agents and detergent compositions containing the granulated product. The granulated product loses only a minimal level of enzyme activity even in the copresence of a bleaching agent, and exhibits excellent solubility. Therefore, bleach compositions and detergent compositions prepared by incorporating the granulated product fully exhibit the enzyme activity and bleaching activity.