公开(公告)号：US10920192B2

公开(公告)日：2021-02-16

申请号：US14259997

申请日：2014-04-23

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub Hanna ,  Noa Novershtern ,  Yoach Rais

IPC: C12N5/0735 ,  C12N5/074

Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

公开(公告)号：US11920164B2

公开(公告)日：2024-03-05

申请号：US15500163

申请日：2015-07-30

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub Hanna ,  Noa Novershtern ,  Yoach Rais

IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.

公开(公告)号：US11959097B2

公开(公告)日：2024-04-16

申请号：US18226877

申请日：2023-07-27

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub Hanna ,  Sergey Viukov ,  Emilie Wildschutz ,  Noa Novershtern ,  Carine Joubran ,  Segev Naveh Tassa ,  Alejandro Castrejon Aguilera ,  Bernardo Oldak ,  Shadi Tarazi ,  Francesco Roncato

IPC: C12N5/073

CPC classification number: C12N5/0604 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2506/03

Abstract: Methods of generating a synthetic embryo are provided. Accordingly, there is provided a method of generating a synthetic embryo comprising inducing expression of a factor that induces differentiation to trophectoderm cells in a subpopulation of naïve pluripotent stem cells (PSCs) to obtain a trophectoderm primed cells; inducing expression of a factor that induces differentiation to extra embryonic primitive endodermal cells in a second subpopulation of naïve PSCs to obtain extra embryonic primitive endodermal primed cells; and mixing said trophectoderm primed cells and said extra embryonic primitive endodermal primed cells with naïve PSCs under conditions that allow formation of aggregated cells. Also provided are articles of manufactures, mixtures and aggregates of cells and methods of using same.

公开(公告)号：US20140315301A1

公开(公告)日：2014-10-23

申请号：US14259997

申请日：2014-04-23

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa Novershtern ,  Yoach Rais

IPC: C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0611 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/42 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/999

Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

Abstract translation: 提供了一种分离的人类天真多能干细胞（PSC），其中：（i）当初始PSC是女性PSC时，所述初始女性PSC具有X-非活性特异性转录物（XIST）基因的两个未甲基化等位基因; 和（ii）当所述初始PSC是雄性PSC时，所述初始雄性PSC具有所述XIST基因的未甲基化等位基因。 还提供了包含ERK1 / 2抑制剂，GSK3β抑制剂，p38抑制剂，JNK抑制剂，STAT3活化剂和至少一种选自以下的药剂的培养基：bFGF，TGFβ1，PKC抑制剂， ROCK抑制剂和NOTCH抑制剂; 或至少选自TGFR抑制剂，FGFR抑制剂，PKC抑制剂，ROCK抑制剂和NOTCH抑制剂的药剂。