公开(公告)号：US10920192B2

公开(公告)日：2021-02-16

申请号：US14259997

申请日：2014-04-23

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub Hanna ,  Noa Novershtern ,  Yoach Rais

IPC: C12N5/0735 ,  C12N5/074

Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

公开(公告)号：US11920164B2

公开(公告)日：2024-03-05

申请号：US15500163

申请日：2015-07-30

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub Hanna ,  Noa Novershtern ,  Yoach Rais

IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.

公开(公告)号：US20140315301A1

公开(公告)日：2014-10-23

申请号：US14259997

申请日：2014-04-23

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa Novershtern ,  Yoach Rais

IPC: C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0611 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/42 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/999

Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

Abstract translation: 提供了一种分离的人类天真多能干细胞（PSC），其中：（i）当初始PSC是女性PSC时，所述初始女性PSC具有X-非活性特异性转录物（XIST）基因的两个未甲基化等位基因; 和（ii）当所述初始PSC是雄性PSC时，所述初始雄性PSC具有所述XIST基因的未甲基化等位基因。 还提供了包含ERK1 / 2抑制剂，GSK3β抑制剂，p38抑制剂，JNK抑制剂，STAT3活化剂和至少一种选自以下的药剂的培养基：bFGF，TGFβ1，PKC抑制剂， ROCK抑制剂和NOTCH抑制剂; 或至少选自TGFR抑制剂，FGFR抑制剂，PKC抑制剂，ROCK抑制剂和NOTCH抑制剂的药剂。