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公开(公告)号：US20240010973A1
公开(公告)日：2024-01-11
申请号：US18226877
申请日：2023-07-27
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Sergey VIUKOV , Emilie WILDSCHUTZ , Noa NOVERSHTERN , Carine JOUBRAN , Segev NAVEH TASSA , Alejandro CASTREJON AGUILERA , Bernardo OLDAK , Shadi TARAZI , Francesco RONCATO
IPC: C12N5/073
CPC classification number: C12N5/0604 , C12N2506/03 , C12N2501/155 , C12N2501/115 , C12N2501/119 , C12N2501/91 , C12N2500/02
Abstract: Methods of generating a synthetic embryo are provided. Accordingly, there is provided a method of generating a synthetic embryo comprising inducing expression of a factor that induces differentiation to trophectoderm cells in a subpopulation of naïve pluripotent stem cells (PSCs) to obtain a trophectoderm primed cells; inducing expression of a factor that induces differentiation to extra embryonic primitive endodermal cells in a second subpopulation of naïve PSCs to obtain extra embryonic primitive endodermal primed cells; and mixing said trophectoderm primed cells and said extra embryonic primitive endodermal primed cells with naïve PSCs under conditions that allow formation of aggregated cells. Also provided are articles of manufactures, mixtures and aggregates of cells and methods of using same.
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公开(公告)号：US20250101375A1
公开(公告)日：2025-03-27
申请号：US18825336
申请日：2024-09-05
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Sergey VIUKOV , Emilie WILDSCHUTZ , Noa NOVERSHTERN , Carine JOUBRAN , Segev NAVEH TASSA , Alejandro CASTREJON AGUILERA , Bernardo OLDAK , Shadi TARAZI , Francesco RONCATO
Abstract: Methods of generating a synthetic embryo are provided. Accordingly, there is provided a method of generating a synthetic embryo comprising inducing expression of a factor that induces differentiation to trophectoderm cells in a subpopulation of naïve pluripotent stem cells (PSCs) to obtain a trophectoderm primed cells; inducing expression of a factor that induces differentiation to extra embryonic primitive endodermal cells in a second subpopulation of naïve PSCs to obtain extra embryonic primitive endodermal primed cells; and mixing said trophectoderm primed cells and said extra embryonic primitive endodermal primed cells with naïve PSCs under conditions that allow formation of aggregated cells. Also provided are articles of manufactures, mixtures and aggregates of cells and methods of using same.
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公开(公告)号：US20210363495A1
公开(公告)日：2021-11-25
申请号：US17382500
申请日：2021-07-22
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Noa NOVERSHTERN , Tom Haim SHANI , Sergey VIUKOV , Rada MASSARWA , Jonathan BAYERL , Muneef AYYASH
IPC: C12N5/074
Abstract: A culture medium comprising a WNT inhibitor, a SRC inhibitor and a protein kinase C (PKC) inhibitor is disclosed. The medium is devoid of an amount of GSK3beta inhibitor that increases beta-catenin translocation to the nucleus of a pluripotent stem cell being cultured in the culture medium. Uses thereof are also disclosed.
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Patent Agency Ranking
公开(公告)号：US20240026262A1
公开(公告)日：2024-01-25
申请号：US18369233
申请日：2023-09-18
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA
Abstract: Methods and devices for ex-utero mouse embryonic development are provided. Accordingly, there is provided a method of ex-utero culturing a mouse embryo at a zygote stage under conditions that allow developments of the embryo to organogenesis or any developmental stage therein-between. Also provided a fetal incubation system, and methods of using same.
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公开(公告)号：US20140315301A1
公开(公告)日：2014-10-23
申请号：US14259997
申请日：2014-04-23
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Noa Novershtern , Yoach Rais
IPC: C12N5/0735 , C12N5/074
CPC classification number: C12N5/0611 , C12N5/0606 , C12N5/0696 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/2306 , C12N2501/235 , C12N2501/42 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/727 , C12N2501/999
Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.
Abstract translation: 提供了一种分离的人类天真多能干细胞（PSC），其中：（i）当初始PSC是女性PSC时，所述初始女性PSC具有X-非活性特异性转录物（XIST）基因的两个未甲基化等位基因; 和（ii）当所述初始PSC是雄性PSC时，所述初始雄性PSC具有所述XIST基因的未甲基化等位基因。 还提供了包含ERK1 / 2抑制剂，GSK3β抑制剂，p38抑制剂，JNK抑制剂，STAT3活化剂和至少一种选自以下的药剂的培养基：bFGF，TGFβ1，PKC抑制剂， ROCK抑制剂和NOTCH抑制剂; 或至少选自TGFR抑制剂，FGFR抑制剂，PKC抑制剂，ROCK抑制剂和NOTCH抑制剂的药剂。
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公开(公告)号：US20240318144A1
公开(公告)日：2024-09-26
申请号：US18433553
申请日：2024-02-06
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Noa NOVERSHTERN , Yoach RAIS
IPC: C12N5/074 , C12N5/00 , C12N5/0735
CPC classification number: C12N5/0696 , C12N5/0037 , C12N5/0606 , C12N2500/38 , C12N2500/90 , C12N2501/01 , C12N2501/115 , C12N2501/125 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/235 , C12N2501/415 , C12N2501/50 , C12N2501/60 , C12N2501/71 , C12N2501/72 , C12N2501/727 , C12N2501/73 , C12N2501/999 , C12N2506/00
Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.
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公开(公告)号：US20210253998A1
公开(公告)日：2021-08-19
申请号：US17117157
申请日：2020-12-10
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Noa NOVERSHTERN , Yoach RAIS
IPC: C12N5/0735 , C12N5/074
Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.
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公开(公告)号：US20170275593A1
公开(公告)日：2017-09-28
申请号：US15500163
申请日：2015-07-30
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Yaqub HANNA , Noa NOVERSHTERN , Yoach RAIS
IPC: C12N5/074 , C12N5/00 , C12N5/0735
CPC classification number: C12N5/0696 , C12N5/0037 , C12N5/0606 , C12N2500/38 , C12N2500/90 , C12N2501/01 , C12N2501/115 , C12N2501/125 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/235 , C12N2501/415 , C12N2501/50 , C12N2501/60 , C12N2501/71 , C12N2501/72 , C12N2501/727 , C12N2501/73 , C12N2501/999 , C12N2506/00
Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.
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